Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	17,228	2 bp→AT	intergenic (+218/‑260)	sokC → / → nhaA	small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	17,228	0	G	A	100.0%	28.6 / NA	10	intergenic (+218/‑261)	sokC/nhaA	small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/2);  total (8/2)
*	NC_000913	17,229	0	C	T	100.0%	26.5 / NA	10	intergenic (+219/‑260)	sokC/nhaA	small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/2);  total (8/2)

TCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAA‑CGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTA  >  NC_000913/16994‑17446                                                                                                                                                                                                                                           ||                                                                                                                                                                                                                            tCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGCTAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTACCTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTAaca                                                                                                                                                                                                               >  1:24472/1‑250 (MQ=255)                                         acagatagCCTCAAGCACCCGCCGCTCTTATATCGCTCTCTTTAAACAATTCTGTTTTATCGCTTCTCATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTCTTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAAt                                                                                                                                                                      >  2:116379/1‑251 (MQ=255)                                                                         tCGCTCTCTTTAACCCATTCTGTTTTATCGATTCTAATCCTGACGACGCCCTGCATTTTTGTGGCGTAATTTTTTAATGATTTAACTCTTTAACTTTAAATTACCCCTTTATCGCAATTATTGCCGACACGTTGGATTATTTTTGTAATATTGTCCTAACAAATAACGCCGACTGACAAGAACTTAATTATTACTTTTCCTAATTAATCCCTCAGCAATCCTCACCTTAAGCTATGATTATCTGGGCtt                                                                                                                                        >  1:240815/1‑249 (MQ=255)                                                                              ctccttAACCCATTCGGTTTTATCGATTTTAATTCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATTATTTAATTATTTAACTTGAATTTATCTCTTTATCTCAATTATTGACGACAAGCTGGATTATTTGTGAAATATTGTCCTAACAAATAACTCCGACTGACAAGAAATTAAGTATTTCTTGTCCGAATTAAGCCCGCAGAAATCCTCACCTTAAGCTATCATGATCTGTGCTTAGGGt                                                                                                                                   <  2:246832/245‑1 (MQ=255)                                                                                                                cTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCACTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGTGCTTACAGCCGTCa‑aaaaactcatc                                                                                              >  1:88609/1‑244 (MQ=255)                                                                                                                                                  ttAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATCATTGACGACAAGCTGAATTATTTTTGAAATATTGTCATTACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCTGAAATCCTCACCTTAAGCTAAGATTATCTGGGATTCGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGTTGGCGAAAATTCTTCattggatcg                                                            >  1:248674/1‑243 (MQ=255)                                                                                                                                                                                tctcTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGATATTCCTACACTATAATc                              >  1:193165/1‑251 (MQ=255)                                                                                                                                                                                           cAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACg                   <  1:182297/251‑1 (MQ=255)                                                                                                                                                                                                     cgacAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTcc                                          >  1:15264/1‑218 (MQ=255)                                                                                                                                                                                                             tGGATTATTTTTGGAATATTGTCCTAACAAATAACGACGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCACAAAA‑CTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTACTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTa  >  2:96204/1‑250 (MQ=255)                                                                                                                                                                                                                                           ||                                                                                                                                                                                                                            TCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCA‑AAAA‑CGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTA  >  NC_000913/16994‑17446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑