Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,629,476	T→C	G349G (GGA→GGG)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,629,476	0	T	C	100.0%	92.3 / NA	32	G349G (GGA→GGG)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/13);  total (19/13)

TAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATGATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGACGCCAGTTTCACCGTTTCACCCAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCA  >  NC_000913/4629243‑4629716                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 gaGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCGAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGaa                                                                                                                                                                                                                                 <  1:25334/250‑1 (MQ=255)                      gtgtTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCCCGGAACTGATCAACCGCTGCCAGTATCCCCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCgtcaatcaac                                                                                                                                                                                                              >  1:181548/1‑247 (MQ=255)                               aTCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATcacc                                                                                                                                                                                                   >  1:109890/1‑248 (MQ=255)                                              aGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGTTCACCGTAGGATTTACGc                                                                                                                                                                                      >  1:49623/1‑249 (MQ=255)                                                      ggAAACTTCTTCCCAAACGGCTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACAGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGCGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGCAGATCAGGTCATCAATCAGCCGGCGATCACCGTAGGATTTACGCAGGTTGCTgc                                                                                                                                                                            >  1:22306/1‑250 (MQ=255)                                                          aCTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGAATTc                                                                                                                                                                        >  2:52964/1‑251 (MQ=255)                                                                             tGCTGTTATCCAGTGAGGCACGGAACTGATCAACCGATGCCAGTTTCCCCGTTTCACCTAAAGTGATGGGGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTGCCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAg                                                                                                                                                      <  2:32620/250‑1 (MQ=255)                                                                               cTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCTCTTTCGGGATCGAGAAGCTCAGGGCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCCGCACTTTATCGCCCAGAcg                                                                                                                                                   >  2:199109/1‑251 (MQ=255)                                                                                     tCCATTGATGCACGGAACTGATCAACCGATGCCAGGTTCACCGGTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGGACAGAGATCATACGGAACAGTGTCGAGTTTCCCGCTCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAAGCAGCAGGCGATCACCGTAGGGTTTACGCAGGTTGCTGACTTCCAGCACTTGATCGCCCAGActtggtc                                                                                                                                              <  2:237210/250‑7 (MQ=255)                                                                                                               cgatgcccgtttcaccgtttcaccTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCCGGTGGACTAAACAGTTCGTTGGttt                                                                                                                   >  2:265995/9‑251 (MQ=255)                                                                                                                 atgCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGCTCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACTGTTCGTTGGTTTCg                                                                                                                 >  1:227399/3‑251 (MQ=255)                                                                                                                      aGTTTCACCTGTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACg                                                                                                            <  1:104920/251‑1 (MQ=255)                                                                                                                           cgccgtttccccTAAAGTGATGGTGCCGCTGTCCGGCTGGTCCTGGCCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGTCGATGGCCCCTTTCGGGATCGAGAAGCTCGGGTCAGCAATCAGCCGGCTATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGGCGCGGTCCAGGGGGAATAAACAGTTCGTTGGTGTCGTTACTTTTc                                                                                                        <  2:126274/248‑1 (MQ=255)                                                                                                                           caccgtttcaccTAAAGCGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCt                                                                                                       <  1:116467/251‑1 (MQ=255)                                                                                                                               gtttcaacTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGata                                                                                                   <  2:109890/251‑1 (MQ=255)                                                                                                                                        aacgTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACACGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGAAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCt                                                                                          >  2:159625/4‑251 (MQ=255)                                                                                                                                              aTGGTGCCGCTGTCCGGCTGTTCCTGACCAGAAATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTc                                                                                      >  1:200082/1‑249 (MQ=255)                                                                                                                                                            cGGCTGTTCCTGACCAGAGATCATACGGAACAGGGGCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCATTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGCTATTCGGTGATGTTCAGCTCTTCaaaacgtg                                                                      >  2:200102/1‑246 (MQ=255)                                                                                                                                                                 gTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGcc                                                                    >  1:2936/1‑248 (MQ=255)                                                                                                                                                                   tCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGAc                                                                <  1:54303/250‑1 (MQ=255)                                                                                                                                                                       gACCAGAGATCAAACGGAACAGGTTCGATTGCCCCGCGCCGTGCGGACCGATGATCCCGACGATTGCCCCTGTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGAGTTACGCAGGTTGCTGTCTTCCAGCACTTTATCGCCCAGACGCGGTTCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGGTGCGGAGATTCGGTGCGGTTCAGCTCTTCAAAGCGTGCCAGACGTGCt                                                           <  2:22306/251‑1 (MQ=255)                                                                                                                                                                         ccAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCGTGCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTGCTGATATTCGGTGCGGTTCAGCTCTTCAAAGCGTGCCAGACGTGCttt                                                         <  1:220176/251‑1 (MQ=255)                                                                                                                                                                              gaTCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCCGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTc                                                                                                                  >  2:47072/1‑189 (MQ=255)                                                                                                                                                                              gaTCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTc                                                                                                                  <  1:47072/189‑1 (MQ=255)                                                                                                                                                                               aTCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGGCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTCCGTTTCTGATCTTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCttt                                                   >  1:165275/1‑251 (MQ=255)                                                                                                                                                                                                cGATGTACCCGCGCAGGTCGGACCGATGAACCCTACTATTGTCCCTTCCGGGATCGAGAAGCTCAGGTCATCAATCAGCGGTCGATCACCGTAGTATGTACGCAGGTTACTGACTGCCAGCACTTTATCGCCCAGACGCGGGCCAGGGGGAATAAACAGTTCGTTGGCTTCTTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACTTGCTTTACCTTTCGACTGGCGGCCTTTAg                                  <  2:107160/251‑1 (MQ=255)                                                                                                                                                                                                   tttACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGCCTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGGTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGttc                               >  1:197615/1‑249 (MQ=255)                                                                                                                                                                                                    ttACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGCTATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGttcc                              >  2:241194/1‑248 (MQ=255)                                                                                                                                                                                                                 tCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGTCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCTGTCCAAGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGtgc                                                                                      <  2:175962/182‑3 (MQ=255)                                                                                                                                                                                                                 tCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTc                                                                                      >  1:175962/1‑182 (MQ=255)                                                                                                                                                                                                                   ggACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCCGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTAACCactc               >  2:158239/1‑248 (MQ=255)                                                                                                                                                                                                                                cgacgaTTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTACAGCACTTTATCGCCCAGACGCGGTCCAGATGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGAGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGTTCCCTGACGTACCCACTCCAGCTCTTtctcg  >  1:28930/1‑250 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                 TAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATGATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGACGCCAGTTTCACCGTTTCACCCAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACCTTGACGTACCCATTCCAGCTCTTTCTCA  >  NC_000913/4629243‑4629716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑