Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,628,721	C→T	intergenic (+174/+134)	nadR → / ← ettA	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,628,721	0	C	T	100.0%	102.2 / NA	32	intergenic (+174/+134)	nadR/ettA	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/11);  total (21/11)

ATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGC‑TGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACT  >  NC_000913/4628483‑4628966                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       aTTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCgg                                                                                                                                                                                                                                           >  1:67974/1‑251 (MQ=255) aTTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACATCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGCGATCCGCTACGAAATTGCGGCTACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCgg                                                                                                                                                                                                                                           >  2:76106/1‑251 (MQ=255)  ttatgACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGt                                                                                                                                                                                                                                          >  1:236371/5‑251 (MQ=255)      gACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGGGATGAAACGGCTTAAAGGCGAGGTAAAAAATAAGTTTTTG‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCTTTCACGCCGTATCCGGCATTCGGTGCt                                                                                                                                                                                                                                       <  2:101854/250‑1 (MQ=255)                              gagcTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCACAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGCTGCGACGCTTACcgc                                                                                                                                                                                                              >  2:98574/4‑251 (MQ=255)                                        gggAGATGCTGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATACAATAAGTTTTTT‑GATGAGTTGCAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCttctc                                                                                                                                                                                                      >  2:161325/1‑246 (MQ=255)                                                               tAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCCTCTTATCAGGCCtggagattcacaccgtattg                                                                                                                                                                             >  2:77185/1‑232 (MQ=255)                                                                           aacggCTCAACGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGCTTCACAACGTATTGAAAtttcttggt                                                                                                                                                                 >  2:52066/5‑244 (MQ=255)                                                                                cTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAAtttcttggtttgt                                                                                                                                                             >  2:69036/1‑239 (MQ=255)                                                                                  cAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAgg                                                                                                                                                         >  1:204194/1‑251 (MQ=255)                                                                                          aGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATa                                                                                                                                                  >  2:119861/1‑250 (MQ=255)                                                                                                   aataagttttttGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTc                                                                                                                                          >  1:61836/7‑250 (MQ=255)                                                                                                         ttttttGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCAcgccgc                                                                                                                                   >  1:25981/1‑251 (MQ=255)                                                                                                                   aGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACACCGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAtt                                                                                                                         >  2:72598/1‑251 (MQ=255)                                                                                                                    gTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTGTTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAttt                                                                                                                        >  1:46514/1‑251 (MQ=255)                                                                                                                    gTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAGGGCGTTCACGCCGCATCCGGCAttt                                                                                                                        >  1:200834/1‑251 (MQ=255)                                                                                                                                   ggaggagGCTGACGAGATAAACCAAGGTTTGAAAAAACCTCCACGGGTGCCCCGCTACGAAATTGCGGATACTAAGGCGTTTGTAGGCCGGAAAAGGCGTTCCCGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAGGGCGTTCACGCCGCATCCGGCATTTTACGCATTACtt                                                                                                            <  2:200834/244‑1 (MQ=255)                                                                                                                                         ggcgtcagagaATAAACAAGGTTTGAAAAACCCTGCACGGGGGACCCGCTACGAAATTGCGGAAACTAAGTCTGTGGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACg                                                                                                   <  2:121243/244‑1 (MQ=255)                                                                                                                                           cTGTCGCGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGTCGCATCCGGCATTTTACGCATTACTTCGCAATACGCt                                                                                                 >  1:213514/1‑251 (MQ=255)                                                                                                                                               cGAGATTAAACAAGGTTTAAAAAACCCTGCACGGGTGACCCGCTCCGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGt                                                                                              <  1:72598/250‑1 (MQ=255)                                                                                                                                                        aCAAGGTTTGAAAAAACCTACAGGGGTGACCCCCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACg                                                                                    <  2:46514/251‑1 (MQ=255)                                                                                                                                                                          tGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTc                                                                  >  2:73289/1‑251 (MQ=255)                                                                                                                                                                           gCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCCGTGCGTCt                                                                 >  2:106752/1‑251 (MQ=255)                                                                                                                                                                             aCGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAACGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATACGGCCTTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCCGTGCGTCTgc                                                               >  2:95064/1‑251 (MQ=255)                                                                                                                                                                                           ccaaaaTTGCGGATGTTAGGGCTGTGGAAGGCCGGAAAAGGCGTCCGCGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTTCCGCGGCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCGTGGTTT‑GTAGGCCGGATAAGGCGTTCACGCCGCATCCTGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCTTCTGCTCCCATCGTGCGtt                                                 <  2:194884/248‑1 (MQ=255)                                                                                                                                                                                                       atacTAAGGTTGTTGTAGGCGGAAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCtt                                     <  1:52066/248‑1 (MQ=255)                                                                                                                                                                                                         acTAAGTCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTc                                    <  2:236371/249‑1 (MQ=255)                                                                                                                                                                                                            aaGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTAc                                <  1:119861/251‑1 (MQ=255)                                                                                                                                                                                                               gCTGTTGTAGGCGGGAAAAGCCGTTCCCGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTTCCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCTGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTca                             <  2:25981/251‑2 (MQ=255)                                                                                                                                                                                                                      taggccGGAAAAGGCGTTCACGCCGTATCCGGAATTCAGTGCTCACTGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGATTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGACCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGt                      >  2:17923/5‑251 (MQ=255)                                                                                                                                                                                                                       aggccgaaaaaggcgtTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGAAGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATACACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGt                      <  1:5586/236‑1 (MQ=255)                                                                                                                                                                                                                                aaggcgtTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGCTTCACAACGTATTGAAATTTC‑TTGGTTTGTCGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCga             >  1:163896/6‑250 (MQ=255)                                                                                                                                                                                                                                          cgccgtATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTC‑TTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTCTGCGCCCAGCGTGCGTGTCTTGTACTCTTCGTACTCAGTAAAGTTACCTTCGAAGAACTCAACt  <  2:264393/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       ATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGC‑TGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGCGCCCAGCGTGCGTTTCTTGTACTCTTCGTACTCGGTAAAGTTACCTTCGAAGAACTCAACT  >  NC_000913/4628483‑4628966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑