Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,630,984 G→T T84T (ACG→ACT)  slt → soluble lytic murein transglycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,630,9840GT92.1% 101.6 / ‑2.9 38T84T (ACG→ACT) sltsoluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (20/15);  total (21/17)
Fisher's exact test for biased strand distribution p-value = 5.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAA‑CCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAA‑AGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTG  >  NC_000913/4630736‑4631229
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       
gAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGGGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGGTGAGCAGCGTAGCCGTTACGAGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGGTGCATCAA‑CCGGCGGTGACgt                                                                                                                                                                                                                                                        >  2:214266/1‑249 (MQ=255)
  aaaaGCCAAACAAGTTACCTGGCGGCTGTTGGTTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAA‑CCGGCGGTGACGGTCa                                                                                                                                                                                                                                                     <  2:92165/251‑1 (MQ=255)
                                   ggCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTtcctc                                                                                                                                                                                                                    <  2:196678/250‑1 (MQ=255)
                                       ggTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCACGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGc                                                                                                                                                                                                                >  1:26910/1‑251 (MQ=255)
                                          gtctgtctGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTTTCCTTAC‑TGGGATACCGGTAGGTCAACGATGGACTGAAGAATCAAACCGCCGGGGATGGCCCCTA‑CTTTTTTTGCGCTAAcccccaagtttccacccctcgc                                                                                                                                                                                                            >  1:164754/1‑233 (MQ=255)
                                              gtctgCTGACGGGCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCAc                                                                                                                                                                                                          <  2:1426/250‑1 (MQ=255)
                                                     gACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGCGCAGCGTAGCCGTTACGCGCAACTCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAAt                                                                                                                                                                                                  >  2:114333/1‑251 (MQ=255)
                                                                     gcgcGAGCCTACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAAATTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACACTGCAATCTCGTTTCGTCAATg                                                                                                                                                                                   >  2:62728/1‑250 (MQ=255)
                                                                          aGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTgg                                                                                                                                                                             >  1:171434/1‑251 (MQ=255)
                                                                                   gcTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTACCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTg                                                                                                                                                                    >  2:47773/2‑251 (MQ=255)
                                                                                      ggATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCTCTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGaa                                                                                                                                                                  <  1:130861/250‑1 (MQ=255)
                                                                                              agcGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGTTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCGCGTTTCGTCAATGAACTGTCGCGGCGTGAAGACTGGCGt                                                                                                                                                         <  2:137019/251‑1 (MQ=255)
                                                                                              agcGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCTCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGTCGCGGCGTGAAGACTGGCGt                                                                                                                                                         <  1:153345/251‑1 (MQ=255)
                                                                                                cGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCACTGAACTGGCGCGGCGTGAAGACTGGCGTgg                                                                                                                                                       >  1:60135/1‑251 (MQ=255)
                                                                                                       gTTACGCGCAAATCAAGCAGGACTGGGCCAATCGAAAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGTTGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTTGTCAATGAACTGGCGCGGCGTGCAGACTGGAGGGGCTTGtt                                                                                                                                                 >  2:62177/1‑250 (MQ=255)
                                                                                                          aCGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGcc                                                                                                                                             >  2:202591/1‑251 (MQ=255)
                                                                                                                aaaTCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGCAGCCTGGACTGAAAGATTCTCCCCTTAACCCCTACCTGGAATACCGTCAGATCACCGATGCCCTGATGAATCAA‑CCGGCGGTAAATGTCACTAACTTTGTTCGCGCTAACACCCCGCTTCCTCCCGCTCGCACGCTGCCATCTCGTTTCGTCAATGAACTGGCGAGGCGTGAAGACTGGCGTGGCTTGTTTGCCTTTAG‑c                                                                                                                                      >  1:243837/1‑251 (MQ=255)
                                                                                                                    cAAGCAGGACTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGCCTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGAATGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACAGGCGTGGCTTGTTTACCTTTAG‑CACgg                                                                                                                                  >  2:223550/1‑251 (MQ=255)
                                                                                                                        cAGGCCTGGGAGAGTCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACGTTATCCCTACCTGGGATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGGCACTAACTTTGTTCGCGCTAACCCCACTCTTGCTCCCGCTTGCACGCTGCAAGCGCGTTTCGTCAAGGAACTGGCGCGGGGTAAAGACTGCCGTGACTGTTTAGCCTTTAT‑CCCTGaaa‑a                                                                                                                             <  2:267198/251‑1 (MQ=255)
                                                                                                                           gCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTTGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAA‑AGcc                                                                                                                          <  2:11688/251‑1 (MQ=255)
                                                                                                                           gCCTGGGATAATCGACAAATGGATGTGGGCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAA‑CCGGCGGTGTCGGTCACTAACTTTGTTCGCGCTAACCTCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTATCCTTTAG‑CCCGGAAG‑AGcc                                                                                                                          <  1:104407/251‑1 (MQ=255)
                                                                                                                                     aTCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTAACCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGCCCTGCTGAATCAA‑CCGGCGGTGACTGACACTAACTTTGTTCGCGATAACCCCCCCCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGCGGCTTGTTAGCCTTTAG‑CCCGGAAA‑Acccccggaatacc                                                                                                                >  1:241951/1‑242 (MQ=255)
                                                                                                                                     aTCGACAAATGGAGGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAA‑AGCCCGGAACTAcc                                                                                                                <  2:45717/251‑1 (MQ=255)
                                                                                                                                                               ctgatgCCTGGAATAAAAGCTTATCCACTTTATCACTAACTGTCATACCGTCAGATCACCGATGACCTGATTAATCAA‑CCGGCGGTGACTGTCACTACCATTGTTAGCGCTAACCCCACGCTTCCTCCCGCTCGCACGTTGCAATCTCGTTTCGTCAATTCACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑TCAGGACA‑AACCCGGAACTACCGAAACGCAATGAAATTCCTACTATgt                                                                                      >  1:213286/2‑250 (MQ=255)
                                                                                                                                                                 gatgCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAA‑AGCCCGGAACTACCGCAGCGCCATGTAATTACTACTATGca                                                                                     >  1:94322/1‑249 (MQ=255)
                                                                                                                                                                                 gATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAA‑AGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGcc                                                                    >  1:131715/1‑250 (MQ=255)
                                                                                                                                                                                       ccacTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAA‑AGCCCGGAACTACCGAAGCGCAATGTAAGTACTAGTATGCAAAATGGATCACCGTGCAGAGTg                                                               <  2:27585/247‑1 (MQ=255)
                                                                                                                                                                                        cccTTTATCCCTACCTGGAATACCGTCAGATTACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAA‑AGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAAGGGAACACCGGGCAGAGTgaa                                                             <  2:26910/249‑1 (MQ=255)
                                                                                                                                                                                                cccTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAA‑AGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCt                                                      <  1:72570/250‑1 (MQ=255)
                                                                                                                                                                                                cccTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAA‑AGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTg                                                     <  2:215121/251‑1 (MQ=255)
                                                                                                                                                                                                   tACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGCCTGTCACTAACTTTGTTCGCGCTAACCCCCCGCTTCCTCCCACTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑GCCGGAAA‑AGCCCGGAACTACCGGAGCCCAAACTAATTACTACTTAGCCAAATGGAAAAACGGGCAAGGGGAAGAAGCCTGGcc                                                  >  1:115941/1‑250 (MQ=255)
                                                                                                                                                                                                      cTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTTGCCTTTAG‑CCCGGAAA‑AGCCCGGAACTAACGAAGCGCAATGTAATTTATACTATGCAAAAAGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAgg                                               >  1:115434/1‑251 (MQ=255)
                                                                                                                                                                                                      cTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAA‑AGCCCGGGACTTCCGAAGCCCCATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGCAGAAGACTgggcagg                                               >  2:96552/1‑246 (MQ=255)
                                                                                                                                                                                                          aaTACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGAGTGATGACTGACGTGGCTTGTTAGGCTTTAGGCCCGGAAA‑‑GCCCCGAACTCCCGAAGCGCAAAGCAATTACCACTCTGCAAAATGGAACACAGGGAAGAGTTAAGAAGCCTGGCCAGGGGCg                                           >  1:23524/1‑251 (MQ=255)
                                                                                                                                                                                                                  cAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAA‑AGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAATGGGCGAAAGAGCt                                   <  2:164754/251‑1 (MQ=255)
                                                                                                                                                                                                                                           aa‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAA‑AGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCt          <  1:202591/251‑1 (MQ=255)
                                                                                                                                                                                                                                                ggcggTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAA‑AGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAAACGGCAAGAGCCAGCCTAACg      >  1:193259/1‑251 (MQ=255)
                                                                                                                                                                                                                                                ggcggTGACTGTCACTAACTTTGTTCGCAATAACCCCACGCTTCCTCCCGCTCGCAAGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAACAACCCG‑ACCTACCGAAGCGCAATGTAATTACTCCTATGCAACATGGAACACCGGGCACAGTGAAGAAGACTGGCAAGGGGGGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACg      >  2:12473/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    gTGACTGTCACTAACGTTGTTCGCGCTAACCCCACGCTTCCTCTCGCTCGCACGCTGCAATCTCGTTTCGTCAATGACCTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTTGCCTTTAG‑CCCGGAAA‑AGCCCGGATCTACCGAAGCGCAATGTAATGCCTACTATGCAAAATGTAACACCGGGCAGAGGGAAGAAGCCTGGCAAGGGGCGAAAGCGCTGTGGCTAACCGGCAAGAGACAGTCTAACGCCtg  <  2:207464/251‑1 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       
GAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAA‑CCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAA‑AGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCTGTGGCTAACCGGCAAGAGCCAGCCTAACGCCTG  >  NC_000913/4630736‑4631229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑