| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,629,446 | A→G | G359G (GGT→GGC) | ettA ← | energy‑dependent translational throttle protein EttA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,629,446 | 0 | A | G | 96.9% | 86.2 / NA | 32 | G359G (GGT→GGC) | ettA | energy‑dependent translational throttle protein EttA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (19/12); minor base T (0/1); total (19/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
AACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATGATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGACGCCAGTTTCACCGTTTCACCCAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACC > NC_000913/4629215‑4629688 | aaCCCCTTTAAAGTAAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGACGATTTACCCGCGCCGTTCGGACCGATGATcc > 2:54303/1‑251 (MQ=255) aaagttaaagCGGCCAACGTAGGCGCGGCTTTGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGCGATCCTACGGAACAGGGTCGATTTACCCGCGCCGTTCGGCCCGATGATCCCTACGAttg > 2:220176/1‑249 (MQ=255) aaagttaaagCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGAttg < 1:837/251‑3 (MQ=255) aagCGGACAACGTAGGCGCGGCTTGGCATCGCGGTGTTGCCGATCTTCATAATATCTAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGACACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTCAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCCGAGATCATACGGAACCGGGTCGGTTTACCCGCGCCGTTCGGACCGCTGATCCagacgcttgccccttt > 1:51462/1‑240 (MQ=255) gaGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCGAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGaa < 1:25334/250‑1 (MQ=255) gtgtTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCCCGGAACTGATCAACCGCTGCCAGTATCCCCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCgtcaatcaac > 1:181548/1‑247 (MQ=255) aTCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATcacc > 1:109890/1‑248 (MQ=255) aGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGTTCACCGTAGGATTTACGc > 1:49623/1‑249 (MQ=255) ggAAACTTCTTCCCAAACGGCTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACAGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGCGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGCAGATCAGGTCATCAATCAGCCGGCGATCACCGTAGGATTTACGCAGGTTGCTgc > 1:22306/1‑250 (MQ=255) aCTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGAATTc > 2:52964/1‑251 (MQ=255) tGCTGTTATCCAGTGAGGCACGGAACTGATCAACCGATGCCAGTTTCCCCGTTTCACCTAAAGTGATGGGGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTGCCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAg < 2:32620/250‑1 (MQ=255) cTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCTCTTTCGGGATCGAGAAGCTCAGGGCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCCGCACTTTATCGCCCAGAcg > 2:199109/1‑251 (MQ=255) tCCATTGATGCACGGAACTGATCAACCGATGCCAGGTTCACCGGTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGGACAGAGATCATACGGAACAGTGTCGAGTTTCCCGCTCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAAGCAGCAGGCGATCACCGTAGGGTTTACGCAGGTTGCTGACTTCCAGCACTTGATCGCCCAGActtggtc < 2:237210/250‑7 (MQ=255) cgatgcccgtttcaccgtttcaccTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCCGGTGGACTAAACAGTTCGTTGGttt > 2:265995/9‑251 (MQ=255) atgCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGCTCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACTGTTCGTTGGTTTCg > 1:227399/3‑251 (MQ=255) aGTTTCACCTGTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACg < 1:104920/251‑1 (MQ=255) cgccgtttccccTAAAGTGATGGTGCCGCTGTCCGGCTGGTCCTGGCCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGTCGATGGCCCCTTTCGGGATCGAGAAGCTCGGGTCAGCAATCAGCCGGCTATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGGCGCGGTCCAGGGGGAATAAACAGTTCGTTGGTGTCGTTACTTTTc < 2:126274/248‑1 (MQ=255) caccgtttcaccTAAAGCGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCt < 1:116467/251‑1 (MQ=255) gtttcaacTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGata < 2:109890/251‑1 (MQ=255) aacgTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACACGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGAAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCt > 2:159625/4‑251 (MQ=255) aTGGTGCCGCTGTCCGGCTGTTCCTGACCAGAAATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTc > 1:200082/1‑249 (MQ=255) cGGCTGTTCCTGACCAGAGATCATACGGAACAGGGGCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCATTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGCTATTCGGTGATGTTCAGCTCTTCaaaacgtg > 2:200102/1‑246 (MQ=255) gTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGcc > 1:2936/1‑248 (MQ=255) tCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGAc < 1:54303/250‑1 (MQ=255) gACCAGAGATCAAACGGAACAGGTTCGATTGCCCCGCGCCGTGCGGACCGATGATCCCGACGATTGCCCCTGTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGAGTTACGCAGGTTGCTGTCTTCCAGCACTTTATCGCCCAGACGCGGTTCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGGTGCGGAGATTCGGTGCGGTTCAGCTCTTCAAAGCGTGCCAGACGTGCt < 2:22306/251‑1 (MQ=255) ccAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCGTGCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTGCTGATATTCGGTGCGGTTCAGCTCTTCAAAGCGTGCCAGACGTGCttt < 1:220176/251‑1 (MQ=255) gaTCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCCGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTc > 2:47072/1‑189 (MQ=255) gaTCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTc < 1:47072/189‑1 (MQ=255) aTCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGGCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTCCGTTTCTGATCTTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCttt > 1:165275/1‑251 (MQ=255) cGATGTACCCGCGCAGGTCGGACCGATGAACCCTACTATTGTCCCTTCCGGGATCGAGAAGCTCAGGTCATCAATCAGCGGTCGATCACCGTAGTATGTACGCAGGTTACTGACTGCCAGCACTTTATCGCCCAGACGCGGGCCAGGGGGAATAAACAGTTCGTTGGCTTCTTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACTTGCTTTACCTTTCGACTGGCGGCCTTTAg < 2:107160/251‑1 (MQ=255) tttACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGCCTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGGTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGttc > 1:197615/1‑249 (MQ=255) ttACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGCTATTCGGTGCTGTTCAGCTCTTCAAAGCGTGCCAGACGTGCTTTACCTTTCGACTGGCGGCCTTTAGttcc > 2:241194/1‑248 (MQ=255) | AACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATGATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGACGCCAGTTTCACCGTTTCACCCAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTCCAGGTGGAATAAACAGTTCGTTGGTTTCGTTACGTTTCTGATATTCGGTGCTGTTCAGTTCTTCAAAGCGCGCCAGACGTGCTTTACCTTTCGACTGACGGCCTTTAGTACC > NC_000913/4629215‑4629688 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |