Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,628,238	C→T	I308I (ATC→ATT)	nadR →	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,628,238	0	C	T	100.0%	87.2 / NA	28	I308I (ATC→ATT)	nadR	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/12);  total (16/12)

TTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTCGAAGAGAACAATATCGAATTTGTGCGGGTT  >  NC_000913/4628003‑4628472                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           ttGTACGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGGTTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGGAACTGATTTTGTc                                                                                                                                                                                                                             >  1:249719/1‑251 (MQ=255)    tacGTACCGTGGCGATCCTTGGTGGCGAGTCGAGCGGTAAATCCAACCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGCTTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCCGAATTCCGCTTACGATAAAATCGCGCTGGGCCACGCACCATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTcact                                                                                                                                                                                                                          >  1:159911/3‑250 (MQ=255)                 gATCCTTGGGGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAGTATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGGCTTTTCACACCTCGGCGGAGATGAGATCGCATTGCAGTGTTCCGATTACGATAAAATCGCGCTGGGCCCCGCACAATACATTGATTTTGCAGTGAAATATGCCCATAGAGTGGCGTTTCTTGACACTGATTTTGTCACTCCCCAGGCGTTCt                                                                                                                                                                                                             >  2:9285/1‑251 (MQ=255)                                    aGCGGTAAATCCACCCTGGTACACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCg                                                                                                                                                                                          >  1:194884/1‑251 (MQ=255)                                     gCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCgt                                                                                                                                                                                         <  1:69449/251‑2 (MQ=255)                                        gTAAATCCACCCTTGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCgtgag                                                                                                                                                                                      <  1:23220/251‑5 (MQ=255)                                         tAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCCGGCGGTCTGCAAAAAGTACGAAGGGCgtgagg                                                                                                                                                                                     >  1:47777/1‑246 (MQ=255)                                                           caaacTTGCCAATATCTTCAATACCACCAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCAACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGgcgt                                                                                                                                                                   >  2:98432/1‑250 (MQ=255)                                                                             caataccaccAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCCATAAAGTGGCGTTTATTGACACTGATTTTg                                                                                                                                                                                                                               >  2:137646/5‑173 (MQ=255)                                                                             caataccaccAGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTg                                                                                                                                                                                                                               <  1:137646/169‑1 (MQ=255)                                                                                       aGTGCGTGGGAATATGGTCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCAACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGt                                                                                                                                       <  1:98432/251‑1 (MQ=255)                                                                                           cGTGGGAATATGGTCGCGATTATGTCTTTTCACTCCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGTGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATc                                                                                                                                   <  1:131525/251‑1 (MQ=255)                                                                                              gggAATATTGCCGCGATTATGTCTTGTCACACCTCGGCTGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATTCCAATAAAGTGGCGTTTATTGACGCTTATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCTTCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCctg                                                                                                                                <  1:154004/251‑1 (MQ=255)                                                                                                                aTGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGGGAACAACACGCCg                                                                                                              >  1:114163/1‑251 (MQ=255)                                                                                                                     ttttCACACCTCGGCGGTGATGAGATCGCCTTGCAGTATTTCGATTACGATAACATCGCGCTGGGCCCCGCACCATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCCCTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGGTTCGATCTGGGGATCCTGCTGGAGAACCACACGCCgtgggg                                                                                                         >  2:241303/1‑250 (MQ=255)                                                                                                                         cacaCCGCGGCGGTGATGAGATCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGc                                                                                                      <  2:77642/250‑1 (MQ=255)                                                                                                                                cggcggTGATGAGGTCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGGGGCACGCCCCATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTTCAAAAAGTAAGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGCTGGtt                                                                                              >  2:98672/1‑251 (MQ=255)                                                                                                                                              tCGCATTGCAGTATTCCGATTACGATAAAATCGCGCTGTGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGATGGAGAACAACACGCCGTGGGTGGCGGTTGGTTTAAGCAGCCTctgc                                                                                >  2:5586/1‑248 (MQ=255)                                                                                                                                                          attccgattACGATAAAATCGCGCTGGGCCACGCACAATACAGTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGAt                                                                    <  1:9285/243‑1 (MQ=255)                                                                                                                                                                attACGATAAAATCGCGCTGGGCCACGCACAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTAACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCaa                                                               >  1:138130/3‑250 (MQ=255)                                                                                                                                                                  gactaTAAAATCGCGCTGGGCCACGCACAATACATGGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACGGAGTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCGGCTGGAGAACAACACGCCGTGGGTGGCGGAAGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAaga                                                            <  2:245919/247‑1 (MQ=255)                                                                                                                                                                        aaaaTCGCGCTGGGCCACGAACAATACATTGATTTTGCATTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACGCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTTATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGTCTCTGCAGTTCGGTGGATCGCAAACAGTTcc                                                       <  2:166949/250‑1 (MQ=255)                                                                                                                                                                                             cAATACATTGATTTTGCAGTGACATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACCCGCCGTGGGGGGCGGATGGTTTACGCAGCCTCGGAAGTTCGGTGGATCGCAACGAGTTCCAGAACTTGCTGGGGGAGAGGCt                                 >  2:192368/1‑251 (MQ=255)                                                                                                                                                                                             cAATACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCt                                 >  1:92297/1‑251 (MQ=255)                                                                                                                                                                                               aTACATTGATTTTGCAGTGAAATATGCCAATAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCtgg                               >  1:121243/1‑249 (MQ=255)                                                                                                                                                                                                                        gCCAATAAAGTGGCGTTTATTGACACTGATTTTTTCACTACCCAGGCGTTCTGCAAAAAGGACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGc       <  2:47777/250‑1 (MQ=255)                                                                                                                                                                                                                           aaTAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGTTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGt   >  1:210024/1‑251 (MQ=255)                                                                                                                                                                                                                            aTAAAGTGGCGTTTATTGACACTGATTTTGTCACTACCCAGGCGGTCTGCAAAAAGTACGAAGGGCGTGAGCATCCGTTCGTACAGGCGTTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTGGAAGAGAACAATATCGAATTCGTGCGGGtt  <  2:114163/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           TTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAATCCACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGTGATCCTGCTGGAGAACAACACGCCGTGGGTGGCGGATGGTTTACGCAGCCTCGGCAGTTCGGTGGATCGCAAAGAGTTCCAGAACTTGCTGGTGGAGATGCTCGAAGAGAACAATATCGAATTTGTGCGGGTT  >  NC_000913/4628003‑4628472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑