| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,441 | 2 bp→AG | coding (708‑709/1287 nt) | thrC → | threonine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,441 | 0 | G | A | 100.0% | 62.4 / NA | 21 | E236E (GAG→GAA) | thrC | threonine synthase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (8/13); total (8/13) | |||||||||||
| * | NC_000913 | 4,442 | 0 | A | G | 100.0% | 60.6 / NA | 21 | T237A (ACG→GCG) | thrC | threonine synthase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (8/13); total (8/13) | |||||||||||
GGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATT‑GCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGC > NC_000913/4211‑4684 || ggCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGt > 1:63603/1‑251 (MQ=255) gTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGACGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGcc > 1:227322/1‑250 (MQ=255) gCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCgg < 2:63603/249‑1 (MQ=255) cAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGATGGTTGTCTCGGTGCCAAGCGGaa > 1:133867/1‑250 (MQ=255) gTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTg < 2:252302/248‑1 (MQ=255) cAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTgg < 1:128419/251‑1 (MQ=255) cGAAACTGCTGCCATCGACGGCGATTTCGATGCCTGCCAGGCGCAGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACGCGATTAACATCAGCCGTTTGGTGGCGCAGATTTGCTACTACGTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGTAAACTTCGGCGATTTGACGGCGTGTCTGCTGGCGAAg < 2:37236/250‑1 (MQ=255) gTTGCCAACGACGGCGATTTCGAGGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACAGCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCgg < 1:37719/251‑1 (MQ=255) ggCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGCTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTg > 1:267315/1‑166 (MQ=255) ggCGCTGGTGAAGCAGGCGTTTGATGATGAAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACAGCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCTCAGGAAGCGTGGAACCAGCTGGTTGTCTCGGTg < 2:267315/166‑1 (MQ=255) atgaAGAGCTGAAAGTGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATT‑GCTGCGACCAACGTGAACGATACCGTGCCACGTTTc < 1:70866/251‑1 (MQ=255) tGGCGCTGGGGTTAAACTCAGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGGTTTTTT‑TCTGCGGCCCCAGTGAACGATACCGTGCCACGGTTCCTGCACCACgggc > 1:125943/1‑247 (MQ=255) atcTCGTTTAACAGCGGCCGTTTGCTGTCGCAGATGTGCTAGTACGTGGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTAGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTGTATT‑GCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAg < 2:24033/247‑1 (MQ=255) cGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATT‑GCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGAc < 1:3104/250‑1 (MQ=255) tCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTATACTGGCGAAATCACTCACGCTGCCGTGGAAAAGTTTTATTTGC‑GGGACCAACGTGAACAATACCGTGCCCCCATTACTGCACCACGGTCCGTGGGCACCCAAACCGACTCAGGCGCCGTTATCCAAc > 1:183573/1‑251 (MQ=255) actTTGAAGCAGTTGCGCAGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATT‑GCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACt < 1:10787/249‑1 (MQ=255) tgggcaccTGCCGCAGTAAGCGCGCAACCAGCTGTTTGTCTCGGGGCCAAGCGGAAACTTCGGCGATTTGACGGCTGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATT‑GCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGGCAGCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCTTGTGGaa < 2:180626/243‑1 (MQ=255) aGCTGCCGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTTCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATT‑TCTTCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCCAAGCGACTCAGGCGACGTTATCCAACGCGCTTGACGTGAGT‑CAGCCCAACAACTGGCCGCGTGTGGAagaggtg > 1:140074/1‑248 (MQ=255) gCCGCAGGAAGCGCTCAACCAGCTGGTTGTCTCGGTGCCAAGCTGAAACTTCGGCGATTTGACGGCGGGTCTGCTAGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATT‑GCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGTTCAGTGGTCACCCAAAGCTACTCAGGCGACGTTATCCAACGCGGTGGGCGGGAGG‑CAGCCGAGCAACTGGCCGCGTGTTGAAGAtttgtt < 2:111494/249‑1 (MQ=255) gCCGCAGGAAGCGCTCAACCAGCTGGTTGGCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATT‑GCTGCGACCAACGGGAACGATACCGTGCCACGTTTCCGGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGGGGAAGAgttgtt < 2:227322/249‑1 (MQ=255) cGCAGGAAGCGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGGGAAACGTTTTATT‑GCTGCGACCAACGTGAACGGAACCGTGCCACGTTTCCTGCCCGACGGTCAGTGGTCCCCCAAAGCGACACAGGCAACGTTATCCCACGCGATGGACGGGAGTTCA‑CCCAACCAATGGCCGCGGGTGGAAAGGTTGTTccgc > 2:213681/1‑251 (MQ=255) || GGCAAAATCAGTCCACTGCAAGAAAAACTGTTCTGTACATTGGGCGGCAATATCGAAACTGTTGCCATCGACGGCGATTTCGATGCCTGTCAGGCGCTGGTGAAGCAGGCGTTTGATGATGAAGAACTGAAAGTGGCGCTAGGGTTAAACTCGGCTAACTCGATTAACATCAGCCGTTTGCTGGCGCAGATTTGCTACTACTTTGAAGCTGTTGCGCAGCTGCCGCAGGAGACGCGCAACCAGCTGGTTGTCTCGGTGCCAAGCGGAAACTTCGGCGATTTGACGGCGGGTCTGCTGGCGAAGTCACTCGGTCTGCCGGTGAAACGTTTTATT‑GCTGCGACCAACGTGAACGATACCGTGCCACGTTTCCTGCACGACGGTCAGTGGTCACCCAAAGCGACTCAGGCGACGTTATCCAACGCGATGGACGTGAGT‑CAGCCGAACAACTGGCCGCGTGTGGAAGAGTTGTTCCGC > NC_000913/4211‑4684 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |