Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,627,299	2 bp→GA	intergenic (+5/‑15)	radA → / → nadR	DNA recombination protein/DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,299	0	A	G	100.0%	84.7 / NA	26	intergenic (+5/‑16)	radA/nadR	DNA recombination protein/DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/14);  total (12/14)
*	NC_000913	4,627,300	0	T	A	100.0%	78.0 / NA	26	intergenic (+6/‑15)	radA/nadR	DNA recombination protein/DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/14);  total (12/14)

CCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCC  >  NC_000913/4627063‑4627538                                                                                                                                                                                                                                             ||                                                                                                                                                                                                                                               ccTGCGTGACAGACCTGTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGgaga                                                                                                                                                                                                                                   <  1:103437/251‑1 (MQ=255)   tGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGat                                                                                                                                                                                                                                  >  2:29019/1‑250 (MQ=255)              ccGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCCGGGGAGATCCGCCCGGTGCCCCGCGGTAAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCAGGCGGCTAACGTACCGACAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTAGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTAAttt                                                                                                                                                                                                                      >  2:120269/1‑251 (MQ=255)                       cAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTg                                                                                                                                                                                                             >  1:164934/1‑251 (MQ=255)                                  ggtgTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCa                                                                                                                                                                                                   <  1:157466/250‑1 (MQ=255)                                                  gggcgggcAGGGGAGATCCGCCCGGTGCCCAGCGGGCAGGAACTAATGTCAGAAGCGGCGAAACACGGTTTGCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATGTTTGGCGTTAAAAAACTCTCCGACGCTCTTAGCGTGTTCGACGACTTACAATGAGGAATACggaggg                                                                                                                                                                                                                                      <  2:232327/199‑1 (MQ=255)                                                  gggCTGGCAGGGGAGATCCGCCAGGTGCCCAGCGGTCAGGAACGCATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAGGGGATGCAGATTTTTGGCGTTAAACAACTCTCCGACGCGCTTAGCGTGTTAGACGACTTGTAATGAGGAATACggaggg                                                                                                                                                                                                                                      >  1:232327/1‑199 (MQ=255)                                                             ggAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTAcagca                                                                                                                                                                       <  2:57114/251‑1 (MQ=255)                                                                                aGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGt                                                                                                                                                    <  1:165583/251‑1 (MQ=255)                                                                                        ggcaCGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGCCGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCa                                                                                                                                             >  2:70696/4‑250 (MQ=255)                                                                                                          ggcgAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGtt                                                                                                                          <  1:235801/251‑1 (MQ=255)                                                                                                              aaaCACGGTTTTCGCCGGGCGATTGATCCGGCGGCTACCGTACCGAAAAAAGCGCCGGAAGGGATGCAGCTTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACATGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCGGTTACTg                                                                                                                      >  2:251701/1‑251 (MQ=255)                                                                                                                  acGGTTTTCGCCGGGCGATTGGTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGGTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACGTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATg                                                                                                                  <  2:131679/251‑1 (MQ=255)                                                                                                                     gTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATTACCAAAGGGTATTTAAGCCAGTTACTGAATGcc                                                                                                                >  2:80275/1‑250 (MQ=255)                                                                                                                          cGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGCCCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATc                                                                                                          <  1:5262/251‑1 (MQ=255)                                                                                                                                gCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGc                                                                                                    >  1:62854/1‑251 (MQ=255)                                                                                                                                     tGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGGAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGGCTTTAAGCCAGTTACTGAATGCCAAAATCAAAAGccca                                                                                                 >  2:6307/1‑248 (MQ=255)                                                                                                                                               gcTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAg                                                                                     <  2:216232/251‑1 (MQ=255)                                                                                                                                                  aaCGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTg                                                                                  <  1:42788/251‑1 (MQ=255)                                                                                                                                                  aaCGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTg                                                                                  <  2:79245/251‑1 (MQ=255)                                                                                                                                                     gtaccGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGCATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTgg                                                                                 >  2:142292/4‑249 (MQ=255)                                                                                                                                                              aaaGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGAATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCAc                                                                      >  1:168052/1‑251 (MQ=255)                                                                                                                                                                                                                   tAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCAGCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTa                   <  1:262005/249‑1 (MQ=255)                                                                                                                                                                                                                      cGTGTTCGTCGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTAGTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTGTGGGGGCTTGAGTTTCCCCTGCAGAAGAAAGCCATTGGTGTTGTATTCgg              <  2:174006/251‑1 (MQ=255)                                                                                                                                                                                                                                 acTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTAcc   <  1:80275/251‑1 (MQ=255)                                                                                                                                                                                                                                  cTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTAGGTAAGTTCTAcac  >  1:54259/1‑249 (MQ=255)                                                                                                                                                                                                                                             ||                                                                                                                                                                                                                                               CCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCC  >  NC_000913/4627063‑4627538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑