| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,627,518 | C→G | V68V (GTC→GTG) | nadR → | DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,627,518 | 0 | C | G | 96.5% | 73.8 / NA | 29 | V68V (GTC→GTG) | nadR | DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (9/19); minor base T (0/1); total (9/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
TAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGT > NC_000913/4627273‑4627762 | tAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCAGCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTa < 1:262005/249‑1 (MQ=255) cGTGTTCGTCGACTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTAGTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTGTGGGGGCTTGAGTTTCCCCTGCAGAAGAAAGCCATTGGTGTTGTATTCgg < 2:174006/251‑1 (MQ=255) acTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTAcc < 1:80275/251‑1 (MQ=255) cTTATAATGAGGAATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTAGGTAAGTTCTAcac > 1:54259/1‑249 (MQ=255) aggaataCGGAGGGCGATATGACGTAATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCGGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGAACAAAGGGTATTTACGCCAGTTCCTGAATGCCAAAATCAAAAGCCCCAGCGAGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAACCCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATAc > 2:152736/5‑251 (MQ=255) gaataCGGAGGGAGATATTTCGTTATTTGATTACCTGAAAACTGCCATCAAGCAACAGAGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCg < 1:18514/249‑1 (MQ=255) gaataCGGAGGGAGATAGGTCGTCATTTGAGTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTGCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCTGTAAGTTCTACCCGCTGCATACCg < 2:252294/249‑1 (MQ=255) aataCGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCgg < 2:91523/250‑1 (MQ=255) ttGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGcc > 1:197469/1‑251 (MQ=255) cTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGGATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATCTCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGAc > 2:79857/1‑251 (MQ=255) gccataacgCAACAGGGATGCGCGCTACAGCAGGCGGCTGAACCCAGCGGGATGCCCATCGGGGATTTAAGCCCGATACTACCTGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCCGGAGGCGCTGCACCGTTTTTTGGGGCTTGAGATTCCCAGACAGCAGAAAACCATTGGCGAGGAATTTGGTAAGTTCTACCCGCTGCCTAACGGACGTATCTACCTTATCCAGCGACCCTGTTGCCAGGTTGACg > 2:117632/9‑250 (MQ=255) cATCAAGCAACAGGGCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGaac < 1:120269/251‑3 (MQ=255) gggCTGCACGCTACAGCAGGTGGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGTAGGCGTTGCACCTTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATGCGGTAAGTTCTACCCGCTGCATACCGGACATATATACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGACCTGCATATCagta < 2:117287/251‑4 (MQ=255) gCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATcgcg < 1:29019/250‑1 (MQ=255) ttAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTc > 1:201051/1‑251 (MQ=255) gAATGCCATAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGCTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCGTATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTTTTTGAAGACAGCGTTATGTCGCAGCAGcccac < 1:186850/249‑4 (MQ=255) gCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCGTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGct < 1:72463/251‑2 (MQ=255) aTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCg < 1:70696/251‑1 (MQ=255) gcgcAAAAGCTGGAGGCTTTGCACAGTTTGTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACAATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCAGTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCGGCGCTGGGTAtt < 2:164934/251‑1 (MQ=255) cAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTAttaca < 2:168052/251‑4 (MQ=255) tGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCCTTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGCTACCCgcgatc > 1:23715/1‑160 (MQ=255) tGCACCGGTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTACTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTGATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGTTTGTGACTATACCCgctatc < 2:23715/165‑6 (MQ=255) ggggCTTGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGc > 2:176881/1‑250 (MQ=255) tGAGTTTCCCCGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCa < 1:142292/249‑1 (MQ=255) ccccGGCAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCa < 2:201051/250‑1 (MQ=255) cAGAAGAAAACCATTGGTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAaga < 1:251701/251‑1 (MQ=255) ccattggTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAg < 1:6307/246‑1 (MQ=255) ggTGTGGTATTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACTGCATATCATTATGGGTTTTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCTGCGCTGGTTATTACAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGt > 1:38039/1‑250 (MQ=255) ggTGTGGTAGTCGGTAAGTTCTACCCGCTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAACGGCATAGCATTATGGGTTGTGACGATACCCGCGATCGCGCGTTGTTTGAAGACAGCGCTATGTCGCAGCAGCCCACCGTGCCGGATCGTCGGCGCTGGTTAttac < 2:130557/187‑3 (MQ=255) | TAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGCACGCTACAGCAGGTAGCTGATGCCAGCGGTATGACCAAAGGGTATTTAAGCCAGTTACTGAATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATCAGAAAAATATTCGCATTCATGCTTTCAACGAAGAGGGCATGGAGCCGT > NC_000913/4627273‑4627762 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |