Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,624,622	C→T	V56V (GTG→GTA)	ytjB ←	putative inner membrane protein Smp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,624,622	0	C	T	100.0%	105.2 / NA	33	V56V (GTG→GTA)	ytjB	putative inner membrane protein Smp
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/21);  total (12/21)

CGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTC  >  NC_000913/4624381‑4624864                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   cGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGAcgc                                                                                                                                                                                                                                           <  2:68577/251‑1 (MQ=255)        cccggccaTTGGTTCGACACTCTGCTGGTTAAACTCGACGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGCGGATACGGCTTTCATCCGTTAACTGCTCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCCACGTTCAGCGTTACCTGACGCGCCAggg                                                                                                                                                                                                                                    >  2:242049/8‑251 (MQ=255)            gCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCg                                                                                                                                                                                                                                <  1:240094/251‑1 (MQ=255)              aaTTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGATCAGCGATACCTGACGCGCCAGGGTGCggg                                                                                                                                                                                                                              >  1:90748/1‑251 (MQ=255)                      cGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGttc                                                                                                                                                                                                                       <  2:95497/250‑1 (MQ=255)                       gACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGttctt                                                                                                                                                                                                                     >  2:74026/1‑251 (MQ=255)                                  ggTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGa                                                                                                                                                                                                          <  1:173256/251‑1 (MQ=255)                                         aTAGCCGCCCGCTTGTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACgtt                                                                                                                                                                                                   <  1:102977/251‑3 (MQ=255)                                           aGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCCGCTCTTCCAGCTGTGGAttccgttgt                                                                                                                                                                                                 >  1:26037/1‑244 (MQ=255)                                             ccgcccgcTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCTGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGcc                                                                                                                                                                                                                            <  2:253128/222‑1 (MQ=255)                                             ccgcccgcTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGcc                                                                                                                                                                                                                            >  1:253128/1‑222 (MQ=255)                                                  cgCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCTCtggt                                                                                                                                                                                          <  2:95098/250‑1 (MQ=255)                                                            ccGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCCGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACt                                                                                                                                                                                >  1:106115/1‑251 (MQ=255)                                                                       cAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGGTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACg                                                                                                                                                                     <  1:172441/251‑1 (MQ=255)                                                                        aGCCGGTCGCGCACTTCGACGCTTGCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACTGCTTTCAGCCGTTAACTGATCGAGGATCGCCGGAAGGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGAGTACGTTGGCGCTGGTGGTTTTGACTAAACCATGACGc                                                                                                                                                                    <  2:248956/251‑1 (MQ=255)                                                                                 cgcACTTCGACGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATTCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCTGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCAt                                                                                                                                                           <  2:10452/251‑1 (MQ=255)                                                                                          aCGCTTTCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCACGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCAc                                                                                                                                                  >  2:218954/1‑251 (MQ=255)                                                                                               ttCGCCAGAACGGGCGATAAGATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAAc                                                                                                                                              <  1:80351/250‑1 (MQ=255)                                                                                                          gggCGATAAGATCGCCGTGTTCGTCATACGCGACCGCGTCGAGGATACGGCTTTCATCCGTTAACTGAGCGAGGATCGCCTGAATGCGTTTTTCAGCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTGCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAg                                                                                                                                  <  2:245923/249‑1 (MQ=255)                                                                                                                   gATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGa                                                                                                                                                                                  >  2:17124/1‑194 (MQ=255)                                                                                                                   gATCGCCTTGTTCGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGa                                                                                                                                                                                  <  1:17124/194‑1 (MQ=255)                                                                                                                            gtgcGTCATACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGcc                                                                                                                <  2:229245/248‑1 (MQ=255)                                                                                                                                   aTACACGCCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCa                                                                                                         <  1:135058/251‑1 (MQ=255)                                                                                                                                                   aGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCGAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGttt                                                                                         <  1:31163/251‑1 (MQ=255)                                                                                                                                                      aTACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTtgt                                                                                      <  1:95943/251‑1 (MQ=255)                                                                                                                                                              ttCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGCGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTCCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACCGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGcc                                                                               >  2:50575/1‑250 (MQ=255)                                                                                                                                                                            gatagAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGAAGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAAACATGACGCTCCCTGCATGAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGAAGCCGGACTTTCAGTTTTGTGCGAGCCATCTTCCACCCtctg                                                                >  2:2870/5‑248 (MQ=255)                                                                                                                                                                            gATCGAGGATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTg                                                                >  1:230331/1‑251 (MQ=255)                                                                                                                                                                                  ggATCGCCTGAATGCGTTTTTCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAAtt                                                          >  2:90779/1‑251 (MQ=255)                                                                                                                                                                                           gAATGCGTTTGTCATCCGGCGAGTCTGTACGCATCAGCGGTGCAACTTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGAGTACGTTGTTGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCATCGCCACTAACAAGGTAAGGCAGAACATGACAATCGCTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTGTGAAAATTTGAGACt                                                   <  1:180801/249‑1 (MQ=255)                                                                                                                                                                                                   ttttCATCCGGCGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGc                                          <  1:74026/250‑1 (MQ=255)                                                                                                                                                                                                                                cggggCAACGTTCAGCGTTTCCGGACTAGTCAGGGTGCGGGCCAGGTCCTCCCGCTGTGGATTTCGTTGTCGCTGGTGGTTTTGACTAACCCATGACGCGCCCTGCATCAGCGCCACGAACAAGGCAAGACAGATCAGGACAAGCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGATCCATCTTTCACCATCTGAAAATTGGAGACTTAATGGTGCCTTAAGTAATGGATACAAGGTAGCCTAATg            <  2:131569/247‑1 (MQ=255)                                                                                                                                                                                                                                           tCAGCGTTACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGTTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTc  <  1:58676/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   CGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCGACGCTTTCGCCAGAACGTGCGATAAGATCGCCTTGTTCGTCATACACACCCGCGTCGAGGATACGGCTTTCATCCGTTAACTGATCGAGGATCGCCTGAATGCGTTTTTCATCCGGTGAGTCGGTACGCATCAGCGGTGCAACGTTCAGCGTCACCTGACGCGCCAGGGTGCGGGCCAGTTCTTCCAGCTGTGGATTACGCTGTCGCTGGTGGTTTTGACTAAACCATGACGCTCCCTGCATCAGCGCCACTAACAAGGCAAGACAGAACAGGACAATCACTGCCCGATGCAGCCGGAATTTCAGTTTTGTGCGAGCCATCTTCCACCCTTTGAAAATTTGAGACTTAATGTTGCCAGAAGCAATGGATACAAGGTAGCCTCATGCGTTATTTTC  >  NC_000913/4624381‑4624864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑