Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,630,960	T→C	D76D (GAT→GAC)	slt →	soluble lytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,630,960	0	T	C	94.4%	102.1 / ‑4.1	37	D76D (GAT→GAC)	slt	soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (20/14);  total (21/16)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

GAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAA‑CCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCT  >  NC_000913/4630721‑4631196                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               gagGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCa                                                                                                                                                                                                                                     <  2:51203/251‑1 (MQ=255)         gcTTGTGGACAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGTGCAGCGTAGCCGTTACGCGCAAATCAAGAAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑Ccggcgg                                                                                                                                                                                                                            >  2:181235/1‑251 (MQ=255)                gAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGGGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGGTGAGCAGCGTAGCCGTTACGAGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGGTGCATCAA‑CCGGCGGTGACgt                                                                                                                                                                                                                      >  2:214266/1‑249 (MQ=255)                  aaaaGCCAAACAAGTTACCTGGCGGCTGTTGGTTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAA‑CCGGCGGTGACGGTCa                                                                                                                                                                                                                   <  2:92165/251‑1 (MQ=255)                                                   ggCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTtcctc                                                                                                                                                                                  <  2:196678/250‑1 (MQ=255)                                                       ggTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCACGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGc                                                                                                                                                                              >  1:26910/1‑251 (MQ=255)                                                          gtctgtctGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTTTCCTTAC‑TGGGATACCGGTAGGTCAACGATGGACTGAAGAATCAAACCGCCGGGGATGGCCCCTA‑CTTTTTTTGCGCTAAcccccaagtttccacccctcgc                                                                                                                                                                          >  1:164754/1‑233 (MQ=255)                                                              gtctgCTGACGGGCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCAc                                                                                                                                                                        <  2:1426/250‑1 (MQ=255)                                                                     gACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGCGCAGCGTAGCCGTTACGCGCAACTCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAAt                                                                                                                                                                >  2:114333/1‑251 (MQ=255)                                                                                     gcgcGAGCCTACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAAATTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACACTGCAATCTCGTTTCGTCAATg                                                                                                                                                 >  2:62728/1‑250 (MQ=255)                                                                                          aGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTgg                                                                                                                                           >  1:171434/1‑251 (MQ=255)                                                                                                   gcTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTACCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTg                                                                                                                                  >  2:47773/2‑251 (MQ=255)                                                                                                      ggATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCTCTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGaa                                                                                                                                <  1:130861/250‑1 (MQ=255)                                                                                                              agcGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGTTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCGCGTTTCGTCAATGAACTGTCGCGGCGTGAAGACTGGCGt                                                                                                                       <  2:137019/251‑1 (MQ=255)                                                                                                              agcGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCTCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGTCGCGGCGTGAAGACTGGCGt                                                                                                                       <  1:153345/251‑1 (MQ=255)                                                                                                                cGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCACTGAACTGGCGCGGCGTGAAGACTGGCGTgg                                                                                                                     >  1:60135/1‑251 (MQ=255)                                                                                                                       gTTACGCGCAAATCAAGCAGGACTGGGCCAATCGAAAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGTTGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTTGTCAATGAACTGGCGCGGCGTGCAGACTGGAGGGGCTTGtt                                                                                                               >  2:62177/1‑250 (MQ=255)                                                                                                                          aCGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGcc                                                                                                           >  2:202591/1‑251 (MQ=255)                                                                                                                                aaaTCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGCAGCCTGGACTGAAAGATTCTCCCCTTAACCCCTACCTGGAATACCGTCAGATCACCGATGCCCTGATGAATCAA‑CCGGCGGTAAATGTCACTAACTTTGTTCGCGCTAACACCCCGCTTCCTCCCGCTCGCACGCTGCCATCTCGTTTCGTCAATGAACTGGCGAGGCGTGAAGACTGGCGTGGCTTGTTTGCCTTTAG‑c                                                                                                    >  1:243837/1‑251 (MQ=255)                                                                                                                                    cAAGCAGGACTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGCCTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGAATGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACAGGCGTGGCTTGTTTACCTTTAG‑CACgg                                                                                                >  2:223550/1‑251 (MQ=255)                                                                                                                                        cAGGCCTGGGAGAGTCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACGTTATCCCTACCTGGGATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGGCACTAACTTTGTTCGCGCTAACCCCACTCTTGCTCCCGCTTGCACGCTGCAAGCGCGTTTCGTCAAGGAACTGGCGCGGGGTAAAGACTGCCGTGACTGTTTAGCCTTTAT‑CCCTGaaaa                                                                                            <  2:267198/251‑1 (MQ=255)                                                                                                                                           gCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTTGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAAAGcc                                                                                         <  2:11688/251‑1 (MQ=255)                                                                                                                                           gCCTGGGATAATCGACAAATGGATGTGGGCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAA‑CCGGCGGTGTCGGTCACTAACTTTGTTCGCGCTAACCTCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTATCCTTTAG‑CCCGGAAGAGcc                                                                                         <  1:104407/251‑1 (MQ=255)                                                                                                                                                     aTCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTAACCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGCCCTGCTGAATCAA‑CCGGCGGTGACTGACACTAACTTTGTTCGCGATAACCCCCCCCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGCGGCTTGTTAGCCTTTAG‑CCCGGAAAAcccccggaatacc                                                                               >  1:241951/1‑242 (MQ=255)                                                                                                                                                     aTCGACAAATGGAGGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAAAGCCCGGAACTAcc                                                                               <  2:45717/251‑1 (MQ=255)                                                                                                                                                                               ctgatgCCTGGAATAAAAGCTTATCCACTTTATCACTAACTGTCATACCGTCAGATCACCGATGACCTGATTAATCAA‑CCGGCGGTGACTGTCACTACCATTGTTAGCGCTAACCCCACGCTTCCTCCCGCTCGCACGTTGCAATCTCGTTTCGTCAATTCACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑TCAGGACAAACCCGGAACTACCGAAACGCAATGAAATTCCTACTATgt                                                     >  1:213286/2‑250 (MQ=255)                                                                                                                                                                                 gatgCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAAAGCCCGGAACTACCGCAGCGCCATGTAATTACTACTATGca                                                    >  1:94322/1‑249 (MQ=255)                                                                                                                                                                                                 gATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGcc                                   >  1:131715/1‑250 (MQ=255)                                                                                                                                                                                                       ccacTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAAGTACTAGTATGCAAAATGGATCACCGTGCAGAGTg                              <  2:27585/247‑1 (MQ=255)                                                                                                                                                                                                        cccTTTATCCCTACCTGGAATACCGTCAGATTACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAAGGGAACACCGGGCAGAGTgaa                            <  2:26910/249‑1 (MQ=255)                                                                                                                                                                                                                cccTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCt                     <  1:72570/250‑1 (MQ=255)                                                                                                                                                                                                                cccTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTg                    <  2:215121/251‑1 (MQ=255)                                                                                                                                                                                                                   tACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGCCTGTCACTAACTTTGTTCGCGCTAACCCCCCGCTTCCTCCCACTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑GCCGGAAAAGCCCGGAACTACCGGAGCCCAAACTAATTACTACTTAGCCAAATGGAAAAACGGGCAAGGGGAAGAAGCCTGGcc                 >  1:115941/1‑250 (MQ=255)                                                                                                                                                                                                                      cTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTTGCCTTTAG‑CCCGGAAAAGCCCGGAACTAACGAAGCGCAATGTAATTTATACTATGCAAAAAGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAgg              >  1:115434/1‑251 (MQ=255)                                                                                                                                                                                                                      cTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAAAGCCCGGGACTTCCGAAGCCCCATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGCAGAAGACTgggcagg              >  2:96552/1‑246 (MQ=255)                                                                                                                                                                                                                          aaTACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGAGTGATGACTGACGTGGCTTGTTAGGCTTTAGGCCCGGAAA‑GCCCCGAACTCCCGAAGCGCAAAGCAATTACCACTCTGCAAAATGGAACACAGGGAAGAGTTAAGAAGCCTGGCCAGGGGCg          >  1:23524/1‑251 (MQ=255)                                                                                                                                                                                                                                  cAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAATGGGCGAAAGAGCt  <  2:164754/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               GAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAA‑CCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAG‑CCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCAGAGTGAAGAAGCCTGGCAAGGGGCGAAAGAGCT  >  NC_000913/4630721‑4631196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑