Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,629,281	G→A	I414I (ATC→ATT)	ettA ←	energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,629,281	0	G	A	100.0%	72.1 / NA	23	I414I (ATC→ATT)	ettA	energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/10);  total (13/10)

GAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAGAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATGATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGACGCCAGTTTCACCGTTTCACCCAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCC  >  NC_000913/4629053‑4629523                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   gAACTCCAGCAGGGCGTTGTCCAGCGCGCGCAGGGTTCCGATATCCAGGTCGTGGGTCGGTTCGTCGGGCAGCAGCAGGTTGCCGCCAACCTGCAGCCGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCAACAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAActt                                                                                                                                                                                                                              <  2:204194/251‑1 (MQ=255)                  tttCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCGGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGtttt                                                                                                                                                                                                              <  1:17923/250‑1 (MQ=255)                      cAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGt                                                                                                                                                                                                         >  1:107160/1‑251 (MQ=255)                                 gggTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAg                                                                                                                                                                                              <  1:236502/251‑1 (MQ=255)                                     ttcgacacccAGCTCGTTGGTTGGTTCGTAGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGACCGTTCCCCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCCCGGCTTGGCCTCTCGGTGTTGACGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCCCACGGTTTTTCCGTTATCCATTGAGCCAc                                                                                                                                                                                          >  2:159828/9‑251 (MQ=255)                                          tatCCAGGTAGTTGGATGGTTCGTCGAGCAGCAGCATGTTGACGCAAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGAATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAACCGCTTTTGCTGTTATCCATTGAGTCACGGAAc                                                                                                                                                                                     >  2:45726/1‑251 (MQ=255)                                              cAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAACCGGTTTTGCTGTTATCCATTGAGTCACTGAACTGAt                                                                                                                                                                                 <  1:56550/251‑1 (MQ=255)                                                                    ggcagcagcaTGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACGGATGCCAgtttcaccgt                                                                                                                                                           <  2:88967/250‑1 (MQ=255)                                                                                                  agcTTCGCCAGATGCAGACGACAGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCgg                                                                                                                             >  1:102195/1‑251 (MQ=255)                                                                                                          cAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCt                                                                                                                     <  1:134577/251‑1 (MQ=255)                                                                                                                cAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGAGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTTTTCCTGACCag                                                                                                               <  2:226636/251‑1 (MQ=255)                                                                                                                    cgacCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCag                                                                                                               >  1:126274/1‑247 (MQ=255)                                                                                                                     gacCGCGCTCACCACCGGAACGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACg                                                                                                                                                                                                                   >  1:231404/1‑146 (MQ=255)                                                                                                                     gacCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCTGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGTCGGAAACGTCTTCCCAAACg                                                                                                                                                                                                                   <  2:231404/146‑1 (MQ=255)                                                                                                                            cTCACCACCGGACAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGAGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACgg                                                                                                    >  2:25334/1‑250 (MQ=255)                                                                                                                                     ggaaagTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAACGCGGCAAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCAGCCGGAAACTTTTTCCCAACCGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCGGTTTCACCGTTTCACCTAACGTGTTGGTGCCGCTGCCCGGCTGTTCCTGCCCAGAGAACATCCGCAACAggggcg                                                                                          >  2:225382/5‑248 (MQ=255)                                                                                                                                                                   aaCCCCTTTAAAGTAAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGACGATTTACCCGCGCCGTTCGGACCGATGATcc                                                            >  2:54303/1‑251 (MQ=255)                                                                                                                                                                            aaagttaaagCGGCCAACGTAGGCGCGGCTTTGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGCGATCCTACGGAACAGGGTCGATTTACCCGCGCCGTTCGGCCCGATGATCCCTACGAttg                                                   >  2:220176/1‑249 (MQ=255)                                                                                                                                                                            aaagttaaagCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGAttg                                                   <  1:837/251‑3 (MQ=255)                                                                                                                                                                                   aagCGGACAACGTAGGCGCGGCTTGGCATCGCGGTGTTGCCGATCTTCATAATATCTAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGACACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTCAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCCGAGATCATACGGAACCGGGTCGGTTTACCCGCGCCGTTCGGACCGCTGATCCagacgcttgccccttt                                            >  1:51462/1‑240 (MQ=255)                                                                                                                                                                                               gaGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCGAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGaa                                <  1:25334/250‑1 (MQ=255)                                                                                                                                                                                                                    gtgtTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCCCGGAACTGATCAACCGCTGCCAGTATCCCCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCgtcaatcaac             >  1:181548/1‑247 (MQ=255)                                                                                                                                                                                                                             aTCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCGCCGTTCGGACCGATGATCCCGACGATTGCCCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGGCGATcacc  >  1:109890/1‑248 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   GAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAGAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATGATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGACGCCAGTTTCACCGTTTCACCCAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGGAACAGGGTCGATTTACCCGCACCGTTCGGACCGATGATCCCGACGATCGCTCCTTTCGGGATCGAGAAGCTCAGGTCATCAATCAGCAGACGATCGCC  >  NC_000913/4629053‑4629523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑