Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,628,668	C→T	intergenic (+121/+187)	nadR → / ← ettA	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,628,668	0	C	T	100.0%	85.6 / NA	29	intergenic (+121/+187)	nadR/ettA	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/7);  total (22/7)

AGAACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCTGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGC  >  NC_000913/4628447‑4628905                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              agaACAATATCGAATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATAAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCtgttgt                                                                                                                                                                                                                   >  1:74131/1‑249 (MQ=255)     cAATATCGGATTCGTGCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGCGCTGGTGCGGGAGATGCTGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAACGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGCGGCTGTCGGGATTAAACAAGGTTTAAAAAACCCTGCACGGGTGACCCGCTACGAACTTGCGGATACTAAGGCTGTTGTAGTCCg                                                                                                                                                                                                             >  2:32893/1‑251 (MQ=255)            gaattcgggCGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGaaaagg                                                                                                                                                                                                      <  1:241303/243‑4 (MQ=255)                     cGGGTTGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCAcgc                                                                                                                                                                                             >  1:30078/1‑251 (MQ=255)                          tGAAGAGGACGATTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCGCGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCAcgccgta                                                                                                                                                                                         <  1:21223/250‑3 (MQ=255)                                     aTTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCgg                                                                                                                                                                             >  1:67974/1‑251 (MQ=255)                                     aTTATGACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACATCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGCGATCCGCTACGAAATTGCGGCTACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCgg                                                                                                                                                                             >  2:76106/1‑251 (MQ=255)                                      ttatgACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGt                                                                                                                                                                            >  1:236371/5‑251 (MQ=255)                                          gACAGCCGTTTCCTGCGCTGCGTGGAGCTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGGGATGAAACGGCTTAAAGGCGAGGTAAAAAATAAGTTTTTG‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCTTTCACGCCGTATCCGGCATTCGGTGCt                                                                                                                                                                         <  2:101854/250‑1 (MQ=255)                                                                  gagcTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGCGATGAAACGGCTCACAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGCTGCGACGCTTACcgc                                                                                                                                                >  2:98574/4‑251 (MQ=255)                                                                            gggAGATGCTGGGGGAGCAGAGATAACCGCGATGAAACGGCTCAAAGGCGAGGTATACAATAAGTTTTTT‑GATGAGTTGCAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCttctc                                                                                                                                        >  2:161325/1‑246 (MQ=255)                                                                                                   tAACCGCGATGAAACGGCTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCCTCTTATCAGGCCtggagattcacaccgtattg                                                                                                               >  2:77185/1‑232 (MQ=255)                                                                                                               aacggCTCAACGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGCTTCACAACGTATTGAAAtttcttggt                                                                                                   >  2:52066/5‑244 (MQ=255)                                                                                                                    cTCAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAAtttcttggtttgt                                                                                               >  2:69036/1‑239 (MQ=255)                                                                                                                      cAAAGGCGAGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAgg                                                                                            >  1:204194/1‑251 (MQ=255)                                                                                                                              aGGTATAAAATAAGTTTTTT‑GATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATa                                                                                     >  2:119861/1‑250 (MQ=255)                                                                                                                                       aataagttttttGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTc                                                                             >  1:61836/7‑250 (MQ=255)                                                                                                                                             ttttttGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCAcgccgc                                                                      >  1:25981/1‑251 (MQ=255)                                                                                                                                                       aGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACACCGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAtt                                                            >  2:72598/1‑251 (MQ=255)                                                                                                                                                        gTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTGTTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAttt                                                           >  1:46514/1‑251 (MQ=255)                                                                                                                                                        gTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAGGGCGTTCACGCCGCATCCGGCAttt                                                           >  1:200834/1‑251 (MQ=255)                                                                                                                                                                       ggaggagGCTGACGAGATAAACCAAGGTTTGAAAAAACCTCCACGGGTGCCCCGCTACGAAATTGCGGATACTAAGGCGTTTGTAGGCCGGAAAAGGCGTTCCCGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAGGGCGTTCACGCCGCATCCGGCATTTTACGCATTACtt                                               <  2:200834/244‑1 (MQ=255)                                                                                                                                                                             ggcgtcagagaATAAACAAGGTTTGAAAAACCCTGCACGGGGGACCCGCTACGAAATTGCGGAAACTAAGTCTGTGGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACg                                      <  2:121243/244‑1 (MQ=255)                                                                                                                                                                               cTGTCGCGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGTCGCATCCGGCATTTTACGCATTACTTCGCAATACGCt                                    >  1:213514/1‑251 (MQ=255)                                                                                                                                                                                   cGAGATTAAACAAGGTTTAAAAAACCCTGCACGGGTGACCCGCTCCGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGt                                 <  1:72598/250‑1 (MQ=255)                                                                                                                                                                                            aCAAGGTTTGAAAAAACCTACAGGGGTGACCCCCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACg                       <  2:46514/251‑1 (MQ=255)                                                                                                                                                                                                              tGCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGTGCGTc     >  2:73289/1‑251 (MQ=255)                                                                                                                                                                                                               gCACGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAAAGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCCGTGCGTCt    >  2:106752/1‑251 (MQ=255)                                                                                                                                                                                                                 aCGGGTGACCCGCTACGAAATTGCGGATACTAAGGCTGTTGTAGGCCGGAAACGGCGTTCACGCCGTATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTGGAGATTCACAACGTATTGAAATTTCTTGGTTTGTAGGCCGGATAAGGCGTTCACGCCGCATACGGCCTTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCCGTGCGTCTgc  >  2:95064/1‑251 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              AGAACAATATCGAATTTGTGCGGGTTGAAGAGGAAGATTACGACAGTCGTTTCCTGCGCTGCGTGGAACTGGTGCGGGAGATGATGGGGGAGCAGAGATAACCGTGATGAAACTGCTCAAAGGCGAGGTATAAAATGAGTTTTTTTGATGAGTTGAAAACCTCTCTGGAAGAGGCTGTCGAGATTAAACAAGGTTTGAAAAAACCTGCACGGGTGACCCGCCACGAAATTGAGGATGCTAAGGCTGTTGTAGACCGGAAACGGTGTTCACGCCGCATCCGGCATTCGGTGCTCAATGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACAGTTTTACAACGTATTGAAATTGCTGGTTTTGTAGGCCGGATAAGGCGTTCGCGCCGCATCCGGCATTTTACGCATTACTTCGCAATACGCTTGTACTTGATACGCTTCGGCTCCAGCGCGTCTGC  >  NC_000913/4628447‑4628905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑