Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,626,565	G→T	V218V (GTG→GTT)	radA →	DNA recombination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,626,565	0	G	T	100.0%	104.9 / NA	34	V218V (GTG→GTT)	radA	DNA recombination protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/20);  total (14/20)

CATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAG  >  NC_000913/4626335‑4626804                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                cATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATgg                                                                                                                                                                                                                             >  1:10194/1‑251 (MQ=255)  aTCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCAGATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATggc                                                                                                                                                                                                                            <  2:39750/251‑2 (MQ=255)            ccTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCAGCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCgctggcgt                                                                                                                                                                                                                  <  2:8307/251‑3 (MQ=255)                      gACAATCTCAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTaaa                                                                                                                                                                                                          <  1:96371/249‑1 (MQ=255)                            ctcAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTgg                                                                                                                                                                                                   >  1:204839/1‑250 (MQ=255)                             tcAATATGTTGTCGGAAACCAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGa                                                                                                                                                                                                  >  1:211858/1‑250 (MQ=255)                                       gtCGGAAACAAGCATCGAGCAGAGCTTCCGGATGGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGAGTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATc                                                                                                                                                                                        <  2:131552/250‑1 (MQ=255)                                                cAGCATCGAGCAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGCTGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCAAGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGGACACTGTATCCACTGTTCgg                                                                                                                                                                              >  2:179060/1‑251 (MQ=255)                                                       gagcAGATATGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTACTTGACTCGATCCAGGTGCTGCATATGGAGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCtttt                                                                                                                                                                       >  1:255245/4‑251 (MQ=255)                                                           aGATCGGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGAt                                                                                                                                                                   <  2:10194/251‑1 (MQ=255)                                                                  ccTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATg                                                                                                                                                            <  1:101258/251‑1 (MQ=255)                                                                              agaagaGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGtt                                                                                                                                                <  1:24308/251‑1 (MQ=255)                                                                                aagaGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGtttt                                                                                                                                              <  2:146083/251‑1 (MQ=255)                                                                                 agagCAACCGAAGCTGATGTTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACTGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGTTGCTTTTGGATGGCGATGCCGACTCCCGTTTTc                                                                                                                                             <  1:212352/251‑1 (MQ=255)                                                                                     cAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATAGGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCAc                                                                                                                                         <  2:210689/251‑1 (MQ=255)                                                                                         cGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGGTTTCGCActtta                                                                                                                                     <  1:68038/251‑5 (MQ=255)                                                                                                               gATCCAGGGGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCg                                                                                                               <  2:78019/251‑1 (MQ=255)                                                                                                                 tCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGAGGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGgc                                                                                                             >  1:82455/1‑251 (MQ=255)                                                                                                                        gATGCATATGGCGGATGTACAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGTTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTGTCGCACTTTACGCAGCCAGAAAAACCGCTGCGGCGCGGTg                                                                                                       <  2:215324/250‑1 (MQ=255)                                                                                                                                          aCAGTCATCGCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTcg                                                                                    >  1:17141/1‑251 (MQ=255)                                                                                                                                                aTCGCCTGGCAGCGTGGCGCAGGTGCGTGAACCGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGCTGGCGATGCCGACTCCCGTTTTCGCACTTTACGCATCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATg                                                                               >  1:226061/1‑250 (MQ=255)                                                                                                                                                   gCCTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCg                                                                           <  1:61844/251‑1 (MQ=255)                                                                                                                                                    ccTGGCAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCCGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCg                                                                           >  2:37525/1‑250 (MQ=255)                                                                                                                                                          aGCGTGGCGCAGGGGCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAATCGCGCGGTGTGGCGATTGTCATGGTTGGACAAGTAACCAAAGCTGGCTCGCTGGCAGGCCCTAAAGGGATGGAACACTGTATCGACTGGTCTGTGATTTTGGATGGGGGTTCCGAATCCCGTTTTCGCACTTTACCCAGCCCTAAAAACCCCGTCGGAGCGGTTAATGAGCTGGGCGTCTTCGCGATGACCGacaaggg                                                                    >  2:96942/1‑245 (MQ=255)                                                                                                                                                                        gCGTGAAACGGCGGCTTATCTGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCa                                                      <  1:10317/251‑1 (MQ=255)                                                                                                                                                                                     gcTTATCTGACGCGCTTCGCCCAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGc                                         >  1:213291/1‑251 (MQ=255)                                                                                                                                                                                         atctgacgctctgcGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGTCTCGCTTGCGGGCCCTAAAGTGCTGGAACTCTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCTATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCTTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAAt                                      <  1:91502/237‑1 (MQ=255)                                                                                                                                                                                            tGACGCGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATTGCTCGATGGCGGGCCCTAAAGTGCTGGGACAATGTATCGCCTGTTCGGTGCTTTTGGATGGCGATGACGACTCCCGTTTTCGCTATTTAAGCAGCCATAAAAACCGCTTCGGCGCGGAGAATGAGCTCGGCGTCTTCGGGATGAACGAACAGGGGCTGCGTGAAGTCAGCAACCCCTCGGCACtttac                                  >  2:259074/1‑249 (MQ=255)                                                                                                                                                                                                      cGCCAAAACGCGCGGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTTGGCAATTTTCTTAAGTcgcg                        >  2:67028/1‑251 (MQ=255)                                                                                                                                                                                                                 gcgGTGTGGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTg             <  1:179060/251‑1 (MQ=255)                                                                                                                                                                                                                  cgGTGTGGCGATTGTCATGGTTAGACACGTAACCAAAGATGGGTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGCACAGGGGCAGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGGGATGagggg             >  2:120068/1‑246 (MQ=255)                                                                                                                                                                                                                      gtgGCGATTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTc        <  1:207759/251‑1 (MQ=255)                                                                                                                                                                                                                            aTTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGACCAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGTCCTCCggcag  <  1:23130/251‑4 (MQ=255)                                                                                                                                                                                                                            aTTGTCATGGTTGGACACGTAACCAAAGATGGCTCGCTGGCGGGCCCTAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACTTTACGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAGTGACCTCCggcag  <  2:128367/251‑4 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                CATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAACAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGTACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGGCGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCGGTGCTTTTGGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCTGGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGCGATGAAGTGACCTCCGGTAG  >  NC_000913/4626335‑4626804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑