| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,628,244 | C→T | T310T (ACC→ACT) | nadR → | DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,628,244 | 0 | C | T | 96.4% | 83.3 / NA | 28 | T310T (ACC→ACT) | nadR | DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (16/11); minor base G (0/1); total (16/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
TTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTCGAAGAGAACAATATCGAATTTGTGCGGGTT > NC_000913/4628003‑4628472 | ttGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGGTTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGGAACTGATTTTGTc > 1:249719/1‑251 (MQ=255) tacGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCAACCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGCTTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCCGAATTCCGCTTACGATAAAATCGCGCTGGGCCACGCACCATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTcact > 1:159911/3‑250 (MQ=255) gATCCTTGGGGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAGTATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGGCTTTTCACACCTCGGCGGAGATGAGATCGCATTGCAGTGTTCCGATTACGATAAAATCGCGCTGGGCCCCGCACAATACATTGATTTTGCAGTGAAATATGCCCATAGAGTGGCGTTTCTTGACACTGATTTTGTCACTCCCCAGGCGTTCt > 2:9285/1‑251 (MQ=255) aGCGGTAAATCCACCCTGGTACACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCg > 1:194884/1‑251 (MQ=255) gCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCgt < 1:69449/251‑2 (MQ=255) gTAAATCCACCCTTGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCgtgag < 1:23220/251‑5 (MQ=255) tAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCCGGCGGTCTGCAAAAAGTACGAAGGGCgtgagg > 1:47777/1‑246 (MQ=255) caaacTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCAACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGgcgt > 2:98432/1‑250 (MQ=255) caataccaccAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCCATAAAGTGGCGTTTATTGACACTGATTTTg > 2:137646/5‑173 (MQ=255) caataccaccAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTg < 1:137646/169‑1 (MQ=255) aGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCAACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGt < 1:98432/251‑1 (MQ=255) cGTGGGAATATGGTCGCGATTATGTCTTTTCACTCCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGTGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATc < 1:131525/251‑1 (MQ=255) gggAATATTGCCGCGATTATGTCTTGTCACACCTCGGCTGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATTCCAATAAAGTGGCGTTTATTGACGCTTATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCTTCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCctg < 1:154004/251‑1 (MQ=255) aTGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGGGAACAACACGCCg > 1:114163/1‑251 (MQ=255) ttttCACACCTCGGCGGTGATGAGATCGCCTTGCAGTATTTCGATTACGATAACATCGCGCTGGGCCCCGCACCATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCCCTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGGTTCGATCTGGGGATCCTGCTGGAGAACCACACGCCgtgggg > 2:241303/1‑250 (MQ=255) cacaCCGCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGc < 2:77642/250‑1 (MQ=255) cggcggTGATGAGGTCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGGCACGCCCCATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTTCAAAAAGTAAGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGCTGGtt > 2:98672/1‑251 (MQ=255) tCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGTGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGATGGAGAACAACACGCCGTGGGTGGCGGTTGGTTTAAGCAGCCTctgc > 2:5586/1‑248 (MQ=255) attccgattACGATAAAATCGCGCTGGGCCACGCACAATACAGTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGAt < 1:9285/243‑1 (MQ=255) attACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTAACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCaa > 1:138130/3‑250 (MQ=255) gactaTAAAATCGCGCTGGGCCACGCACAATACATGGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACGGAGTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCGGCTGGAGAACAACACGCCGTGGGTGGCGGAAGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAaga < 2:245919/247‑1 (MQ=255) aaaaTCGCGCTGGGCCACGAACAATACATTGATTTTGCATTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACGCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTTATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGTCTCTGCAGTTCGGTGGATCGCAAACAGTTcc < 2:166949/250‑1 (MQ=255) cAATACATTGATTTTGCAGTGACATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACCCGCCGTGGGGGGCGGATGGTTTACGCAGCCTCGGAAGTTCGGTGGATCGCAACGAGTTCCAGAACTTGCTGGGGGAGAGGCt > 2:192368/1‑251 (MQ=255) cAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCt > 1:92297/1‑251 (MQ=255) aTACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCtgg > 1:121243/1‑249 (MQ=255) gCCAATAAAGTGGCGTTTATTGACACTGATTTTTTCACTACCCAGGCGTTCTGCAAAAAGGACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGc < 2:47777/250‑1 (MQ=255) aaTAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGt > 1:210024/1‑251 (MQ=255) aTAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGGTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGtt < 2:114163/251‑1 (MQ=255) | TTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTCGAAGAGAACAATATCGAATTTGTGCGGGTT > NC_000913/4628003‑4628472 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |