Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,635,757	C→T	A79A (GCC→GCT)	creA →	PF05981 family protein CreA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,635,757	0	C	T	100.0%	40.1 / NA	14	A79A (GCC→GCT)	creA	PF05981 family protein CreA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/4);  total (10/4)

AGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGATGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACCGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGATGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAAC  >  NC_000913/4635531‑4635995                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               aGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGTGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACATCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCg                                                                                                                                                                                                                        >  2:48352/1‑251 (MQ=255)                                gCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGGCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTGGTCAGCAAGTCGGGCCGATTGAACTGTCTGATCGTATTAAAAACGGc                                                                                                                                                                                          >  2:128804/1‑250 (MQ=255)                                                            atttgTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCaaaaa                                                                                                                                                             <  1:7008/247‑1 (MQ=255)                                                                        gACACCGTATTTAAAATGATCGGCCCGTATCACAAAATTGTTGTGGAAGCCTTTGATGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGAGGATTGGGTCTGGCGGAAGATACCTCCGATGAAGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGAGCGTATTAAAAACAGCAAAGCTCAGGGCGAGGTAGTATTCAATAAACGCACGTACCt                                                                                                                                                 >  1:98944/1‑251 (MQ=255)                                                                                aTTTAAAATGATCGGCCCGGATCACCAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGACAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAACAAACGCACGTCCCTGGTCttt                                                                                                                                          >  2:268510/1‑250 (MQ=255)                                                                                                 cGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTg                                                                                                                        >  2:70398/1‑251 (MQ=255)                                                                                                    aTCACACAATTGTTGTGGAAGCCTTTGACGATACCGATGTGAAACATGTCACATGTTATGCGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGCGGCGGCTATTTCTTGTCAGCCAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTAAGGGCGAGGTAGAATTCAAAAAACGCACGTCACTGGTCTTTACGTCGTTACAGGTCGTgcgc                                                                                                                     >  2:258271/1‑251 (MQ=255)                                                                                                                         cctttgtcgatCCCGATGTGAAAAATGTCACCTGTTATGAGAGCCGCGCGCAAACAGGTGGTATTAAAGCAGGGTTGGGTCTAGCGAAAGATACATCCGATGCGGCTATTTCTTGTCAGCAAGTCGAGCCGATTGCACTGTCGGATCGTATTAAAAACGGCAACGCTCAGGGCGAGGAAGTATTCAAAACACGAACGACCCTGGTCTTTAAGTAGTTACAGGACGTGCGCTTTTATGCCGCCAaacgcaa                                                                                                 >  1:111856/9‑250 (MQ=255)                                                                                                                                       caTGTGAAAAATTTCACCTGTTATGTTAGCCGTTCGAAAACAGGTGGTATTAATGGGGGATTGGGTCTGGCGGAAGATTCCTCCGATGCTGCTATTTCGTGTCAGCCAGTCGGTCCGATTGAACTGTCGGATCGTATTAAATACGGCAAAGCTCATGTCGATGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTTCAGTTCGTGCGCTTTTATTACGTCAACCGCAGCGCGCTCGCTTATCt                                                                                  <  2:268456/250‑1 (MQ=255)                                                                                                                                                 aTGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTcc                                                                        >  2:10098/1‑251 (MQ=255)                                                                                                                                                                 gAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAg                                                        <  1:124395/251‑1 (MQ=255)                                                                                                                                                                           aaaaCAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCACGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCgc                                               >  2:13858/1‑249 (MQ=255)                                                                                                                                                                                                       gtcgggCGGAAGATGGCTCCGATGCGGCTATTGCTGGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTGAAGTCGTTACAGGTCGTGCGCTTTGATGTCGGCAGTCGCAACGCGCTCGCTGGTCTGGCTGAGGCCGACAAAGTTGCAGAAGGTACGCCGAAGAACGCGGTGAGCGCGGTGCCTTTCATg                  <  2:17482/247‑1 (MQ=255)                                                                                                                                                                                                                       cTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAAc  >  1:206207/1‑251 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               AGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGATGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACCGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGATGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGAAAAACGCGATTAGCGCGGTTCCTGTCATGCCGTGGCGGCAATAAC  >  NC_000913/4635531‑4635995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑