Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,635,706	C→A	T62T (ACC→ACA)	creA →	PF05981 family protein CreA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,635,706	0	C	A	100.0%	34.3 / NA	14	T62T (ACC→ACA)	creA	PF05981 family protein CreA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/4);  total (10/4)

AATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGATGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACCGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGATGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGA  >  NC_000913/4635487‑4635950                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     aaTTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCgg                                                                                                                                                                                                                       <  1:65131/251‑1 (MQ=255)         gtgtAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGCTCCCGTCCTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGCCACCGTATTTAAAATGATCGGCCCAGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAACCAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATaac                                                                                                                                                                                                               >  1:17482/1‑249 (MQ=255)                                             aGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGTGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACATCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCg                                                                                                                                                                           >  2:48352/1‑251 (MQ=255)                                                                            gCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGGCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTGGTCAGCAAGTCGGGCCGATTGAACTGTCTGATCGTATTAAAAACGGc                                                                                                                                             >  2:128804/1‑250 (MQ=255)                                                                                                        atttgTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCaaaaa                                                                                                                <  1:7008/247‑1 (MQ=255)                                                                                                                    gACACCGTATTTAAAATGATCGGCCCGTATCACAAAATTGTTGTGGAAGCCTTTGATGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGAGGATTGGGTCTGGCGGAAGATACCTCCGATGAAGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGAGCGTATTAAAAACAGCAAAGCTCAGGGCGAGGTAGTATTCAATAAACGCACGTACCt                                                                                                    >  1:98944/1‑251 (MQ=255)                                                                                                                            aTTTAAAATGATCGGCCCGGATCACCAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGACAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAACAAACGCACGTCCCTGGTCttt                                                                                             >  2:268510/1‑250 (MQ=255)                                                                                                                                             cGGATCACAAAATTGTTGTGGAAGCCTTTGACGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTg                                                                           >  2:70398/1‑251 (MQ=255)                                                                                                                                                aTCACACAATTGTTGTGGAAGCCTTTGACGATACCGATGTGAAACATGTCACATGTTATGCGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGCGGCGGCTATTTCTTGTCAGCCAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTAAGGGCGAGGTAGAATTCAAAAAACGCACGTCACTGGTCTTTACGTCGTTACAGGTCGTgcgc                                                                        >  2:258271/1‑251 (MQ=255)                                                                                                                                                                     cctttgtcgatCCCGATGTGAAAAATGTCACCTGTTATGAGAGCCGCGCGCAAACAGGTGGTATTAAAGCAGGGTTGGGTCTAGCGAAAGATACATCCGATGCGGCTATTTCTTGTCAGCAAGTCGAGCCGATTGCACTGTCGGATCGTATTAAAAACGGCAACGCTCAGGGCGAGGAAGTATTCAAAACACGAACGACCCTGGTCTTTAAGTAGTTACAGGACGTGCGCTTTTATGCCGCCAaacgcaa                                                    >  1:111856/9‑250 (MQ=255)                                                                                                                                                                                   caTGTGAAAAATTTCACCTGTTATGTTAGCCGTTCGAAAACAGGTGGTATTAATGGGGGATTGGGTCTGGCGGAAGATTCCTCCGATGCTGCTATTTCGTGTCAGCCAGTCGGTCCGATTGAACTGTCGGATCGTATTAAATACGGCAAAGCTCATGTCGATGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTTCAGTTCGTGCGCTTTTATTACGTCAACCGCAGCGCGCTCGCTTATCt                                     <  2:268456/250‑1 (MQ=255)                                                                                                                                                                                             aTGTCACCTGTTATGTGAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTcc                           >  2:10098/1‑251 (MQ=255)                                                                                                                                                                                                             gAGCCGGGCGAAAACAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAg           <  1:124395/251‑1 (MQ=255)                                                                                                                                                                                                                       aaaaCAGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCTATTTCTTGTCAGCACGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGACGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCgc  >  2:13858/1‑249 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                     AATTGCTCGTGTAATAGATAAAAATGGTAACAATATGAAATACAAGCATTTGATCCTGTCTTTAAGCCTGATAATGCTGGGGCCATTGGCTCATGCAGAAGAGATTGGTTCGGTCGACACCGTATTTAAAATGATCGGCCCGGATCACAAAATTGTTGTGGAAGCCTTTGATGATCCCGATGTGAAAAATGTCACCTGTTATGTGAGCCGGGCGAAAACCGGTGGTATTAAAGGGGGATTGGGTCTGGCGGAAGATACCTCCGATGCGGCCATTTCTTGTCAGCAAGTCGGGCCGATTGAACTGTCGGATCGTATTAAAAACGGCAAAGCTCAGGGCGAGGTAGTATTCAAAAAACGCACGTCCCTGGTCTTTAAGTCGTTACAGGTCGTGCGCTTTTATGATGCCAAACGCAACGCGCTCGCTTATCTGGCTTACTCCGACAAAGTTGTAGAAGGTTCGCCGA  >  NC_000913/4635487‑4635950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑