Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,635,889	T→C	D123D (GAT→GAC)	creA →	PF05981 family protein CreA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,635,889	0	T	C	100.0%	53.8 / NA	20	D123D (GAT→GAC)	creA	PF05981 family protein CreA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/6);  total (14/6)

CCTTTGATGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACCGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGATGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGAT  >  NC_000913/4635651‑4636129                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 cctttgtcgatCCCGATGTGAAAAATGTCACCTGTTATGAGAGCCGCGCGCAAACAGGTGGTATTAAAGCAGGGTTGGGTCTAGCGAAAGATACATCCGATGCGGCTATTTCTTGTCAGCAAGTCGAGCCGATTGCACTGTCGGATCGTATTAAAAACGGCAACGCTCAGGGCGAGGAAGTATTCAAAACACGAACGACCCTGGTCTTTAAGTAGTTACAGGACGTGCGCTTTTATGCCGCCAaacgcaa                                                                                                                                                                                                                                       >  1:111856/9‑250 (MQ=255)               caTGTGAAAAATTTCACCTGTTATGTTAGCCGTTCGAAAACAGGTGGTATTAATGGGGGATTGGGTCTGGCGGAAGATTCCTCCGATGCTGCTATTTCGTGTCAGCCAGTCGGTCCGATTGAACTGTCGGATCGTATTAAATACGGCAAAGCTCATGTCGATGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTTCAGTTCGTGCGCTTTTATTACGTCAACCGCAGCGCGCTCGCTTATCt                                                                                                                                                                                                                        <  2:268456/250‑1 (MQ=255)                         aTGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTcc                                                                                                                                                                                                              >  2:10098/1‑251 (MQ=255)                                         gAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAg                                                                                                                                                                                              <  1:124395/251‑1 (MQ=255)                                                   aaaaCAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCACGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCgc                                                                                                                                                                                     >  2:13858/1‑249 (MQ=255)                                                                               gtcgggCGGAAGATGGCTCCGATGCGGCTATTGCTGGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTGAAGTCGTTACAGGTCGTGCGCTTTGATGTCGGCAGTCGCAACGCGCTCGCTGGTCTGGCTGAGGCCGACAAAGTTGCAGAAGGTACGCCGAAGAACGCGGTGAGCGCGGTGCCTTTCATg                                                                                                                                                        <  2:17482/247‑1 (MQ=255)                                                                                               cTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAAc                                                                                                                                        >  1:206207/1‑251 (MQ=255)                                                                                                                                  cGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTCTTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGAGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATATGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGCTTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGtt                                                                                                      >  1:235481/1‑250 (MQ=255)                                                                                                                                      tGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAAc                                                                                                               >  1:236873/1‑237 (MQ=255)                                                                                                                                      tGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAAc                                                                                                               <  2:236873/237‑1 (MQ=255)                                                                                                                                                    gTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGCCGATGAGCAAggg                                                                                   >  2:212466/1‑251 (MQ=255)                                                                                                                                                          aaaaCGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCTTGGCGGCAATAACAGAGGCGATGTATGCAACGGGAAGCGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGcc                                                                             <  2:266921/251‑1 (MQ=255)                                                                                                                                                                                ggTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATCGCCGACACGCTGGTCTACATGTTgc                                                       >  2:215874/1‑251 (MQ=255)                                                                                                                                                                                  tagtaTTCAAAACACGCACGTCACTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACAAGCTGGTCTACATGTTgc                                                       >  1:69122/1‑249 (MQ=255)                                                                                                                                                                                                   aCGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGt                                    >  1:178967/1‑251 (MQ=255)                                                                                                                                                                                                      tCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCACTAACAGAGGCGATTTATGCAACGGGGAACGGTCTGGTTAGTGGAAGATGAGCAAGGAATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCgg                                 >  2:160178/1‑250 (MQ=255)                                                                                                                                                                                                                ttAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGGTTTGCCGTCGAGGTCTTTg                         >  2:149482/1‑249 (MQ=255)                                                                                                                                                                                                                     tCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGGCTTTGAGCGAgg                  >  2:81760/1‑251 (MQ=255)                                                                                                                                                                                                                           aaGGTCGTGCGCTATTATGACGCCAAACGCACCGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCCATACCAGAGGCGGTTTATGCAACGGGAAACGGTCTGGATAGTGGAAGATGAGCAAGGGATAGCCGGCACGCTGGTCTACATGTTGCAGCAGGAAGGTTGTTCCGGCGAGGTATTTGAGCGAGGCTTGcc            >  2:157123/2‑251 (MQ=255)                                                                                                                                                                                                                                     gcTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGAt  <  1:10098/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                 CCTTTGATGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACCGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGATGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGAT  >  NC_000913/4635651‑4636129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑