Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,630,400 A→G N41N (AAT→AAC)  ettA ← energy‑dependent translational throttle protein EttA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,630,4000AG96.2% 65.2 / ‑4.2 26N41N (AAT→AAC) ettAenergy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (13/12);  total (14/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01

CAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTA  >  NC_000913/4630153‑4630643
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   
cAGCGGCCAGCTTGTCAAAATCGGCACCCGGATCGGCGTACAGCGCATCCACTTCATCCAGGCGTTTCAGGGCGTTACCCACTTCAGAACCCGCTTCTTAAATGGACTCCCGCACGGTGTGTCCCGGTTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCCCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTc                                                                                                                                                                                                                                                  <  1:157241/251‑1 (MQ=255)
     gCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGAcc                                                                                                                                                                                                                                             >  1:170305/1‑251 (MQ=255)
       aagcttttaaaaaTCGGAATCCGGATGGGGGTAAAGCGCATACACTTCATCAGGGCTTTTAAGGGCGTTAACCTCTTAAAAACCCGATTCTTAAATGGACTAACGAACGGTGGGTTCCGGGTTCAGCGGCGGTTCCGGCGGCAGTAAACCAATTTGGATGGCGGGCTGCGGACGCGCTCCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCa                                                                                                                                                                                                                                           <  1:118493/242‑1 (MQ=255)
        aGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGAccc                                                                                                                                                                                                                                            >  1:151125/1‑249 (MQ=255)
        aGCTTGTAAAAATCGGCACCCGGACCGGCGTACAGCGAATACCCTTCACCCGGGGTTTTCGGGGCGTTACCCCCTTCAGAACCCGCTTCTTCAAGGGACTCCCGCAGGGTGTGTTCCGGGTTCAGCGGCGGTTCCTGCGGCAGGTACCCATTCTTGATGTCGGGCGGCGGACGCGCTTCCCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAg                                                                                                                                                                                                                                          <  2:70498/251‑1 (MQ=255)
              aaaaaaTCGGCTCCCGGATCGGCGTACAGCGCATACCCTTCACCCAGGCTTTTCAGGGCGTTACCCCCTTAAGAACCCTCTTCTTCAATGGACCCCCGCAGGGTGTGTCCCGGGTTCGGCGGCGGTTCCTGCGGCAGGAAACCAATCTGGATGTCGGGCGGCGGACGCGCTTCCCTTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACAcc                                                                                                                                                                                                                                    <  1:26288/249‑1 (MQ=255)
                               aTCGGCGTACAGCGCATACACTTCATCAAGGCGTTTCAGGGAGTTAACCACTTCAGAAACCCCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGGAGCGCTTCCCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTTGACTTACCCGCGCCGTTCAGACCCAGGACACCCATTTTTGCCCCAggg                                                                                                                                                                                                                    >  2:181360/1‑250 (MQ=255)
                                     gTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAAc                                                                                                                                                                                                             >  1:82361/1‑251 (MQ=255)
                                                  ccTTCATCCAGGCGTTCCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATTGCCTCACGCACGGTGTGTCCCGGGTTCAGCGGCGTTTCCGGCGGCAGGTAACCAATCTTGTTGTCTGCCTGCGGACGCCTTTCACCTCCGATGTCTTTACAAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGtt                                                                                                                                                                                                <  1:51042/250‑1 (MQ=255)
                                                       aTCCAGGCGTTTCAGGGCGTTAACCACTTCAAAACCCCCTTCTTCAAGGGACTCCCGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAACCTTGATGTCTGGCTGCGGACGCTCTTCCCCTCCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCa                                                                                                                                                                                           <  1:181360/251‑1 (MQ=255)
                                                                                                         aCTCACGCACGGGGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCGTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGACGATCAGACCCAGGACACCAATTTTTGACCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACCACTTTGCCGACACGATGCATGGtata                                                                                                                                          >  2:72333/1‑250 (MQ=255)
                                                                                                                   ggTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGGGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAAATCAGAGAGATGTTTTTCAAAATATGACGTTTAGGCGGAACAACTTTTCCGACACGATGCATGGTATAAACGaaatc                                                                                                                                 >  1:232917/1‑246 (MQ=255)
                                                                                                                              ggTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGCCGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCCGAGAGATGGTTTTCAAAATATGACGTTTCGGCGGAAAAACTTTGCCGCCACGATGCCTGGTATAAAAGACTTGAGCCACggttga                                                                                                                     >  2:217107/1‑245 (MQ=255)
                                                                                                                              ggTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGCTGCATGGTATAAACGAATTGAGCCAc                                                                                                                           >  1:269243/1‑244 (MQ=255)
                                                                                                                                      tGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCctc                                                                                                            >  1:73619/1‑251 (MQ=255)
                                                                                                                                         ggTTCCTGGGGCGGTTACCCAATCTTGTTGTCTGGCTGCGGACGCGTTTCACCTTCGATGTTTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTAt                                                                                                         <  1:49412/251‑1 (MQ=255)
                                                                                                                                                             aaTCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGtt                                                                                     >  2:235056/1‑251 (MQ=255)
                                                                                                                                                               tCTTGATGTCTGGCTGCGGACGCGCTTCAACTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGtttt                                                                                   >  1:92165/1‑251 (MQ=255)
                                                                                                                                                                                  aCGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGAAACCAATTTTTGCCCCAGGGACGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCAAGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCt                                                                >  1:267198/1‑251 (MQ=255)
                                                                                                                                                                                                 gttgtttttACCAAGGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAAt                                                 <  1:231348/245‑1 (MQ=255)
                                                                                                                                                                                                                 cccGCCATAATGCGCAGAAGGGTTGACTGACCCGCGCCGTTCAGACACAGGACACCAATTTTTGCCCCAGGGAAGAAACTTAGATAGATGTTTTTCAAAATTTGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTAGAAACGAATTGAGCCACGTTGGACTTCGCCTTTATGTTTATCGGGATTAGGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCATTTGGTCGATCa                                 <  2:245877/251‑1 (MQ=255)
                                                                                                                                                                                                                            gcgcAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCTAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCagt                       <  1:171732/250‑1 (MQ=255)
                                                                                                                                                                                                                                   agGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGCAGTGTAGCCTTTT‑CCCCGCCTAATCCCAGTTGGTCGTTCACCTTCGCagtaaaagtaa               >  1:32281/1‑250 (MQ=255)
                                                                                                                                                                                                                                      gtagACTTACCCGCGCCGTTCAGACCCAGGTCTCCAATTTTTGCCCCAGGTAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCTACACGATGCATGGTATAAACGAATTTAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTaaaaa            <  1:213717/248‑1 (MQ=255)
                                                                                                                                                                                                                                             tACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAAAAAGTGTc      <  1:20169/250‑1 (MQ=255)
                                                                                                                                                                                                                                                 cgcgCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGACACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGCACAACTTTGCCGACACGATGCCTGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATGATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGTTGGTCGATCACCTTCGCAGTAAAAGTAAACAAGTGTCCGTa  >  1:29461/1‑250 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   
CAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCAAAATATGACGTTTCGGCGGAACAACTTTGCCGACACGATGCATGGTATAAACGAATTGAGCCACGTTGGACTTCGCCTCTATGTTTATCGTGATAATGAGTTTTCAAAGGCGAAGTGTAGCCTTTTTCCCCGCCTAATCCCAGCCGGTCGATCACCCTCGCAGTAAAAGTAAAAAAGTGTCCGTA  >  NC_000913/4630153‑4630643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑