Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,516	G→A	P170P (CCG→CCA)	creB →	DNA‑binding transcriptional regulator CreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,516	0	G	A	96.4%	73.1 / ‑6.7	28	P170P (CCG→CCA)	creB	DNA‑binding transcriptional regulator CreB
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (17/10);  total (17/11)
Fisher's exact test for biased strand distribution p-value = 3.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

CGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTT  >  NC_000913/4636274‑4636757                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  cGCCTGCTTGGGCTGGACATTGGTGCTGACGCCTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCAAGGCCGCGt                                                                                                                                                                                                                                           >  2:94127/1‑251 (MQ=255)          gggCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAGGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCg                                                                                                                                                                                                                                  >  2:84893/1‑251 (MQ=255)            gCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGGGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGcc                                                                                                                                                                                                                                <  1:94694/251‑1 (MQ=255)                gAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGCAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGc                                                                                                                                                                                                                             >  2:219079/1‑250 (MQ=255)                            gacgacTATGTGGCTAAACCGTTTTCCCCCGGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAAAAGTTCTCGTCGCCGTCTCCCGTCACCCGTATTGGTCATTTTGAATTGAATGAACCCGGGGCGCAGAGCAGCTGTTTTGACACGCCATTAGCGCTGACGCGGTATGTGTTTTTATTGCTGAAGTCGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGAt                                                                                                                                                                                                                  <  1:113839/249‑1 (MQ=255)                                   atgTGGCTAAACCGTTTTCACCCCGCGAAGTGGGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTACCGCTGACGCGGGATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTgg                                                                                                                                                                                                         >  1:216935/3‑251 (MQ=255)                                      tGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGa                                                                                                                                                                                                       >  1:77076/1‑250 (MQ=255)                                                     cACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTAAAGAAGTTCTCGTCGCCGTCCCCCGTCATCCGTATTGGTCATTTTGAATTGAATGACCCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTGCTCAAGTCGCCAGGCCGCGTCTGGGCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACAcc                                                                                                                                                                                       <  1:160178/251‑1 (MQ=255)                                                             gaggtgggcgcCGGGGTCCGCCCCTTACTGCTGCGGGTGAAGAATTTCTCGTCGCCGTCCCCCGTCACCCTTATTGGTCATTTTGAATTGAATGACCCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCGGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCg                                                                                                                                                                               <  2:207501/244‑1 (MQ=255)                                                               aGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGCCGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGc                                                                                                                                                                              >  2:234355/1‑250 (MQ=255)                                                                gtgtGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCAc                                                                                                                                                                            >  2:126389/1‑251 (MQ=255)                                                                 ggtgCGCCGGGGTCCGCACTTTACGGCGTGGGGGGAAGAAGTTCGCGTCGCCGTCCCCCGTCTTCCGTTTTGGTCATTTGGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACTTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCAcc                                                                                                                                                                           <  1:179217/250‑1 (MQ=255)                                                                         aGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATAc                                                                                                                                                                   >  1:264928/1‑251 (MQ=255)                                                                              gctcACCTTACTGCGTCGGGGGAAGAAGTTCGCGTCGCCGTCCCCCGACACCCTTATTGGTCATTTGGAATTGAATGAACCCGGGGCGCAGATCAGCTGGTTTGCCACGCCATTAACGCTGACGCGGTATGGGGTTTTATTGCTGAAGACGTTCCTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCcaca                                                                                                                                                              <  1:84893/248‑1 (MQ=255)                                                                              gcgcACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACCCCTACGATCGCACCGTTGATAccccc                                                                                                                                                               >  2:215005/1‑248 (MQ=255)                                                                                      tACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACAAGCCATTAACGCTGCCGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTCAAACg                                                                                                                                                      >  1:114376/1‑251 (MQ=255)                                                                                              ggggTGAAGAAGTTCTCGTCGCCGCCCCCCGGAATCCGTATGGGTCATTTGGAATTGAATAACCCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGc                                                                                                                                              <  1:147149/250‑1 (MQ=255)                                                                                                                                            atttggatttgaacaaaCCCGCGGCTCATATTAGTTGTTGTGACACGCCATTACCGCTTACGCGTTTTGATTTTTTATTGCTGAACACGTTACTCAGGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCCTTGATCCCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATTCTCAt                                                                                                <  2:69122/236‑1 (MQ=255)                                                                                                                                                    ttgaatGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCAt                                                                                        >  1:44931/1‑251 (MQ=255)                                                                                                                                                                      cAGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCATCTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAgggg                                                                      >  2:70297/1‑251 (MQ=255)                                                                                                                                                                       aGATCAGCTGGTTTGACACGCCATTAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGc                                                                     >  2:246974/1‑251 (MQ=255)                                                                                                                                                                               tGGTTTGACACGCCATAAACGCTGACGCGGTATGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGCTGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGAGGCCTTTAAt                                                              >  2:65066/1‑250 (MQ=255)                                                                                                                                                                                                              aTGAGTTTTTCTTGCTGAAGACGATACTCAAGTCGCCAGGCCGCGTAAGGTCCCGACAGCAACTAATGGATAGCGTGTGGGACGATGCGCAGGACACCTACGATCGCACCGATGATACCCACATTCAAACGTTGAGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTAtt                              >  2:115606/1‑251 (MQ=255)                                                                                                                                                                                                              aTGAGTTTTTATTGCTGAAGACGTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGGTGCTGCTGGGCTAtt                              >  2:210142/1‑251 (MQ=255)                                                                                                                                                                                                                      ttATTGCTGAAGACGTTCCTCAAGTCGCCAGGCCTGGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACt                       <  2:264928/250‑1 (MQ=255)                                                                                                                                                                                                                                    tttACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAAcccc                                                                                                                        <  2:223811/138‑1 (MQ=255)                                                                                                                                                                                                                                    gTTACTCAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATACCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAAcccc                                                                                                                        >  1:223811/1‑139 (MQ=255)                                                                                                                                                                                                                                          cAAGTCGCCAGGCCGCGTCTGGTCCCGCCAGCAACTAATGGATAGCGTGTGGGAAGATGCGCAGGACACCTACGATCGCACCGTTGATCCCCACATTAAAACGTTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGCTGCTGGGCTATTTTTTACTGGTGGCGGTGGCGGCCTGGtt  <  1:215005/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  CGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCAGATCAGCTGGTTTGACACGCCATTAGCGCTGACTCGGTATGAGTTTTTATTGTTGAAGACGTTACTCAAGTCACCGGGCCGCGTCTGGTCCCGCCAGCAACTGATGGATAGCGTATGGGAAGATGCGCAGGACACCTACGATCGCACCGTCGATACCCACATTAAAACGCTGCGTGCCAAGCTGCGCGCCATCAACCCCGATCTTTCACCGATTAATACTCATCGCGGCATGGGATATAGCCTGAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTT  >  NC_000913/4636274‑4636757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑