Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,629,188 G→A L445L (CTC→CTT)  ettA ← energy‑dependent translational throttle protein EttA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,629,1880GA93.5% 90.4 / NA 31L445L (CTC→CTT) ettAenergy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (10/19);  minor base C (2/0);  total (12/19)
Fisher's exact test for biased strand distribution p-value = 1.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

AGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAGAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATGATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGACGCCAGTTTCACCGTTTCACCCAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGG  >  NC_000913/4628944‑4629425
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             
aGTTACCTTCGAAGAACTCAACTTTACCTTCCTCCTGGTAGTCCAGGATGTGCGTGGCGATAAGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAAATCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTc                                                                                                                                                                                                                                          >  2:104920/1‑250 (MQ=255)
    aCCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCaa                                                                                                                                                                                                                                     <  1:77185/251‑1 (MQ=255)
     ccTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAAc                                                                                                                                                                                                                                    <  2:47753/251‑1 (MQ=255)
     ccTTCGAAGAACTCAACTTTACCTTCATCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAAc                                                                                                                                                                                                                                    <  2:4745/251‑1 (MQ=255)
                               aTCCTGGTAGTCCAGGATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTttaa                                                                                                                                                                                                          <  1:82391/251‑1 (MQ=255)
                                       agtCCAGGATGTGCGTGGCGATACGGGCGAGGAACCAACGGGCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTGCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCTCCAACCTGCAGCAGCTTCGCCAGATGCAGACTACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCTCTttaaagttaaa                                                                                                                                                                                                   <  2:163896/248‑1 (MQ=255)
                                           caggaTGTGCGTGGCGATACGGTCGAGGACCCAACGGGCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGc                                                                                                                                                                                              <  2:213514/247‑1 (MQ=255)
                                              gaTGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTGCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCATCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCaa                                                                                                                                                                                           <  2:153412/250‑1 (MQ=255)
                                                       ggCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACATCCCGGGAACTCTAGCAGGGCGTCTTCCAGCGCGCGCAGGGTTTCGATATCCATGTCGTTGGTTGTTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACTCTTTTACCCTGATCAATCCCTTTAAAGTTAAAGCGGCCAACGTAGgctc                                                                                                                                                                                  <  1:218328/251‑3 (MQ=255)
                                                       ggCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGgcgc                                                                                                                                                                                  <  1:218325/251‑1 (MQ=255)
                                                                         ccAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGACCTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGgtgg                                                                                                                                                                >  2:116467/1‑250 (MQ=255)
                                                                             cGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTa                                                                                                                                                                                       <  2:213410/224‑1 (MQ=255)
                                                                             cGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTa                                                                                                                                                                                       >  1:213410/1‑224 (MQ=255)
                                                                              ggTCGTGCGAGATAACCATCGCACCGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCg                                                                                                                                                           >  2:236502/1‑251 (MQ=255)
                                                                                                      aGCCCGGGTACTCCAGAAGGGCGTTTTCCAGCGCGCGAAGGGTTTAGATATCCAGGTAGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCAAGATGCAGACGACCGCGCTCACCACCGGAAAGt                                                                                                                                                                                                                                            >  2:254633/1‑146 (MQ=255)
                                                                                                      aGCCCGGGAACTCCAGCAGTGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGt                                                                                                                                                                                                                                            <  1:254633/146‑1 (MQ=255)
                                                                                                             gAACTCCAGCAGGGCGTTGTCCAGCGCGCGCAGGGTTCCGATATCCAGGTCGTGGGTCGGTTCGTCGGGCAGCAGCAGGTTGCCGCCAACCTGCAGCCGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCAACAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAActt                                                                                                                            <  2:204194/251‑1 (MQ=255)
                                                                                                                              tttCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCGGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGtttt                                                                                                            <  1:17923/250‑1 (MQ=255)
                                                                                                                                  cAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGt                                                                                                       >  1:107160/1‑251 (MQ=255)
                                                                                                                                             gggTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAg                                                                                            <  1:236502/251‑1 (MQ=255)
                                                                                                                                                 ttcgacacccAGCTCGTTGGTTGGTTCGTAGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGACCGTTCCCCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCCCGGCTTGGCCTCTCGGTGTTGACGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCCCACGGTTTTTCCGTTATCCATTGAGCCAc                                                                                        >  2:159828/9‑251 (MQ=255)
                                                                                                                                                      tatCCAGGTAGTTGGATGGTTCGTCGAGCAGCAGCATGTTGACGCAAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGAATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAACCGCTTTTGCTGTTATCCATTGAGTCACGGAAc                                                                                   >  2:45726/1‑251 (MQ=255)
                                                                                                                                                          cAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAACCGGTTTTGCTGTTATCCATTGAGTCACTGAACTGAt                                                                               <  1:56550/251‑1 (MQ=255)
                                                                                                                                                                                ggcagcagcaTGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACGGATGCCAgtttcaccgt                                                         <  2:88967/250‑1 (MQ=255)
                                                                                                                                                                                                              agcTTCGCCAGATGCAGACGACAGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCgg                           >  1:102195/1‑251 (MQ=255)
                                                                                                                                                                                                                      cAGATGCAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCt                   <  1:134577/251‑1 (MQ=255)
                                                                                                                                                                                                                            cAGACGACCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGAGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTTTTCCTGACCag             <  2:226636/251‑1 (MQ=255)
                                                                                                                                                                                                                                cgacCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCag             >  1:126274/1‑247 (MQ=255)
                                                                                                                                                                                                                                 gacCGCGCTCACCACCGGAACGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACg                                                                                                                 >  1:231404/1‑146 (MQ=255)
                                                                                                                                                                                                                                 gacCGCGCTCACCACCGGAAAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCTGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGTCGGAAACGTCTTCCCAAACg                                                                                                                 <  2:231404/146‑1 (MQ=255)
                                                                                                                                                                                                                                        cTCACCACCGGACAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGAGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATAATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGATGCCAGTTTCACCGTTTCACCTAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACgg  >  2:25334/1‑250 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             
AGTTACCTTCGAAGAACTCAACTTTACCTTCATCCTGGTAATCCAGAATGTGCGTGGCGATACGGTCGAGGAACCAACGGTCGTGCGAGATAACCATCGCACAGCCCGGGAACTCCAGCAGGGCGTTTTCCAGCGCGCGCAGGGTTTCGATATCCAGGTCGTTGGTTGGTTCGTCGAGCAGCAGCATGTTGCCGCCAACCTGCAGCAGCTTCGCCAGATGCAGACGACCGCGCTCACCACCGGAGAGTTCACCAACGCGTTTACCCTGATCAACCCCTTTAAAGTTAAAGCGGCCAACGTAGGCGCGGCTTGGCATCTCGGTGTTGCCGATCTTCATGATATCCAGCCCGCCGGAAACTTCTTCCCAAACGGTTTTGCTGTTATCCATTGAGTCACGGAACTGATCAACCGACGCCAGTTTCACCGTTTCACCCAAAGTGATGGTGCCGCTGTCCGGCTGTTCCTGACCAGAGATCATACGG  >  NC_000913/4628944‑4629425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑