Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,630,921	G→A	P63P (CCG→CCA)	slt →	soluble lytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,630,921	0	G	A	93.1%	70.3 / ‑1.4	31	P63P (CCG→CCA)	slt	soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (17/10);  total (18/13)
Fisher's exact test for biased strand distribution p-value = 1.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-01

ATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAA‑CCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCA  >  NC_000913/4630678‑4631163                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    aTCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATc                                                                                                                                                                                                                                              >  1:137019/1‑251 (MQ=255)        aCGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCt                                                                                                                                                                                                                                       <  1:217107/251‑1 (MQ=255)              cATGACGCTGCATTAATGTATTTACACTTAGAGGATGCGCTGGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTTGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATa                                                                                                                                                                                                                                 <  2:151125/251‑1 (MQ=255)                                            gagGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCa                                                                                                                                                                                                   <  2:51203/251‑1 (MQ=255)                                                    gcTTGTGGACAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGTGCAGCGTAGCCGTTACGCGCAAATCAAGAAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑Ccggcgg                                                                                                                                                                                          >  2:181235/1‑251 (MQ=255)                                                           gAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGGGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGGTGAGCAGCGTAGCCGTTACGAGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGGTGCATCAA‑CCGGCGGTGACgt                                                                                                                                                                                    >  2:214266/1‑249 (MQ=255)                                                             aaaaGCCAAACAAGTTACCTGGCGGCTGTTGGTTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAA‑CCGGCGGTGACGGTCa                                                                                                                                                                                 <  2:92165/251‑1 (MQ=255)                                                                                              ggCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTtcctc                                                                                                                                                <  2:196678/250‑1 (MQ=255)                                                                                                  ggTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCACGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGc                                                                                                                                            >  1:26910/1‑251 (MQ=255)                                                                                                     gtctgtctGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTTTCCTTAC‑TGGGATACCGGTAGGTCAACGATGGACTGAAGAATCAAACCGCCGGGGATGGCCCCTA‑CTTTTTTTGCGCTAAcccccaagtttccacccctcgc                                                                                                                                        >  1:164754/1‑233 (MQ=255)                                                                                                         gtctgCTGACGGGCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCAc                                                                                                                                      <  2:1426/250‑1 (MQ=255)                                                                                                                gACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGCGCAGCGTAGCCGTTACGCGCAACTCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAAt                                                                                                                              >  2:114333/1‑251 (MQ=255)                                                                                                                                gcgcGAGCCTACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAAATTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACACTGCAATCTCGTTTCGTCAATg                                                                                                               >  2:62728/1‑250 (MQ=255)                                                                                                                                     aGCCGACTCACTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTgg                                                                                                         >  1:171434/1‑251 (MQ=255)                                                                                                                                              gcTGGATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTACCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTg                                                                                                >  2:47773/2‑251 (MQ=255)                                                                                                                                                 ggATGAGCAGCGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCTCTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGaa                                                                                              <  1:130861/250‑1 (MQ=255)                                                                                                                                                         agcGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGTTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCGCGTTTCGTCAATGAACTGTCGCGGCGTGAAGACTGGCGt                                                                                     <  2:137019/251‑1 (MQ=255)                                                                                                                                                         agcGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCTCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGTCGCGGCGTGAAGACTGGCGt                                                                                     <  1:153345/251‑1 (MQ=255)                                                                                                                                                           cGTAGCCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCACTGAACTGGCGCGGCGTGAAGACTGGCGTgg                                                                                   >  1:60135/1‑251 (MQ=255)                                                                                                                                                                  gTTACGCGCAAATCAAGCAGGACTGGGCCAATCGAAAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGTTGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTTGTCAATGAACTGGCGCGGCGTGCAGACTGGAGGGGCTTGtt                                                                             >  2:62177/1‑250 (MQ=255)                                                                                                                                                                     aCGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGcc                                                                         >  2:202591/1‑251 (MQ=255)                                                                                                                                                                           aaaTCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGCAGCCTGGACTGAAAGATTCTCCCCTTAACCCCTACCTGGAATACCGTCAGATCACCGATGCCCTGATGAATCAA‑CCGGCGGTAAATGTCACTAACTTTGTTCGCGCTAACACCCCGCTTCCTCCCGCTCGCACGCTGCCATCTCGTTTCGTCAATGAACTGGCGAGGCGTGAAGACTGGCGTGGCTTGTTTGCCTTTAGc                                                                   >  1:243837/1‑251 (MQ=255)                                                                                                                                                                               cAAGCAGGACTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGCCTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGAATGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACAGGCGTGGCTTGTTTACCTTTAGCACgg                                                               >  2:223550/1‑251 (MQ=255)                                                                                                                                                                                   cAGGCCTGGGAGAGTCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACGTTATCCCTACCTGGGATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGGCACTAACTTTGTTCGCGCTAACCCCACTCTTGCTCCCGCTTGCACGCTGCAAGCGCGTTTCGTCAAGGAACTGGCGCGGGGTAAAGACTGCCGTGACTGTTTAGCCTTTATCCCTGaaaa                                                           <  2:267198/251‑1 (MQ=255)                                                                                                                                                                                      gCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTTGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGcc                                                        <  2:11688/251‑1 (MQ=255)                                                                                                                                                                                      gCCTGGGATAATCGACAAATGGATGTGGGCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAA‑CCGGCGGTGTCGGTCACTAACTTTGTTCGCGCTAACCTCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTATCCTTTAGCCCGGAAGAGcc                                                        <  1:104407/251‑1 (MQ=255)                                                                                                                                                                                                aTCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAAGATTAACCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGCCCTGCTGAATCAA‑CCGGCGGTGACTGACACTAACTTTGTTCGCGATAACCCCCCCCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGCGGCTTGTTAGCCTTTAGCCCGGAAAAcccccggaatacc                                              >  1:241951/1‑242 (MQ=255)                                                                                                                                                                                                aTCGACAAATGGAGGTGGTCGAACAAATGATGCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTAcc                                              <  2:45717/251‑1 (MQ=255)                                                                                                                                                                                                                          ctgatgCCTGGAATAAAAGCTTATCCACTTTATCACTAACTGTCATACCGTCAGATCACCGATGACCTGATTAATCAA‑CCGGCGGTGACTGTCACTACCATTGTTAGCGCTAACCCCACGCTTCCTCCCGCTCGCACGTTGCAATCTCGTTTCGTCAATTCACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGTCAGGACAAACCCGGAACTACCGAAACGCAATGAAATTCCTACTATgt                    >  1:213286/2‑250 (MQ=255)                                                                                                                                                                                                                            gatgCCTGGACTGAAAGATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGCAGCGCCATGTAATTACTACTATGca                   >  1:94322/1‑249 (MQ=255)                                                                                                                                                                                                                                            gATTATCCACTTTATCCCTACCTGGAATACCGTCAGATCACCGATGACCTGATGAATCAA‑CCGGCGGTGACTGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCAAAATGGAACACCGGGcc  >  1:131715/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    ATCTATTACGCGGCATGACGCTGCATTGATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCTGGAATACCGCCAGATCACCGATGATCTGATGAATCAA‑CCGGCGGTGACGGTCACTAACTTTGTTCGCGCTAACCCCACGCTTCCTCCCGCTCGCACGCTGCAATCTCGTTTCGTCAATGAACTGGCGCGGCGTGAAGACTGGCGTGGCTTGTTAGCCTTTAGCCCGGAAAAGCCCGGAACTACCGAAGCGCAATGTAATTACTACTATGCGAAATGGAACACCGGGCA  >  NC_000913/4630678‑4631163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑