Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,629,356	C→T	A389A (GCG→GCA)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,629,356	0	C	T	100.0%	58.6 / NA	20	A389A (GCG→GCA)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/7);  total (13/7)

AGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAGAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATGATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGACGCCAGTTTCACCGTTTCACCCAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTC  >  NC_000913/4629120‑4629576                                                                                                                                                                                                                                             |                                                                                                                                                                                                                             ggcagcagcaTGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACGGATGCCAgtttcaccgt                                                                                                                                                                                                                <  2:88967/250‑1 (MQ=255)                               agcTTCGCCAGATGCAGACGACAGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCgg                                                                                                                                                                                  >  1:102195/1‑251 (MQ=255)                                       cAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCt                                                                                                                                                                          <  1:134577/251‑1 (MQ=255)                                             cAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGAGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTTTTCCTGACCag                                                                                                                                                                    <  2:226636/251‑1 (MQ=255)                                                 cgacCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCag                                                                                                                                                                    >  1:126274/1‑247 (MQ=255)                                                         cTCACCACCGGACAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGAGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACgg                                                                                                                                                         >  2:25334/1‑250 (MQ=255)                                                                  ggaaagTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAACGCGGCAAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCAGCCGGAAACTTTTTCCCAACCGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCGGTTTCACCGTTTCACCTAACGTGTTGGTGCCGCTGCCCGGCTGTTCCTGCCCAGAGAACATCCGCAACAggggcg                                                                                                                                               >  2:225382/5‑248 (MQ=255)                                                                                                aaCCCCTTTAAAGTAAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGACGATTTACCCGCGCCGTTCGGACCGATGATcc                                                                                                                 >  2:54303/1‑251 (MQ=255)                                                                                                         aaagttaaagCGGCCAACGTAGGCGCGGCTTTGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGCGATCCTACGGAACAGGGTCGATTTACCCGCGCCGTTCGGCCCGATGATCCCTACGAttg                                                                                                        >  2:220176/1‑249 (MQ=255)                                                                                                         aaagttaaagCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGAttg                                                                                                        <  1:837/251‑3 (MQ=255)                                                                                                                aagCGGACAACGTAGGCGCGGCTTGGCATCGCGGTGTTGCCGATCTTCATAATATCTAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGACACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTCAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCCGAGATCATACGGAACCGGGTCGGTTTACCCGCGCCGTTCGGACCGCTGATCCagacgcttgccccttt                                                                                                 >  1:51462/1‑240 (MQ=255)                                                                                                                            gaGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCGAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGaa                                                                                     <  1:25334/250‑1 (MQ=255)                                                                                                                                                 gtgtTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCCCGGAACTGATCAACCGCTGCCAGTATCCCCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCgtcaatcaac                                                                  >  1:181548/1‑247 (MQ=255)                                                                                                                                                          aTCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATcacc                                                       >  1:109890/1‑248 (MQ=255)                                                                                                                                                                         aGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGTTCACCGTAGGATTTACGc                                          >  1:49623/1‑249 (MQ=255)                                                                                                                                                                                 ggAAACTTCTTCCCAAACGGCTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACAGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGCGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGCAGATCAGGTCATCAATCAGCCGGCGATCACCGTAGGATTTACGCAGGTTGCTgc                                >  1:22306/1‑250 (MQ=255)                                                                                                                                                                                     aCTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGAATTc                            >  2:52964/1‑251 (MQ=255)                                                                                                                                                                                                        tGCTGTTATCCAGTGAGGCACGGAACTGATCAACCGATGCCAGTTTCCCCGTTTCACCTAAAGTGATGGGGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTGCCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAg          <  2:32620/250‑1 (MQ=255)                                                                                                                                                                                                          cTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCTCTTTCGGGATCGAGAAGCTCAGGGCATCAATCAGCAGGCGATCACCGTAGGATTTACGCAGGTTGCTGACTTCCCGCACTTTATCGCCCAGAcg       >  2:199109/1‑251 (MQ=255)                                                                                                                                                                                                                tCCATTGATGCACGGAACTGATCAACCGATGCCAGGTTCACCGGTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGGACAGAGATCATACGGAACAGTGTCGAGTTTCCCGCTCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAAGCAGCAGGCGATCACCGTAGGGTTTACGCAGGTTGCTGACTTCCAGCACTTGATCGCCCAGActtggtc  <  2:237210/250‑7 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                             AGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAGAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATGATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGACGCCAGTTTCACCGTTTCACCCAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCCATAGGATTTACGCAGGTTGCTGACTTCCAGCACTTTATCGCCCAGACGCGGTC  >  NC_000913/4629120‑4629576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑