Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,180	G→T	P58P (CCG→CCT)	creB →	DNA‑binding transcriptional regulator CreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,180	0	G	T	100.0%	105.8 / NA	34	P58P (CCG→CCT)	creB	DNA‑binding transcriptional regulator CreB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/16);  total (18/16)

CGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGG‑TACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATT  >  NC_000913/4635948‑4636415                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                            cGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGaa                                                                                                                                                                                                                           <  1:268510/251‑1 (MQ=255)    aaaaCGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAAtta                                                                                                                                                                                                                        <  2:178967/251‑2 (MQ=255)      aaCGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGGTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAAttatg                                                                                                                                                                                                                      <  1:48352/251‑4 (MQ=255)              tAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGGCTACC‑GACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAg                                                                                                                                                                                                               <  1:128804/250‑1 (MQ=255)                gcgcGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTa                                                                                                                                                                                                            <  1:70398/251‑1 (MQ=255)                   cgGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTg                                                                                                                                                                                                         >  1:106416/1‑251 (MQ=255)                         cTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTATGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTc                                                                                                                                                                                                   >  1:25444/1‑251 (MQ=255)                          tGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTcc                                                                                                                                                                                                  >  1:49035/1‑251 (MQ=255)                           gTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGCTGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGAGCTcc                                                                                                                                                                                                  >  2:335/1‑250 (MQ=255)                                gCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCgg                                                                                                                                                                                            <  1:149482/251‑1 (MQ=255)                                         gcAATAACAGAGGCGGTTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATAAGCTAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGAAGG‑CTCCCGACGTCATGATTCTCGATGTTGTTTTGCCTGATATCGGCGGCTTTGAATTATGCCGCAAGTTACTGGCGCTCCATCCGGCGTTACCTg                                                                                                                                                                                   >  2:62404/1‑251 (MQ=255)                                         gcAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGCGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAACGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTg                                                                                                                                                                                   >  1:252618/1‑251 (MQ=255)                                                                aaCGGGAAAAGGTCTGGTTAGAGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAgg                                                                                                                                                            >  2:40168/1‑250 (MQ=255)                                                                             cTGGTTAGTGTAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGCCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGCTGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTACTGACTGCCCGGAGTGAAGAGGTCGATc                                                                                                                                               >  2:179217/1‑251 (MQ=255)                                                                                          gATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCt                                                                                                                                  <  1:215874/251‑1 (MQ=255)                                                                                                    ggATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGt                                                                                                                        <  2:49035/251‑1 (MQ=255)                                                                                                            gACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGCAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTgacg                                                                                                                 >  2:13100/1‑250 (MQ=255)                                                                                                            gACACGCTGGACTACATGTTGCAGCAGGAGGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTCCCGGCGCTGGATAAAGCTCGGCAGCAGG‑CTCCCGACGACATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGGCGCCAGGTACTGGCGCTCCATCCGGCGTTCCCTGTACTGTTCCTGACGGCCCGGCGGGAAGAGGGCGATCGCCTGATTGGTCTGGAAACTGGCGCTgacg                                                                                                                 >  2:143680/1‑250 (MQ=255)                                                                                                               acGCTGGTCTACATTTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTa                                                                                                             <  1:143816/251‑1 (MQ=255)                                                                                                                cGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTat                                                                                                            >  1:102045/1‑250 (MQ=255)                                                                                                                   tGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTAtgtg                                                                                                         >  2:147149/1‑251 (MQ=255)                                                                                                                                           gTTTTGCCGTCGAGGGCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCATG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATGATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTTTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGaa                                                                                 <  1:258271/251‑1 (MQ=255)                                                                                                                                             tttGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAgt                                                                               >  1:143676/1‑251 (MQ=255)                                                                                                                                                      gAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGTGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAg                                                                      <  1:212466/251‑1 (MQ=255)                                                                                                                                                                      gCTTGCCGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTg                                                      >  1:207501/1‑251 (MQ=255)                                                                                                                                                                            gggTGCTGGATAAAGCGCGCCAGTAGG‑CTCCCGACGTCATGATTCTTGATTTTGGTTTGCATGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCTCTACATGCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGACCGCCTGCTTGGGCTGGAAAGTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGTTGCGCCCCTTACTGCGTCgg                                                <  2:98944/250‑1 (MQ=255)                                                                                                                                                                            cGGTGCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCCGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGCAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTCAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGtagg                                                >  1:160967/1‑248 (MQ=255)                                                                                                                                                                                gCTGGATAAAGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGATTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGCGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGCCGACTATGTGGCTAAACCGTTTTCACCCCGCGTAGAGTGCGCCAGGGGGCGCACCTTACTGCGTCGGGTg                                             >  2:104228/1‑250 (MQ=255)                                                                                                                                                                                     aTAAAGCTCGCCAGCAGG‑CTCCCGACGACATGATTCTCGATGTTGGCTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTgaaga                                         >  2:23199/1‑249 (MQ=255)                                                                                                                                                                                         aGCTCGCCAGCAGG‑CTCCCGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTtct                                   <  1:335/251‑1 (MQ=255)                                                                                                                                                                                                          cccGACGTCATGATTCTCGATGTTGGTTTTCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTccc                     <  1:97479/249‑1 (MQ=255)                                                                                                                                                                                                          cccGACGTCATGATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTccc                     <  2:206207/249‑1 (MQ=255)                                                                                                                                                                                                                     gATTCTCGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTg        <  1:81760/251‑1 (MQ=255)                                                                                                                                                                                                                           cGATGTTGGTTTGCCTGATATCAGCGGCTTTGAATTATGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTATGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGTCGCCGTCTCCCGTCATCCGTATTGGTCAtt  >  2:94694/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                            CGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGG‑TACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATT  >  NC_000913/4635948‑4636415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑