Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,132	A→C	G276G (GGT→GGG)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,132	0	A	C	100.0%	46.8 / NA	21	G276G (GGT→GGG)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/7);  total (14/7)

ACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGC‑GCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCG  >  NC_000913/6916‑7377                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      aCCCCAGGCACCAATTAACCCCCGCATCGCCTCCTGGATAAGCTGGATCCCTTCCAGCGGCCTGTAGGTTGTGCCGTTACCCGCCAGAAATCTCAGCATGGCGCTTGCCGTACAGATGACCAGGGAGTCGATCAACATGCCAAACATCTGGACAATCCCTTGC‑GCTGCCGGA‑TGCGGAGGCCAGGACGCCGCCGCCGCTGCCGCGTTTGGCGCCGCCCCCATTCCCGCCTCATTGGAAAACATACTGAGCt                                                                                                                                                                                                                     >  2:16477/1‑251 (MQ=255)                taacACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGC‑GCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAACACCGTTAGTa                                                                                                                                                                                                       >  1:51257/4‑250 (MQ=255)                                                tACCTTCCAGCGGCATGTAGGGTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGC‑GCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCcgccgcgccg                                                                                                                                                                      >  1:146679/1‑251 (MQ=255)                                                       cAGCGGCATGTAGGTTGTGCCGTTACCCCCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGCGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGC‑GCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTAGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCCCCGCCTGCCg                                                                                                                                                               >  2:264482/1‑251 (MQ=255)                                                          cGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGCCAATCCCTTGC‑GCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGGATCTCCCGCCGCGCCGCCTGCCGCtt                                                                                                                                                            >  2:31189/1‑251 (MQ=255)                                                              atgtggtttgtcccgtaccccGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAACCATCTGGACAATCCCTTGC‑GCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCCGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCt                                                                                                                                                         <  1:162903/232‑1 (MQ=255)                                                                                   gCCAGTAATATCAGCATGGCGATTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGC‑GCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCCGCCAAAAGCACTCTCaa                                                                                                                                    >  2:246582/1‑250 (MQ=255)                                                                                                       gcTTGCCGTACAGATGACCAGGGTGACGATAAAAATGCCAATCATCTGGACAATCCCTTGC‑GCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAACTAGACAAAATGACGTggg                                                                                                               >  2:200387/1‑251 (MQ=255)                                                                                                        cTTGCCGTCCAGATGACCAGGGCGTCGCTAAAAATGCCAATCATCTGGACAATCCCTTGC‑GCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTCATCGCCTGGCTTAAGGCATATCCCGCCGCGCCGCCAGCCGCTTCCTGACAGCCAACAGCACTCTCAAAAATCGACCAACTGCCGTgggg                                                                                                              >  2:162493/1‑251 (MQ=255)                                                                                                        cTTGCCGTACAGATGACCAGGGTGTCGATAAAACTGCCAATCATCTGGACAATCCCTTGC‑GCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCAGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTgggg                                                                                                              >  2:155137/1‑251 (MQ=255)                                                                                                          tGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGC‑GCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCCAAAATAGACCAAATGACGTGGGGa                                                                                                             >  2:79739/1‑250 (MQ=255)                                                                                                                 agtatgcccAGGGTGCAGAAAAAAATCCCAAACATCGGGACAA‑CCCTTGCCGC‑GCCGGGGTGCGGAGGCCAGGACCCCGCTGCCGCTGCCGCGTTTGGGGTCGCCCCAATTCCCGCCTCATTGGAAAACATACTGCGCTGAAACCCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGccc                                                                                                     <  2:11383/244‑1 (MQ=255)                                                                                                                                        aaTGCCAATCATCTGGACATCCCCTTGC‑GCTGCCGGA‑TGCGGAGGCCAGGACGCGGCTGCCGCTGCCGCGTTGGGCGTCGACCCCTTTCCCGCCTCATTGGAAAACAGACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCa                                                                              <  1:101614/251‑1 (MQ=255)                                                                                                                                                ccaccgggACAATCCCTTGC‑GTTGCCGGG‑GGGGGGGGCCGGTCCGCCGCTGCCGCTGCCGCTTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTc                                                                      <  2:222525/245‑1 (MQ=255)                                                                                                                                                          aaTCCCTTGC‑GCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTAccc                                                               >  1:38647/1‑248 (MQ=255)                                                                                                                                                                    cccggCCGGA‑TGCGGGGCCCAGGACGCCGTTGCCGCTGCTGCGTGTGGCTACGACCCCATTCCCGTCGCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAAGCGCCTGGCTTAAGGTATATCCCGCCGCGCCTCCTGCCGCTTCCTGCCAGCCAAAAGCACGCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCa                                                   <  1:244551/247‑1 (MQ=255)                                                                                                                                                                            ggaggcgggggccagacccccgctgccgctcCCGCGTTTGGCGCCGCCCCCATCCCCGCCTCATTGGAAACCATACTGCGCTGAAACCCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACgg                                            <  2:263495/232‑1 (MQ=255)                                                                                                                                                                            gA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACggg                                           >  2:197828/1‑251 (MQ=255)                                                                                                                                                                                               ccgctgccgctgCCGCGTTTGGCGTCGACCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAAtca                                                                                 >  1:14925/1‑193 (MQ=255)                                                                                                                                                                                                                   ggCGTCGCCCCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATACCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGAcc     <  1:154318/251‑1 (MQ=255)                                                                                                                                                                                                                      gTCGACCCCATTCCCGCCTCATTGGAAAACATCCTGCGCTGAAAACCGTTAGAAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCCAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATCACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAACCCCTGCATGAGCCGGGCGACGCCATGAAGACcgcg  >  1:90673/1‑251 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      ACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAATCATCTGGACAATCCCTTGC‑GCTGCCGGA‑TGCGGAGGCCAGGACGCCGCTGCCGCTGCCGCGTTTGGCGTCGAACCCATTCCCGCCTCATTGGAAAACATACTGCGCTGAAAACCGTTAGTAATCGCCTGGCTTAAGGTATATCCCGCCGCGCCGCCTGCCGCTTCCTGCCAGCCAAAAGCACTCTCAAAAATAGACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCG  >  NC_000913/6916‑7377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑