Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,625,044	A→G	Q50Q (CAA→CAG)	serB →	phosphoserine phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,625,044	0	A	G	97.3%	115.5 / NA	37	Q50Q (CAA→CAG)	serB	phosphoserine phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (22/14);  minor base T (0/1);  total (22/15)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGA  >  NC_000913/4624800‑4625280                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              ttGAAAATTTGAGACTTAATGGTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCGGCTGTATGGTCGTGGGCTGGATAAACAGCGTc                                                                                                                                                                                                                                           <  2:90748/249‑1 (MQ=255)  tGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTg                                                                                                                                                                                                                                         <  1:50575/250‑1 (MQ=255)            agaCTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATAcc                                                                                                                                                                                                                               >  1:5388/1‑250 (MQ=255)              aCTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATAcc                                                                                                                                                                                                                               >  2:18433/1‑248 (MQ=255)              aCTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGATGGATAAACAGCGTCTGAACCacaccccg                                                                                                                                                                                                                             >  2:13375/1‑243 (MQ=255)                  aaTGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCaa                                                                                                                                                                                                                        <  1:18433/251‑1 (MQ=255)                         ccAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGCTGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTgggg                                                                                                                                                                                                                 >  1:13151/1‑250 (MQ=255)                            gAAGCAATGGATCCAAGGTCGCCGCATGCGTTCTTTTCCCTGCTTAGCACGATCTTCCAGGAGCCTTCAAGAATAGCATGAGCTGGTGAGACATGCCCGAAGATGTCTATTTATGGCCGGGACTGACTCTTAATTTAAGTGGAGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTCGCGGCTGGCTGCTGAATGGTCGTGGGCGGGATAAACAGCGTCTGGCCAAATCGCAGAGAAAACTGGGTgtg                                                                                                                                                                                                              >  2:132122/1‑249 (MQ=255)                               gCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTgcggcg                                                                                                                                                                                                           <  2:230331/251‑1 (MQ=255)                                cAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTgcggcg                                                                                                                                                                                                           >  1:170967/1‑250 (MQ=255)                                 aaTGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGAt                                                                                                                                                                                                         >  1:84758/1‑251 (MQ=255)                                        acatggTAGCCACATTCGTTATTTGCCCTTCGTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCGGGTGCGCCCTGCCTGAAGATGTTTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGGGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGAtt                                                                                                                                                                                                  <  2:26037/247‑1 (MQ=255)                                           aGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGAttgt‑t                                                                                                                                                                                              >  1:242563/1‑251 (MQ=255)                                                      tGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGGGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTg                                                                                                                                                                                   >  2:257025/1‑251 (MQ=255)                                                                tCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTGTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TTCCGCCTGGTTCGTGGAAGa                                                                                                                                                                          <  2:242563/250‑1 (MQ=255)                                                                tCCCTGCTTCGAACGATTGTACAGGAACCTTAATGCCTTACATTACCTGGTGCGACCGGCCTGAAGTTGCCTCTGTATGGCCGGGTCTGCCTCTGTCATTAAGTTGAGATTAAGTGATGCCACTGTATGCCCCCGCAGGTCCTAGCGGCTGGCTGCTGTATGGGCGTGGGCTGGCTAAACATCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTGCGTGGAAGa                                                                                                                                                                          <  1:132122/250‑1 (MQ=255)                                                                                         agtcTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGAGTGG‑TGCCGCCTGGTGCGTGGAAg                                                                                                                                                                           <  2:60623/221‑1 (MQ=255)                                                                                         aGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTGCGTGGAAg                                                                                                                                                                           >  1:60623/1‑224 (MQ=255)                                                                                         aGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGtt                                                                                                                                                <  2:5388/251‑1 (MQ=255)                                                                                         aGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGGCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTTTGGCAAGtt                                                                                                                                                >  2:227531/1‑251 (MQ=255)                                                                                          gccgtAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCGGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGAT‑GTGTGCTGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTtc                                                                                                                                               <  1:2870/247‑1 (MQ=255)                                                                                              tAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTtcac                                                                                                                                             <  1:242049/249‑1 (MQ=255)                                                                                                      aaCATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATTGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGTTGCGTGGAAGATTATCAGGTGATTCGGCTGGCAGGTTCACTCTCCGCa                                                                                                                                     <  2:197672/249‑1 (MQ=255)                                                                                                           tACCTGGTGCGCCCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCt                                                                                                                               >  1:39308/1‑250 (MQ=255)                                                                                                                     gACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGGTTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGc                                                                                                                    >  1:217482/1‑251 (MQ=255)                                                                                                                        ctgcctgAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGccc                                                                                                                  >  1:76687/1‑250 (MQ=255)                                                                                                                                   tGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCaac                                                                                                      >  1:268767/1‑249 (MQ=255)                                                                                                                                          ttATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATg                                                                                                <  1:112780/250‑1 (MQ=255)                                                                                                                                                   ggTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCt                                                                                      >  2:264641/1‑251 (MQ=255)                                                                                                                                                                     aggggggatgaAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTAGGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATTCCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTGCGTGGAAGAGTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCt                                                                    <  2:13151/245‑1 (MQ=255)                                                                                                                                                                              gaAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGcc                                                           >  1:3875/1‑251 (MQ=255)                                                                                                                                                                                  ggATGCCACGGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑GGCCGCCTGGTGCGTGGAAGATTATCAGGTGAGTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGTATGTCGCCCCGCGGGGGAAAAGCCCGCACCTGCGCACGCCGGGt                                                       <  2:101743/250‑1 (MQ=255)                                                                                                                                                                                               ttACCACGCAGGTCGTAACGGCTGGCTGCTGTATGGTCGTGGTCTGGATAAACAGCGTCTGCCCCAATACCAGAGCAAACTGGGTGCGGCGATGGGGATTGT‑TGCCGCCTGGTGCGTGGAAGATTATCAGGGGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGACGCGCACCTGGATGTCGCCCCGCTTGGGAACATCCCGCACCTGCGCCCGCCGGGGTTGCTggcgctgg                                          >  2:141747/1‑245 (MQ=255)                                                                                                                                                                                                    aCGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCCCCTGCGCACGCCGGGTTTGCTGGTGATGGACATg                                     >  2:11959/1‑251 (MQ=255)                                                                                                                                                                                                                              tATGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATg           >  1:225600/1‑251 (MQ=255)                                                                                                                                                                                                                               aTGGTCGTGGGCTGGATAAACAGCGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGCTTCCACCGCCATCCAGATTGCATGt          >  1:49195/1‑251 (MQ=255)                                                                                                                                                                                                                                        ggCTGGATAAACAGCGTCTGCCCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACGCGCCTGGCCCATGAAGCGCAACTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGACATGGATTCCACCGCCATCCAGATTGAATGTATtgatga  >  1:193435/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              TTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTCCCTGCTTCGAACGATTTTACAGGAGCCTTAATGCCTAACATTACCTGGTGCGACCTGCCTGAAGATGTCTCTTTATGGCCGGGTCTGCCTCTTTCATTAAGTGGTGATGAAGTGATGCCACTGGATTACCACGCAGGTCGTAGCGGCTGGCTGCTGTATGGTCGTGGGCTGGATAAACAACGTCTGACCCAATACCAGAGCAAACTGGGTGCGGCGATGGTGATTGT‑TGCCGCCTGGTGCGTGGAAGATTATCAGGTGATTCGTCTGGCAGGTTCACTCACCGCACGGGCTACACGCCTGGCCCACGAAGCGCAGCTGGATGTCGCCCCGCTGGGGAAAATCCCGCACCTGCGCACGCCGGGTTTGCTGGTGATGGATATGGACTCCACCGCCATCCAGATTGAATGTATTGATGA  >  NC_000913/4624800‑4625280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑