Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,331,059	2 bp→AT	coding (12‑13/2598 nt)	topA →	DNA topoisomerase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,331,059	0	T	A	100.0%	70.3 / NA	23	A4A (GCT→GCA)	topA	DNA topoisomerase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/9);  total (14/9)
*	NC_000913	1,331,060	0	C	T	100.0%	67.3 / NA	23	L5F (CTT→TTT)	topA	DNA topoisomerase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/9);  total (14/9)

GCTTTCGTCAATCTGGCAATAGATTTGCTTGACATTCGACCAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATC‑TGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCAC‑ATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTG  >  NC_000913/1330818‑1331274                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                         gCTTTCGTCAATCTGGCAATAGATTTGCTTGACATTCGACCAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATc                                                                                                                                                                                                                  >  1:191858/1‑251 (MQ=255)                                                       gCTATAGCGCCTGTAGGCCAAGACCTGTTATCTCAGTCTCCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATAGCCGCAGAGAGCGAGTCCATCTCGGTAACTCGTTGCCAGTGGAAGGGTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTCAGGTGAATATGGGTAAAGCATGTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAGCAAGTATC‑TGGGTCGTtactac                                                                                                                                                           <  2:191591/251‑1 (MQ=255)                                                       gCTATAGCGCCTGTAGGCCAAGACCGGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATC‑TGGGTAGTGACTAc                                                                                                                                                           <  1:161613/251‑1 (MQ=255)                                                                         cAAGAACTGTTAACTAAGTCACCTGAATTTTCGTGAACAGAGTCACGCCAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGACAGTGACAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATC‑TGGGTAGTGACTACGTGGTGAAATCCAGCGTc                                                                                                                                         >  2:159579/1‑251 (MQ=255)                                                                           aGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATC‑TGGGt                                                                                                                                                                    <  1:123036/222‑1 (MQ=255)                                                                           aGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATC‑TGGGt                                                                                                                                                                    >  2:123036/1‑222 (MQ=255)                                                                                          gTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGATTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATC‑TGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCCC‑ATCCGCGAttt                                                                                                                       <  1:93127/251‑1 (MQ=255)                                                                                                tGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTAATCGTTGAGTCACCGGCAAAAGCCAAAACGATCACCAAGTATC‑TGGGTAGTGACTCCGTGGTGAAATCCAGCGTCGG‑CACCATCCACGATTTGCCGAc                                                                                                                 >  2:99027/1‑251 (MQ=255)                                                                                                                 agTCAAGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATC‑TGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCAC‑ATCCGCGATTTGCCGACCAGTGGCTCAGCTGcc                                                                                                 >  2:199593/1‑250 (MQ=255)                                                                                                                   tCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAAAGTGCAACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGCATATGGGCAAAGCATTTGTCATCGTTGCGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATC‑TGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCAC‑ATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaa                                                                                               >  1:246608/1‑250 (MQ=255)                                                                                                                       gACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATC‑TGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCAC‑ATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCaaaa                                                                                             >  1:254664/1‑248 (MQ=255)                                                                                                                               gTTGATATCCGCAGAGAGCGAGTCCATCTCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATC‑TGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCAC‑ATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCCAACAGAGTg                                                                                       >  2:118753/1‑246 (MQ=255)                                                                                                                               gTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATC‑TGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCAC‑ATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTg                                                                                       <  1:118753/246‑1 (MQ=255)                                                                                                                                  gATATCCGCAGAGTGCGAGCCCATAACGGTAACTCGTTGCCAGTGGAAGGGTTATCAACGTTCGACGCATGCCTGGAAGAATCAAATTAGGTAAGGTGAATATTGGTAACGTATTTGTTATCGTTGAGTCCCCGGCAAAAGACAAAACGGTGAACAAGTATCGTGGG‑AGGGACTACGTGGTGAAAGCCAGCGTGGGTCAC‑ATCCGCGATCTGCCGCCCAGTGGCTCAGCTGTCAAAAAGAGTGTCCActct                                                                               <  2:264991/251‑1 (MQ=255)                                                                                                                                         gCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCACATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATC‑TGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCAC‑ATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTctccctcc                                                                         >  2:84397/1‑250 (MQ=255)                                                                                                                                                 cGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATC‑TGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCAC‑ATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACg                                                                >  2:210624/1‑251 (MQ=255)                                                                                                                                                   aGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATC‑TGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCAC‑ATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGc                                                              >  1:42518/1‑251 (MQ=255)                                                                                                                                                                       tGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATC‑TGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCAC‑ATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGAt                                           <  1:144875/250‑1 (MQ=255)                                                                                                                                                                                             gTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATC‑TGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCAC‑ATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCg                    <  1:99477/251‑1 (MQ=255)                                                                                                                                                                                                cGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATC‑TGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCAC‑ATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGATTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTAt                 >  1:214354/1‑251 (MQ=255)                                                                                                                                                                                                  acgcatacgtgggggagtcaacttgggTAAGGTGAATATGGGCAAACCATTTGTCATAGTTAAGTCCCCGGCAAAAGCCAAAACGCTCAAAAAGTATC‑TGGGTAGTGACTACGTGGCGCAATCCAGCGCCGGTCAC‑ATCCGCGATTTGCCGACAAGTGACTCAGCAGCCCAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTCAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATgg               >  2:122345/26‑251 (MQ=255)                                                                                                                                                                                                           tggcacaaacAAATTAGGTAAGGCGAATATGGTTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGCTCAACAAGTATC‑TGGGTAGTGACTACGTGGTGAAATACAGCGTCGGTCAC‑ATCCGAGATTTGCCGACCAGTGGCTAAGCTGCCATAAAGAATGCCGCCTATACCTCCACCAGGACGGCTAAAACGCCTCAAAAGGATGCACGTGCCGCTCTACTCCACCGTCTGGTGGTTGAcc      >  2:156709/10‑251 (MQ=255)                                                                                                                                                                                                               agaaTCAAATTAGGTAAGGTGAATATGGGTAAAGCATTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATC‑TGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCAC‑ATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTg  <  2:75329/251‑1 (MQ=255)                                                                                                                                                                                                                                                  ||                                                                                                                                                                                                                         GCTTTCGTCAATCTGGCAATAGATTTGCTTGACATTCGACCAAAATTCCGTCGTGCTATAGCGCCTGTAGGCCAAGACCTGTTAACTCAGTCACCTGAATTTTCGTGAACAGAGTCACGACAAGGGGTTGATATCCGCAGAGAGCGAGTCCATATCGGTAACTCGTTGCCAGTGGAAGGTTTATCAACGTGCGACGCATTCCTGGAAGAATCAAATTAGGTAAGGTGAATATGGGTAAAGCTCTTGTCATCGTTGAGTCCCCGGCAAAAGCCAAAACGATCAACAAGTATC‑TGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCAC‑ATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTG  >  NC_000913/1330818‑1331274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑