| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,629,635 | T→C | E296E (GAA→GAG) | ettA ← | energy‑dependent translational throttle protein EttA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,629,635 | 0 | T | C | 97.1% | 90.8 / NA | 35 | E296E (GAA→GAG) | ettA | energy‑dependent translational throttle protein EttA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (25/9); minor base A (1/0); total (26/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
CGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGG > NC_000913/4629398‑4629875 | cGGCTGTTCCTGACCAGAGATCATACGGAACAGGGGCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCATTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGCTATTCGGTGATGTTCAGCTCTTCaaaacgtg > 2:200102/1‑246 (MQ=255) gTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGcc > 1:2936/1‑248 (MQ=255) tCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGAc < 1:54303/250‑1 (MQ=255) gACCAGAGATCAAACGGAACAGGTTCGATTGCCCCGCGCCGTGCGGACCGATGATCCCGACGATTGCCCCTGTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGAGTTACGCAGGTTGCTGTCTTCCAGCACTTTATCGCCCAGACGCGGTTCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGGTGCGGAGATTCGGTGCGGTTCAGCTCTTCAAAGCGTGCCAGACGTGCt < 2:22306/251‑1 (MQ=255) ccAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCGTGCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTGCTGATATTCGGTGCGGTTCAGCTCTTCAAAGCGTGCCAGACGTGCttt < 1:220176/251‑1 (MQ=255) aTCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGGCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTCCGTTTCTGATCTTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCttt > 1:165275/1‑251 (MQ=255) cGATGTACCCGCGCAGGTCGGACCGATGAACCCTACTATTGTCCCTTCCGGGATCGAGAAGCTCAGGTCATCAATCAGCGGTCGATCACCGTAGTATGTACGCAGGTTACTGACTGCCAGCACTTTATCGCCCAGACGCGGGCCAGGGGGAATAAACAGTTCGTTGGCTTCTTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACTTGCTTTACCTTTCGACTGGCGGCCTTTAg < 2:107160/251‑1 (MQ=255) tttACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGCCTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGGTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGttc > 1:197615/1‑249 (MQ=255) ttACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGCTATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGttcc > 2:241194/1‑248 (MQ=255) ggACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCCGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTAACCactc > 2:158239/1‑248 (MQ=255) cgacgaTTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTACAGCACTTTATCGCCCAGACGCGGTCCAGATGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGAGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTtctcg > 1:28930/1‑250 (MQ=255) tttCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTacgac > 1:132892/1‑251 (MQ=255) aaGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGAAGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTCCGACGCGCAGCTTCTtg > 1:122173/1‑251 (MQ=255) aGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTtgt > 1:91300/1‑251 (MQ=255) gCTCAGGTCATCAATCAGCAGGCGATCACCGGAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCACAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTAGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTtgtg > 1:78619/1‑251 (MQ=255) cTCAGGACATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGACCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCAAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGAAGTACCCAATCCAGCTCTTTCTCGCTCGACTTACGACGCGCCGCTTCTtgtg > 1:119254/1‑250 (MQ=255) atcaatcaGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCt > 2:140105/1‑251 (MQ=255) tcaatcaGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTg > 1:155804/1‑251 (MQ=255) atcaGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTgcgc > 1:78664/1‑251 (MQ=255) tcaGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGcc > 2:102583/1‑251 (MQ=255) caggcGATCACCGTAGGATTTACGAAGGTTGCTGACGTCCAGCATGTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTGCGTTACGTTTCTGATATTCGGTGCTGTGCAGCTCTTCAAAGCGTGCCATACGTGCCTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCATCTCGTTCTCGATCGACTCACGACGCGCCGCTTCTTGTGAATCTTCCTGCGCCAGgc < 2:102195/247‑1 (MQ=255) tGCTGACTTCCAGCACTTTATCGCACAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCaggagggg > 2:145044/1‑249 (MQ=255) cTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGttg > 1:103532/1‑250 (MQ=255) tCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGttgcc > 1:48910/1‑248 (MQ=255) ccAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCt > 1:231651/1‑251 (MQ=255) cACTTTATCGACCAGAAGCGGGCCAGGTGGCATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGCTCAGCTCTTCAAAGCATGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGGGGAGCAGTTGCCTTccc > 2:258537/1‑251 (MQ=255) ttATCGCCGAGACGAGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGTTGTTGAGATCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCGTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCTGGAGGAGTAGTTGCCTTCCCACgg > 2:119954/1‑251 (MQ=255) ggTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACcgcg > 1:98768/1‑251 (MQ=255) cAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGTCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGACTTCCCACGGAATACCTTCACCGCGGTc > 1:7628/1‑250 (MQ=255) gTTGGTTTCGTTACGTTTCTGATATTCGTTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTGTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCGCCAGCCAGGAGGAGTAGTTTCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTg < 1:225382/251‑1 (MQ=255) ttCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGt > 1:90704/1‑251 (MQ=255) gATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCGTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATTCCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGt < 1:159828/249‑1 (MQ=255) tCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTTCCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGt < 1:200102/250‑1 (MQ=255) gttcTGTTCCGCTCTTCAAAGCGTGCCCGCAGTGCTTTACCTTTCGACGGGCGGACTTTAGTTCCCTGACGTCCCCACTCCAGCTCGTTCTCGATCGACTTACGACGCGCCGCTCCTTGTGAAGCTGCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTACCCACGGAATACCTTCACCGCGGTCAAGTGCGAGGATCCAGCCTGCAACGGTAGCGAGGAAGTTACGGTCGt < 2:165275/247‑1 (MQ=255) tgattttCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCCGCTCTTTCTCGATCGACTTACGACGCGCCGCTTCTTGTGACGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTGCCTTCCCACGGACTCCCTTCACCGCGGTCAAGTTCGAGGATCCAGCCAGCAACGTTATCGAGGAGGTAACGGTCGTgg > 2:238509/6‑251 (MQ=255) | CGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCAATCGACTTACGACGCGCCGCTTCTTGTGAAGCTTCCTGCGCCAGGCGCTGATCTTTCTGCTCCAGCCAGGAGGAGTAGTTACCTTCCCACGGAATACCTTCACCGCGGTCAAGTTCGAGGATCCAGCCTGCAACGTTATCGAGGAAGTAACGGTCGTGG > NC_000913/4629398‑4629875 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |