| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,497,385 | 4 bp→CAA | coding (530‑533/1656 nt) | opgD → | glucans biosynthesis protein D |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,497,385 | 0 | T | C | 87.5% | 36.2 / ‑4.3 | 16 | F177S (TTT→TCT) | opgD | glucans biosynthesis protein D |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (8/6); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 1,497,386 | 0 | T | . | 93.3% | 51.6 / ‑0.7 | 16 | coding (531/1656 nt) | opgD | glucans biosynthesis protein D |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (8/6); minor base A (0/1); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 1,497,387 | 0 | C | A | 100.0% | 41.4 / NA | 16 | L178N (CTC→AAC) | opgD | glucans biosynthesis protein D |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (9/7); total (9/7) | |||||||||||
| * | NC_000913 | 1,497,388 | 0 | T | A | 100.0% | 44.4 / NA | 16 | L178N (CTC→AAC) | opgD | glucans biosynthesis protein D |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (9/7); total (9/7) | |||||||||||
AATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATTG > NC_000913/1497182‑1497604 ||| aatgggaTTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTGAAAGCCTCCGAACTGGCGTGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACgg < 1:242787/251‑1 (MQ=255) cgccgcGTTCGTATTTTTGCTGGAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATAGCAATACGTTTTTTCGGCCcg < 2:233755/250‑1 (MQ=255) gTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCg > 1:92364/1‑251 (MQ=255) tttCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATGTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGCTGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTCCACCCACAGTAAAGAAGAGTTCCCCGAAtttccaccc > 2:175429/1‑245 (MQ=255) gAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGttt > 1:54550/1‑251 (MQ=255) gtggATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTGGCCGGGTTTCGCGTGTTTAAAGCCCCCGAACTGTCGCGCCGTGATTAAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGGTGATGATACATATCAATACGGTTTGGCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACGTTACCGCCCTCTGGTGTGATACGGTAAAACCGGGTGCATCTAcc < 2:225699/248‑1 (MQ=255) gagaCAAAACAATGTGAAGGGCAAAGGGTGCTCGGCTTTGGCGGTTTTCGGGTGTTTAAAGCCCCCGACCTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGTGGGTTATTTCCGCGCCGTGGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTGACACCGGCAGTAAAGAAGAGTTCCCCGACTTTACCGCTTTCTGGTTTGATACGGTAAAACCGGGGGCAACTTCCttt < 2:20119/248‑1 (MQ=255) gATACAACGCAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTACAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGCGTTATTTCCGCGCCGTTGATGATACATCTCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGCCAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCttt > 2:34887/1‑251 (MQ=255) aCAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGttt > 1:217245/1‑250 (MQ=255) aGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGcc < 1:84057/195‑1 (MQ=255) aGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGcc > 2:84057/1‑195 (MQ=255) gggaaaaGCCATCTGGGCTTTGCCGGTTTTGGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGATTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTc < 1:37736/247‑1 (MQ=255) agcgagttcGGCTTGGCCGGTTTTCGCGTTTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTAT‑AATAAACGGCGCGAGTTATTGCCGCGCCGTTGATGATACATATCAATACGGTTTGGCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTGTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCGCGATAgc < 2:197359/244‑1 (MQ=255) ggTTTTCGCGTGTTTAAAGCCCCCGAACTTGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGCGCGACACTTACACCGACAGTACAGAAGAGTTCCTCGACTTTACCGCCTTCTGGTTTGATACGGCAAAACCGGGCGCAACTACCTTTACCGTTTATGCGTCGCTCGATCACGCCAGCATTACTGGCGcc > 1:166438/1‑251 (MQ=255) tgtTTAAAGCCCCCGAACTGGCGAGCCGTGATGTAGTATC‑CTCAACGGTGCGAGTTATTTCCGCGACGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACAATTACACCGCCAGTACAGAAGACTTCACCGACTTTAACGCCTTCTGGTTTGATAAGGTCAAACCGGGGGCAACTACCTTTACCGTTTTTGCGTTGCTAGATAGAGCACGCACTACTGGTGCCTATAAGTTc > 1:21015/1‑250 (MQ=255) ccccGAACTGGCGCGCCGTGATGTAGTATCATC‑AACGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACCCTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATtg > 2:103088/1‑251 (MQ=255) ||| AATGGGATTCCGTCGCCGCGTTCGTATGTTTTCTGTAGATCCAGCAACACATCTGGCGCGTGAAATTCACTTTCGCCCGGAGTTGTTCAAATACAACGATGCAGGTGTTGATACAAAACAATTAGAAGGGCAAAGCGATCTCGGCTTTGCCGGTTTTCGCGTGTTTAAAGCCCCCGAACTGGCGCGCCGTGATGTAGTATCATTTCTCGGCGCGAGTTATTTCCGCGCCGTTGATGATACATATCAATACGGTTTGTCGGCCCGCGGCCTGGCGATCGACACTTACACCGACAGTAAAGAAGAGTTCCCCGACTTTACCGCCTTCTGGTTTGATACGGTAAAACCGGGGGCAACTACCTTTACCGTTTATGCGTTGCTCGATAGCGCCAGCATTACTGGTGCCTATAAGTTCACTATCCATTG > NC_000913/1497182‑1497604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |