Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,630,238 T→A S95S (TCA→TCT)  ettA ← energy‑dependent translational throttle protein EttA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,630,2380TA96.7% 90.7 / NA 30S95S (TCA→TCT) ettAenergy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (8/21);  minor base C (1/0);  total (9/21)
Fisher's exact test for biased strand distribution p-value = 3.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCA  >  NC_000913/4629991‑4630458
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                            
gcagcagGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCATTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTGATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGaa                                                                                                                                                                                                                           <  1:136378/251‑1 (MQ=255)
   gcagGCGGCACAACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAAcc                                                                                                                                                                                                                        <  2:5766/251‑1 (MQ=255)
            acaACGCTACGCGACGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCCGAAACCGCttcttc                                                                                                                                                                                                                >  2:56877/1‑250 (MQ=255)
                     cgcgAAGACGCTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTca                                                                                                                                                                                                      <  2:48648/251‑1 (MQ=255)
                            acgctCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACgg                                                                                                                                                                                               <  1:170100/247‑1 (MQ=255)
                                    caccGGAGAGGTTAGCGATTTTAGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGCTCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGAGTACCGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTcc                                                                                                                                                                                       >  2:231348/1‑251 (MQ=255)
                                       cGGAGTGGTTACCGATTTTCGCGTCCCGGTCCGGCAGACGTCGCTCTTCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGCCCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTGGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCACACACTTCATCCAGGCGTTTCAGGGCGTGAACCACTTCAGAAACCGCTTCTTCAATGGACGCACGCACGGTGTGTTCCggg                                                                                                                                                                                    <  2:197133/251‑1 (MQ=255)
                                                tAGCGATTTTCGCGCCCCAGTCCGGCAGACGTAGCGCCTCCGCCGCACGCTCCAGCTTTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAAGCGGCATCCGGATCGGCGTACAGCGCATGCACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTg                                                                                                                                                                            <  2:91300/250‑1 (MQ=255)
                                                             gTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGc                                                                                                                                                               >  1:70498/1‑250 (MQ=255)
                                                                ccgggccggccgtcGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCGCTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAggt                                                                                                                                                           <  2:258189/238‑3 (MQ=255)
                                                                ccAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAggt                                                                                                                                                           <  1:61012/251‑3 (MQ=255)
                                                                                           cgcACGCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGAc                                                                                                                                <  1:75930/251‑1 (MQ=255)
                                                                                                gCTCCAGCTGTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTACTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCt                                                                                                                           >  1:193749/1‑251 (MQ=255)
                                                                                                         gTACGTTCAGGTTATGTCCGTCGTGAGCCCGAATGATCTCTTCCAGCCGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTCCAGCTCATACACTTCAGCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACTGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCtt                                                                                                                    <  2:260321/249‑1 (MQ=255)
                                                                                                         gTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCtt                                                                                                                    <  1:238509/249‑1 (MQ=255)
                                                                                                         gTACGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCAGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTc                                                                                                                   <  1:188428/250‑1 (MQ=255)
                                                                                                           aCGTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGAt                                                                                                                <  1:252366/251‑1 (MQ=255)
                                                                                                             gTTCAGGTTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGt                                                                                                              <  1:37440/251‑1 (MQ=255)
                                                                                                                                                    cagccGGCCTTGTTCAGCGGCCAGCTTGGCAAAATCGGCATCCGTATCGGCGTACAGCGCATACCCTTCATCCAGGCGTTTCAGGGCGTTAACCCCTTCAGAACCCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAAGGCCCGCCATAATGCGCAGCAGGGTAGACt                                                                       <  1:56877/247‑1 (MQ=255)
                                                                                                                                                           ccTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCcgc                                                                >  2:19305/1‑251 (MQ=255)
                                                                                                                                                                  cAGCGGCCAGCTTGTCAAAATCGGCACCCGGATCGGCGTACAGCGCATCCACTTCATCCAGGCGTTTCAGGGCGTTACCCACTTCAGAACCCGCTTCTTAAATGGACTCCCGCACGGTGTGTCCCGGTTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCCCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTc                                                         <  1:157241/251‑1 (MQ=255)
                                                                                                                                                                       gCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGAcc                                                    >  1:170305/1‑251 (MQ=255)
                                                                                                                                                                         aagcttttaaaaaTCGGAATCCGGATGGGGGTAAAGCGCATACACTTCATCAGGGCTTTTAAGGGCGTTAACCTCTTAAAAACCCGATTCTTAAATGGACTAACGAACGGTGGGTTCCGGGTTCAGCGGCGGTTCCGGCGGCAGTAAACCAATTTGGATGGCGGGCTGCGGACGCGCTCCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCa                                                  <  1:118493/242‑1 (MQ=255)
                                                                                                                                                                          aGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGAccc                                                   >  1:151125/1‑249 (MQ=255)
                                                                                                                                                                          aGCTTGTAAAAATCGGCACCCGGACCGGCGTACAGCGAATACCCTTCACCCGGGGTTTTCGGGGCGTTACCCCCTTCAGAACCCGCTTCTTCAAGGGACTCCCGCAGGGTGTGTTCCGGGTTCAGCGGCGGTTCCTGCGGCAGGTACCCATTCTTGATGTCGGGCGGCGGACGCGCTTCCCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAg                                                 <  2:70498/251‑1 (MQ=255)
                                                                                                                                                                                aaaaaaTCGGCTCCCGGATCGGCGTACAGCGCATACCCTTCACCCAGGCTTTTCAGGGCGTTACCCCCTTAAGAACCCTCTTCTTCAATGGACCCCCGCAGGGTGTGTCCCGGGTTCGGCGGCGGTTCCTGCGGCAGGAAACCAATCTGGATGTCGGGCGGCGGACGCGCTTCCCTTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACAcc                                           <  1:26288/249‑1 (MQ=255)
                                                                                                                                                                                                 aTCGGCGTACAGCGCATACACTTCATCAAGGCGTTTCAGGGAGTTAACCACTTCAGAAACCCCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGGAGCGCTTCCCCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTTGACTTACCCGCGCCGTTCAGACCCAGGACACCCATTTTTGCCCCAggg                           >  2:181360/1‑250 (MQ=255)
                                                                                                                                                                                                       gTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAAc                    >  1:82361/1‑251 (MQ=255)
                                                                                                                                                                                                                    ccTTCATCCAGGCGTTCCAGGGCGTTAACCACTTCAGAAACCGCTTCTTCAATTGCCTCACGCACGGTGTGTCCCGGGTTCAGCGGCGTTTCCGGCGGCAGGTAACCAATCTTGTTGTCTGCCTGCGGACGCCTTTCACCTCCGATGTCTTTACAAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGtt       <  1:51042/250‑1 (MQ=255)
                                                                                                                                                                                                                         aTCCAGGCGTTTCAGGGCGTTAACCACTTCAAAACCCCCTTCTTCAAGGGACTCCCGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGGTAACCAACCTTGATGTCTGGCTGCGGACGCTCTTCCCCTCCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTAGACTTACCCGCGCCGTTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCa  <  1:181360/251‑1 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                            
GCAGCAGGCGGCACAACGCTACGCGACGACGTTCACCACCGGAGAGGTTAGCGATTTTCGCGTCCCAGTCCGGCAGACGTAGCGCATCCGCCGCACGCTCCAGCTGTACGTTCAGATTATGACCGTCGTGAGCCTGAATGATCTCTTCCAGACGGCCTTGTTCAGCGGCCAGCTTGTCAAAATCGGCATCCGGATCGGCGTACAGCGCATACACTTCATCCAGGCGTTTCAGGGCGTTAACCACTTCTGAAACCGCTTCTTCAATGGACTCACGCACGGTGTGTTCCGGGTTCAGCTGCGGTTCCTGCGGCAGATAACCAATCTTGATGTCTGGCTGCGGACGCGCTTCACCTTCGATGTCTTTATCAATGCCCGCCATAATGCGCAGCAGGGTGGACTTACCCGCGCCATTCAGACCCAGGACACCAATTTTTGCCCCAGGGAAGAAACTCAGAGAGATGTTTTTCA  >  NC_000913/4629991‑4630458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑