Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,627,069	C→T	R386R (CGC→CGT)	radA →	DNA recombination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,069	0	C	T	96.0%	146.2 / NA	50	R386R (CGC→CGT)	radA	DNA recombination protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (20/28);  minor base G (1/1);  total (21/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAACCCACGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTGTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGAT  >  NC_000913/4626831‑4627314                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      cgcggccgttgctgGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGATCGCTg                                                                                                                                                                                                                                           <  2:13818/242‑1 (MQ=255)    gtccgttgctgGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCCAGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGccc                                                                                                                                                                                                                                        >  2:234857/7‑250 (MQ=255)     tccgttgctgGTGGAGATTCAGGCGCTGGTCGATCACTCGATGCTGGCGCATCAGCGAAGCGTGGCAGTGGCGCTGGCACAAAACCGTCTGGCACTCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCCAATGGCCGAACAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGAAACCGGAACCAGCGCCGACTTAGCGGTAATGCTGGCGATGGTTTCCAGCCTGCGTGACCGGCCGCTGccac                                                                                                                                                                                                                                       >  2:242046/6‑249 (MQ=255)      cagttgctgGTGCAGATTCAGGCGAGCGTCGAGCACTCGATGATTGCGAATCCGCGCCACGTGGCAGTTGGGCTGGAACAACACCGTCGTGCAAGCCGGCTGGCTGTCGTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTGGGTGGCGGGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTCCTGGCGATGGTTTCCAGCCTGCTTGACAGACCGCTgactc                                                                                                                                                                                                                                       <  2:16063/246‑5 (MQ=255)        gttgctgGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTATTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAgg                                                                                                                                                                                                                                    >  1:152773/3‑251 (MQ=255)            ctgGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGAGGTCTGCAAATGGCCGATCAGGATGGGTTTGTGACCGTGTTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGAGTTACTGCTGGCGGTGGTTTCAGGCCTGCGGGACAGACCGCTGCCCCAGGGTCt                                                                                                                                                                                                                                >  2:42788/1‑251 (MQ=255)                  gagaTTCAGGCGCTGGTCGATCACTCGATGATGGCGAAGCCGCGCCGCGTGGCAGTGGGGCGGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTggtggt                                                                                                                                                                                                                          <  2:200690/251‑1 (MQ=255)                                ggTCGATCACTCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTc                                                                                                                                                                                                             <  1:259074/250‑1 (MQ=255)                                                           gctgcgcgTGGCTGTGTGGCTGGTACAAACCCTTCTGGCAATCGTGCTGTCTGTCGTGCGCCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTATCCGAATCCAGCGCCGACTTAGCGTTACTGCTGGCGATGTTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGACCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGAAGATCCGCCCGGTGc                                                                                                                                                                                 <  2:150488/247‑1 (MQ=255)                                                              ccgcGTGGCGGTGGGGCTGGAACAAAACCGGCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCa                                                                                                                                                                              <  2:64895/251‑1 (MQ=255)                                                                  gTGGCAGTGGGGCTGGAACCAAACCGTCCGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGCTCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGCAACCAGCGCCGCCTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGGGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCCGCgg                                                                                                                                                                          >  2:172839/1‑251 (MQ=255)                                                                     gCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGACGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTc                                                                                                                                                                        >  1:117287/1‑250 (MQ=255)                                                                            ggCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCCCGGTGGTCTGCAAATGGCCGCTCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGCCCGCTGCCACAGGATCTGGGGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCAGGGGCCCGGAGGTCAGGaccg                                                                                                                                                                 >  2:262005/1‑247 (MQ=255)                                                                                   aCAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAAtttc                                                                                                                                                           >  1:166032/1‑246 (MQ=255)                                                                                     aaaaCCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAAtttcaga                                                                                                                                                        >  2:221967/1‑244 (MQ=255)                                                                                        aCCGTCTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAgcg                                                                                                                                                    >  1:10762/1‑251 (MQ=255)                                                                                          cGTCTGGCAATCCTGCTGGCTGGCTTGCACCGTCACTGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAgcggc                                                                                                                                                  <  2:82455/251‑1 (MQ=255)                                                                                             cTGGCAATCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGa                                                                                                                                                >  1:115028/1‑250 (MQ=255)                                                                                                    tCCTGCTGGCTGTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGt                                                                                                                                        >  1:131679/1‑251 (MQ=255)                                                                                                        gctggctgTCTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTc                                                                                                                                    <  1:218039/251‑1 (MQ=255)                                                                                                                  ttGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGAt                                                                                                                          >  1:216232/1‑251 (MQ=255)                                                                                                                    gCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGAtt                                                                                                                         <  1:96571/250‑1 (MQ=255)                                                                                                                                       cAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTGCCGGCGGCTAACGtacc                                                                                                     <  1:221967/251‑4 (MQ=255)                                                                                                                                        aaaTGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCg                                                                                                    <  2:217413/251‑1 (MQ=255)                                                                                                                                                   cAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGC‑ACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGAGCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGGTTGTTCCGGCGGCTAACGTTCCGAAAAAAGCGCCg                                                                                        <  1:76894/251‑1 (MQ=255)                                                                                                                                                    aGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCg                                                                                        <  1:244410/251‑1 (MQ=255)                                                                                                                                                            tttGTAAAAGTGGTCGGCGGCGTGCAGGTAACCGCAAACAGAGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGACACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTACAGAAGCGGCGAAACACGGCTTTAGCCGGGAGATAGTTCCGGCGACTAACGTAACGAAAAAAgacacggaaggggt                                                                                >  2:186850/1‑238 (MQ=255)                                                                                                                                                                   ccGTGGTCGGCGGCGTGAAGGTAACCGAACCCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGAtt                                                                         <  2:166032/250‑1 (MQ=255)                                                                                                                                                                      tGGTCGGCGGCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGAttttt                                                                      >  1:79245/1‑251 (MQ=255)                                                                                                                                                                       gggcggcggCGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTGACTGCTGGCGATGGTTTCCAGCCTGCGGGACAGACCGCTGCCACAGGATCTGGTGGTGGTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGTCCGGGGGCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTGCGGCGTCTAACGGACCTAAAAAAGCGCCGGAAGGGATGCAGAttgtt                                                                      <  2:243160/247‑4 (MQ=255)                                                                                                                                                                         gcggcggCTTGAATGTAACCGAATCCTGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTTACAGACCTCTGCCACAGGATCTGGTGGTGTGTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCATATTTTTGGc                                                                   <  1:96942/250‑1 (MQ=255)                                                                                                                                                                           ggcggcGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGt                                                                 >  1:57114/1‑251 (MQ=255)                                                                                                                                                                           ggcggcGTGAAGGTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGt                                                                 >  2:165583/1‑251 (MQ=255)                                                                                                                                                                                 gTGAGGGTAACCGAATCCAGCGCCGACTTAGCGTTACTGCTGGCGAGGGTTTCCAGGCTGCGTGACGGACCGCTGCCACAGGAGCTGGTGGTGTTTGGTGAAGTCGTGTTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAAGTTCAGAAGCGGCGAAACACGGTTTTCGCGGGGGGATTGTTCCGGCGGCGAACGTACCGAAAAAATCGCCGGAAGGGATGCAGAGTTGTGGCGGTaaaaa                                                           <  2:34466/251‑1 (MQ=255)                                                                                                                                                                                      ggTAACCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTAGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTAAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACAGAAAAAAGCGCCGGAAGGGATGCAGAATTTTGGAGTTAAAAAActct                                                      >  2:72463/1‑251 (MQ=255)                                                                                                                                                                                         aaCCGAAACCCGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGACACAGGATCGGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGCTCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCg                                                   <  2:152773/251‑1 (MQ=255)                                                                                                                                                                                         aaCCGAAACCAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGTTCAGGAACGAATTTCAGAATCGGCGAAACACGGTTTGCGCCGGGGGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCg                                                   <  1:242046/251‑1 (MQ=255)                                                                                                                                                                                            cGAAACCAGCGCCGGCTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAATCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGAcg                                                <  1:228019/251‑1 (MQ=255)                                                                                                                                                                                            cGAAACAAGCGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAACGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGAcg                                                >  2:235801/1‑251 (MQ=255)                                                                                                                                                                                                     cgCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCg                                       <  2:115028/250‑1 (MQ=255)                                                                                                                                                                                                        cGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCgtgt                                    <  1:234857/251‑1 (MQ=255)                                                                                                                                                                                                        cGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCgtgt                                    >  1:91523/1‑251 (MQ=255)                                                                                                                                                                                                        cGACTGAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCgtgt                                    <  1:172839/251‑1 (MQ=255)                                                                                                                                                                                                         gACTTAGCGTGACTGCTGGCGAGGGTTTCCAGCCTGCGTGACAGTCCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGtt                                   <  2:76199/251‑1 (MQ=255)                                                                                                                                                                                                             tAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTcg                                 <  1:128559/249‑1 (MQ=255)                                                                                                                                                                                                              aGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGCCCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGTCTGGCAGGGTAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTcgacg                              <  1:120068/251‑1 (MQ=255)                                                                                                                                                                                                                  ttACTGCTGGCGAGGGTTTCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCgacgac                            <  2:10762/249‑1 (MQ=255)                                                                                                                                                                                                                                   ttCCAGCCTGCGTGACAGACCGCTGCCACAGGATCTGGTGGGGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGTGATGCATATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCTTGTTCGACGACATATAAttgggaatacgg          <  2:183409/250‑12 (MQ=255)                                                                                                                                                                                                                                         ccTGCGTGACAGACCTGTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGgaga   <  1:103437/251‑1 (MQ=255)                                                                                                                                                                                                                                           tGCGTGACAGACCGCTGCCACAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATTTCAGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGGAATACGGAGGGAGat  >  2:29019/1‑250 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      CGCGTCCACTGCTGGTGGAGATTCAGGCGCTGGTCGATCACTCGATGATGGCGAACCCACGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTGTTGCACCGTCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTGAAGGTAACCGAAACCAGTGCCGACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGCGACAGACCGCTGCCGCAGGATCTGGTGGTGTTTGGTGAAGTCGGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGCGATTGTTCCGGCGGCTAACGTGCCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGAT  >  NC_000913/4626831‑4627314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑