Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,555,047	2 bp→TG	coding (1963‑1964/2706 nt)	malT →	DNA‑binding transcriptional activator MalT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,555,047	0	G	T	94.7%	97.8 / NA	38	V655C (GTC→TGC)	malT	DNA‑binding transcriptional activator MalT
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (15/21);  total (15/23)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NC_000913	3,555,048	0	T	G	100.0%	108.9 / NA	38	V655C (GTC→TGC)	malT	DNA‑binding transcriptional activator MalT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/23);  total (15/23)

ATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAACCTGTTGCTG  >  NC_000913/3554824‑3555283                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                            aTGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCg                                                                                                                                                                                                                   <  1:177382/251‑1 (MQ=255)             gcgGCTGGATGACACCGAATAGTCGGCGCGGAGCGGGATTGACGTCATGCCGTCGTATCAGCCACAGTAACAGCTTAAGTGCCTGGCAATGTGGATGCTATGCTTGATGGCCTGTGGTG‑ATTTATATAACGCCCGTAGCCAGTTGAACCGTCTGGAAAACCCGCTGGGGAATGGCAAATATCACAGCGACTTGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGTCAAATGACCGGCTATAAAgccg                                                                                                                                                                                                       <  2:148298/251‑1 (MQ=255)                             gAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGtt                                                                                                                                                                                       <  1:61336/251‑1 (MQ=255)                               aGCGTCGGCGCGTAGCGGGATGGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGCCAGGTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTTGATCTCGAACGCCAACAAATGCCGGGGGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGc                                                                                                                                                                                     <  1:182788/251‑1 (MQ=255)                                         cgTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGc                                                                                                                                                                           >  2:203716/1‑251 (MQ=255)                                            aGCGGGATGGAAGTCTTGTCGGCTTATCAGCCACAGCAACAGCGTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCGAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTaa                                                                                                                                                                        <  1:158926/251‑1 (MQ=255)                                                 gATTGAAGTCTTGTCGTCTTATCAGCCACAGCACCAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAAcc                                                                                                                                                                     >  2:82233/1‑249 (MQ=255)                                                  aTTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCaga                                                                                                                                                                  <  2:127782/251‑1 (MQ=255)                                                   ttggagcgtagtcgtcTTATCAGCCACAGCAACAGCTTCAGTGACTGGCACTGTTGATTGAATGCTCGCTGCCCCGTGGAG‑ATTTAGATACAGCACGTAGCCAGCTGAACCCTCTGGAAAACCTGCTGGGGAATCGCAAATATCACAGCGCCTGGATCTCTAACGCCAACATATGCCGGAAGATTTACTGCCAAATGCCCGGCGATCAAGCCGCCGCTGCCAACTGGCTGCGTCATACGGAAAAACCag                                                                                                                                                                   >  2:33637/11‑249 (MQ=255)                                                       aGTCTTGTCGTCTTATCAGCCACAGCAACAGCTCCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGTTCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCACATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAAAGTTTg                                                                                                                                                             >  2:198950/1‑251 (MQ=255)                                                             gtcgtcTTATCATCCACAGCAAAAGTTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACAAGAGTTTGCGaaca                                                                                                                                                       <  2:153314/251‑1 (MQ=255)                                                                      tCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTcc                                                                                                                                              >  1:52685/1‑251 (MQ=255)                                                                           cacaGCAACAGTTTTAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCaa                                                                                                                                         <  2:203866/251‑1 (MQ=255)                                                                                  aCAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAAt                                                                                                                                  >  1:140509/1‑251 (MQ=255)                                                                                     gCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATCAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGgc                                                                                                                               >  2:35813/1‑251 (MQ=255)                                                                                             gCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACAtt                                                                                                                       <  1:153015/251‑1 (MQ=255)                                                                                                           aTTCAATGCGCGCTGGCCCG‑GGTGGATTTAGATACCGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCGAGGGAATGGCAAATATCCCAGCGACTGGATGTGTAACGCCAACAAAGGCCGGGTGATTTCCTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTTCGTCATACGGCTAATGCAGAGTTTGCTAACAGCCACTTCCTTCTAGGTCAATGGCTCAACAGTCCCCGTGCACAAAt                                                                                                         <  2:246343/251‑1 (MQ=255)                                                                                                            ttCAATGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGTCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATc                                                                                                        >  2:198909/1‑251 (MQ=255)                                                                                                              cAATGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCtt                                                                                                      <  1:203716/251‑1 (MQ=255)                                                                                                               aaTGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGACAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCCAGGTCAATGGCGAAACATTGCCCGTGCACAAATCTTg                                                                                                     >  2:206393/1‑251 (MQ=255)                                                                                                                aTGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAATTCTTGc                                                                                                    >  1:99105/1‑251 (MQ=255)                                                                                                                            ccGTGGTG‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTg                                                                                        <  2:128611/251‑1 (MQ=255)                                                                                                                              gtggtg‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCACCAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCCGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGa                                                                                       >  2:123121/1‑250 (MQ=255)                                                                                                                                   g‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGTGCGATTTTGAACCGGc                                                                                 <  2:232573/251‑1 (MQ=255)                                                                                                                                        tAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGa                                                                              >  1:211136/1‑250 (MQ=255)                                                                                                                                                 cccGTAGCCAGCGGACCCTTCTGGAAAACCTGCGGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGGCCGGGTGATTGACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAAACTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTtctc                                                                    <  2:248583/251‑1 (MQ=255)                                                                                                                                                   cGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCga                                                                  <  2:66356/251‑1 (MQ=255)                                                                                                                                                               aaCCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCGGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGa                                                      <  2:140509/251‑1 (MQ=255)                                                                                                                                                               aaCCGTATGGAAAACCTGCTGGGGAATGGCCAATATCACAGCGACTGGATCTCTAACGCAAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATg                                                       >  2:229866/1‑250 (MQ=255)                                                                                                                                                                       ggAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGccc                                              <  1:82233/251‑1 (MQ=255)                                                                                                                                                                           aaCCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAg                                          <  1:59483/251‑1 (MQ=255)                                                                                                                                                                                                 atCACAGCGACTGGAGCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGAtct                     <  2:52685/250‑1 (MQ=255)                                                                                                                                                                                                  tCACAGAGACTGGATCTCTAACGCCAACAACTGCCGGGTGATTTACTGGCAAATGACCGGCGCTAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGAtctc                    >  2:191342/1‑250 (MQ=255)                                                                                                                                                                                                    acaGCGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAAc                 <  1:255210/251‑1 (MQ=255)                                                                                                                                                                                                        cGACTGGATCTCTAACGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATAAGGCTAAACCAGAGTTTGCGAACAACCACTTACTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGt              >  1:260318/1‑250 (MQ=255)                                                                                                                                                                                                            gggATCTCTAACGACAACAAATGCCGGGTGATTTACTGTCAAATGACCGGCGATAAAGCCTCCGCTGCCAACTGGTTGCGTCATACGGCTAAATCAGTGTTTGCGAACAACCACTTCCTTCAAGGTCAATTGCGCAACATTGCCCGTGCACAAATCGTGCTGGGCTAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAGAATGCCCGGAGGCTGCGGTTGATGAGCGATCTCAACCGTAACCt         <  1:22400/250‑1 (MQ=255)                                                                                                                                                                                                                     aaCGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAACCTGTtgctg  <  1:212332/249‑1 (MQ=255)                                                                                                                                                                                                                     aaCGCCAACAAATGCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAACCTGTtgct   <  1:191342/248‑1 (MQ=255)                                                                                                                                                                                                                                 ||                                                                                                                                                                                                                                            ATGGGCCTGGGCGCGGCTGGATGAAGCCGAAGCGTCGGCGCGTAGCGGGATTGAAGTCTTGTCGTCTTATCAGCCACAGCAACAGCTTCAGTGCCTGGCAATGTTGATTCAATGCTCGCTGGCCCGTGGTG‑ATTTAGATAACGCCCGTAGCCAGCTGAACCGTCTGGAAAACCTGCTGGGGAATGGCAAATATCACAGCGACTGGATCTCTAACGCCAACAAAGTCCGGGTGATTTACTGGCAAATGACCGGCGATAAAGCCGCCGCTGCCAACTGGTTGCGTCATACGGCTAAACCAGAGTTTGCGAACAACCACTTCCTGCAAGGTCAATGGCGCAACATTGCCCGTGCACAAATCTTGCTGGGCGAGTTTGAACCGGCAGAAATTGTTCTCGAAGAACTCAATGAAAATGCCCGGAGTCTGCGGTTGATGAGCGATCTCAACCGTAACCTGTTGCTG  >  NC_000913/3554824‑3555283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑