Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,634,882	C→T	Q143Q (CAG→CAA)	rob ←	DNA‑binding transcriptional dual regulator Rob

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,634,882	0	C	T	100.0%	59.3 / NA	21	Q143Q (CAG→CAA)	rob	DNA‑binding transcriptional dual regulator Rob
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/11);  total (10/11)

TTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAATGGTCGGCGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACC  >  NC_000913/4634642‑4635122                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 ttCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGAGGTGGTATAGAATACCTCTTGCTAGTCGTCTTTATCCTGCCTCAGACGCGTTTCATTCACGCCGTAGCGCACCGGCGGAATGGTCGGCGCGTTGCCGACAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTTTGGGTAacac                                                                                                                                                                                                                                        >  1:159139/1‑251 (MQ=255)      cgccCTGCAGCATCACCGGATGCCCCGTCAGTACCTCGCCATCTGCCTGCTCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAATGGTCGGCGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAAAGATTTGCTCCAGGTAACAGGAGGCGATTTGGGTAACACCaagc                                                                                                                                                                                                                                   >  2:185336/1‑249 (MQ=255)       gccCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAATGGTCGGCGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTTTGGGTAACACCAATCa                                                                                                                                                                                                                                  <  1:196820/251‑1 (MQ=255)                   cACCGGATGCCCCGTCAGTACATAGCCATTTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAATGGTCGGCGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCATCGAACAGGCGTAGCTTTGGGTACCACCAAACAGCAGCGTATCtt                                                                                                                                                                                                                      >  2:3777/1‑251 (MQ=255)                       ggATGCCCCGTCAGTACATCGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAATGGTCGGCGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTTTGGGTAACACCAATCAGCGGCGTATCTTcc                                                                                                                                                                                                                    >  1:211090/1‑249 (MQ=255)                       ggATGACCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAATGGTCGGCGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTTTGGGTAACACCAATCAGCGGCGTATCTTcc                                                                                                                                                                                                                    >  1:213325/1‑249 (MQ=255)                             cccGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAATGGTCGGCGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTTTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGAc                                                                                                                                                                                                            <  1:253391/251‑1 (MQ=255)                                        aTAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAATGGTCGGCGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTTTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGct                                                                                                                                                                                                 <  1:126163/251‑1 (MQ=255)                                                                 aaCGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAATGGTCGGCGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGCTTTGCTCCAGCGCACAAGAGTAGCTTTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGAGACAAATTTGTGCCCTGGCATAGTGAATTCACCCAGGCg                                                                                                                                                                        >  1:25769/1‑251 (MQ=255)                                                                  aCGCGGTTGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAATGGTCGGCGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTTTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGt                                                                                                                                                                       <  2:260356/251‑1 (MQ=255)                                                                   cgcgGTGGTATAGAATACCGCTGGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAATGGTCTGCGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTTTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCTCCCAGGCGTa                                                                                                                                                                      <  1:228770/251‑1 (MQ=255)                                                                            ataGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAATGGTCGGCGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTTTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCggcgggg                                                                                                                                                             >  1:37469/1‑250 (MQ=255)                                                                                         tGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAATGGTCGGCGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTTTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCaaa                                                                                                                                                   <  1:123980/248‑1 (MQ=255)                                                                                         tGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAATGGTCGGCGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTTTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAgg                                                                                                                                                 <  1:29218/250‑1 (MQ=255)                                                                                          gCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAATGGTCGGCGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTTTTGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGAAAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGGGaaaatcaacaggg                                                                                                                                               >  2:197723/1‑240 (MQ=255)                                                                                           cTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAATGGTCGGCGCGTTGCCGAGAAAATCGAGCCAGACCTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCCTTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCGTAGTGAATTCACCCAGGCGTAGCggcgtgcggaatctaaaagcgc                                                                                                                                              >  1:186805/1‑237 (MQ=255)                                                                                                 gtcTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAATGGTCGGCGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTTTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCggcgggggggaacaaaagaggcgcctt                                                                                                                                         >  2:133764/1‑229 (MQ=255)                                                                                                        gccTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAAGGGTCGGCGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGTCGGAAATTAGAGATTTGCTCCAGCGAACAGGAGTAGCTTTGGTTAACACCAATCAGCGGCGAATCTCCCAGTGTGACAAATTTGTTCTCGGTCGTAGTGAATTCACCCAGGCGTATCGGCGGGCGAATACCAAAGGCGCTCCAGTCAGgaga                                                                                                                                 <  2:257457/250‑1 (MQ=255)                                                                                                                                                                                                gCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTTTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGcgcg                                         <  2:211090/251‑1 (MQ=255)                                                                                                                                                                                                       aTGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTTTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGAGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGGTGAGAGTCGAAGCGGGATTGCAGCGCGATGTCCa                                  <  2:37469/251‑1 (MQ=255)                                                                                                                                                                                                                                       aGGAGTAGCTTTGGGGAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGAcc  <  2:134473/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 TTCGCCGCCCTGCAGCATCACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAATGGTCGGCGCGTTGCCGAGAAAATCGTGCCAGAACTGATAACGCATTTCATGGCGGAAATCAGAGATTTGCTCCAGCGAACAGGAGTAGCTCTGGGTAACACCAATCAGCGGCGTATCTTCCAGGGTGACAAATTTGTGCTCTGGCATAGTGAATTCACCCAGGCGTAGCGGCGGGCGAATACCAAAGGCGCTCCATTCAGGAGAACGGCGGTAAAGTGCAGGAGTCTGGGCAAACTGCTTCTTGAATGCGCGGGTAAATGTCTGTTGAGAGTCGAAGCGGTATTGCAGCGCGATGTCCAGAATCGGACGCGCAGTCAGGCGTAGTGCGACC  >  NC_000913/4634642‑4635122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑