Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,591	C→T	V195V (GTC→GTT)	creB →	DNA‑binding transcriptional regulator CreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,591	0	C	T	100.0%	82.6 / NA	28	V195V (GTC→GTT)	creB	DNA‑binding transcriptional regulator CreB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/15);  total (13/15)

AGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAA  >  NC_000913/4636346‑4636834                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    aGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATAc                                                                                                                                                                                                                                                >  1:264928/1‑251 (MQ=255)      gctcACCTTACTGCGTCGGGGGAAGAAGTTCGCGTCGCCGTCCCCCGACACCCTTATTGGTCATTTGGAATTGAATGAACCCGGGGCGCAGATCAGCTGGTTTGCCACGCCATTAACGCTGACGCGGTATGGGGTTTTATTGCTGAAGACGTTCCTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCcaca                                                                                                                                                                                                                                           <  1:84893/248‑1 (MQ=255)      gcgcACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACCCCTACGATCGCACCGTTGATAccccc                                                                                                                                                                                                                                            >  2:215005/1‑248 (MQ=255)              tACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACAAGCCATTAACGCTGCCGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTCAAACg                                                                                                                                                                                                                                   >  1:114376/1‑251 (MQ=255)                      ggggTGAAGAAGTTCTCGTCGCCGCCCCCCGGAATCCGTATGGGTCATTTGGAATTGAATAACCCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGc                                                                                                                                                                                                                           <  1:147149/250‑1 (MQ=255)                                                                    atttggatttgaacaaaCCCGCGGCTCATATTAGTTGTTGTGACACGCCATTACCGCTTACGCGTTTTGATTTTTTATTGCTGAACACGTTACTCAGGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCCTTGATCCCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATTCTCAt                                                                                                                                                                             <  2:69122/236‑1 (MQ=255)                                                                            ttgaatGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCAt                                                                                                                                                                     >  1:44931/1‑251 (MQ=255)                                                                                              cAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCATCTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAgggg                                                                                                                                                   >  2:70297/1‑251 (MQ=255)                                                                                               aGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGc                                                                                                                                                  >  2:246974/1‑251 (MQ=255)                                                                                                       tGGTTTGACACGCCATAAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGCTGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGAGGCCTTTAAt                                                                                                                                           >  2:65066/1‑250 (MQ=255)                                                                                                                                      aTGAGTTTTTCTTGCTGAAGACGATACTCAAGTCGCCAGGCCGCGTAAGGTCCCGACAGCAACTAATGGATAGCGTGTGGGACGATGCGCAGGACACCTACGATCGCACCGATGATACCCACATTCAAACGTTGAGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAtt                                                                                                           >  2:115606/1‑251 (MQ=255)                                                                                                                                      aTGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGGTGCTGCTGGGCTAtt                                                                                                           >  2:210142/1‑251 (MQ=255)                                                                                                                                              ttATTGCTGAAGACGTTCCTCAAGTCGCCAGGCCTGGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACt                                                                                                    <  2:264928/250‑1 (MQ=255)                                                                                                                                                            tttACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAAcccc                                                                                                                                                                                                     <  2:223811/138‑1 (MQ=255)                                                                                                                                                            gTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAAcccc                                                                                                                                                                                                     >  1:223811/1‑139 (MQ=255)                                                                                                                                                                  caaggccgcaggccgcgactattCCCGCCAGCAACTAATGGATAGCGATTGGGAAAATGCGCAGGACACCTACGATCGCACCGTTGATACGTACATTAATACGTTGCGAGCAACGCTGCGCGCCATCAACTCCGAGCTGTCACCGAGTAAGTGTCTTCGCGGTATGGTTTATAGCCGGAGGGGCCTTTAATGCGTAGCGGTATGGGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTgg                                                                                 <  1:252534/227‑1 (MQ=255)                                                                                                                                                                  cAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATCCCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGtt                                                                               <  1:215005/251‑1 (MQ=255)                                                                                                                                                                    agtcgccaggCCGCGGTTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCg                                                                             <  1:219079/243‑1 (MQ=255)                                                                                                                                                                                                      aaTGGATAGCGTGTGGGAAGATGCGCAGGACCCCTACGATCGCATCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCgg                                            <  1:62404/249‑1 (MQ=255)                                                                                                                                                                                                      aaTGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCgg                                            <  1:70297/249‑1 (MQ=255)                                                                                                                                                                                                         ggATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATg                                                                                                                              >  2:42943/1‑165 (MQ=255)                                                                                                                                                                                                         ggATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATg                                                                                                                              <  1:42943/165‑1 (MQ=255)                                                                                                                                                                                                                                  gACACCTACGATCGCACAGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGtt                >  1:189116/1‑250 (MQ=255)                                                                                                                                                                                                                                         aCGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGat         <  1:234355/250‑2 (MQ=255)                                                                                                                                                                                                                                          cgagcGCACCGTTGATACCCACATTAAAACGGTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGTGGACGTTAATCGatac       <  1:204436/247‑4 (MQ=255)                                                                                                                                                                                                                                          cGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGatac       <  1:94127/251‑4 (MQ=255)                                                                                                                                                                                                                                              cGCACCGTTGATACCCACATTAAAACGTTGCTTTCCAATCTGCCCGCCATCAACTCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGTTATAGCCTTAGGGGCCTGTAATGCGTATCGGCATGCGGTTTCTGCTGGGCTATTTTTTGCTGGTGGCGGTTGCGGCCTTGTTCGTACTGGCTATGTTTGTCAAATAAGTTAAACCGGGCGTGCGAAGACCAACGGAGGGGACGttaagctataccgca   <  2:160967/251‑14 (MQ=255)                                                                                                                                                                                                                                                cACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGTTCGTACTGGCTATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTAATCGATACCGCaa  >  2:144197/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    AGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAA  >  NC_000913/4636346‑4636834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑