| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,624,226 | G→A | R188R (CGC→CGT) | ytjB ← | putative inner membrane protein Smp |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,624,226 | 0 | G | A | 93.9% | 82.0 / ‑5.8 | 34 | R188R (CGC→CGT) | ytjB | putative inner membrane protein Smp |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (14/17); minor base T (1/1); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01 | |||||||||||
CTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCG > NC_000913/4623985‑4624469 | ctacCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTTACAGGGGGGGCGATTGCTGCCAGCGGGGCCGTTTAccc > 2:219822/1‑251 (MQ=255) acCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAGAGGCGCTTGCTGCCAGCGGGGACGTTTATCCTg > 2:219282/1‑251 (MQ=255) cGGCATTGCGACAGAGAAACAGAACGCGCTGCGGGGCGGGCATCTGGCGAAAAATACACTACCCCACCGCCAGGGTAAAACACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTGACCCTGTagcag < 2:226647/250‑1 (MQ=255) gcgcCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTTTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCgg < 1:120080/250‑1 (MQ=255) gcgcCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCgg < 1:267869/250‑1 (MQ=255) gcCAGCGAAACAGAACGGCCTGCCTGGCGGGCATCTGGCGTAAAATACACTCCTCCCCCGCCCGGTTAAACCACGGGTCGTGAGAGTCAGAGATGCGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGCAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACCGGGAAGGCGCTTACTGCCCGCGGGTAAGTTTAccccatcgccgcaggcgcgtt > 2:20263/1‑233 (MQ=255) agaAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGGGGGCGGGTccgccc > 2:266490/1‑246 (MQ=255) cGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGGACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGGTTGcccgggaa > 2:178166/1‑242 (MQ=255) atctGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTTCCAGTGAAAgcagcac > 1:245923/4‑250 (MQ=255) ggCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCGTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATc < 1:178166/251‑1 (MQ=255) gAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATccgg > 1:38959/1‑248 (MQ=255) aaagacacTCCTCCTCCGCCAGGTTAAACCACGGTTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTCTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCATCCGGCAGCGGTGTGCTGGCGGTTAAAAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTTCCCTGTAGCAGCGTGCGGGGCAGCACTAAGCCGATTGCCAGTGATAGCAGCACCATTAGGCGt < 2:230059/247‑1 (MQ=255) ccccccccGCCAGGTTAAACCACCGGTCGTAAGAGTCAGAGACGAGCAGGCGTAATGTGGACCTAACGATGTCCTTTCTTGTAGTAATGGGTCACTCGTTTTTCTCGCTTTCTTCCGCTTCCGGCACCGGTTTTCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCGGCGTGCGGGTCAGTACAACTCCGATTGGCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTa < 2:49306/245‑1 (MQ=255) ccACCGCCAGGTTAAACCACGGGTCGTAAGAGGCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAgtg < 2:184823/251‑1 (MQ=255) cGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCg < 1:182770/189‑1 (MQ=255) cGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGGACGTTTACCCTGTAGCAGCGGGCg > 2:182770/1‑189 (MQ=255) tAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGGACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTAAGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTCAAATGTTAGTGGGGTTATCCAcc > 1:95098/1‑251 (MQ=255) aCGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGGTATCCACCTGTTgg < 1:219822/251‑1 (MQ=255) ggTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTTCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCt < 2:247931/251‑1 (MQ=255) aGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTc < 1:189357/251‑1 (MQ=255) ggACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTGTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGGATGGGGGTCGAGCGTCAgg < 2:38959/250‑1 (MQ=255) cGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTTACAGGAAGGGCGATTGCTGCCAGCGGGTAAGTTTTCCCTGTAGCAGCGGGCGGGGTAGCACTACGCCGATTTGCAGTGAAAACCGCAACATCAGGCGGAAAATGTTAGGGGGGTTATTCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGGCGAGCGTCAggggacgata > 2:240094/1‑242 (MQ=255) tttCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTACCAGGAAGGGCGATTTCTGGCAGCGGGTTCGTTTACCCTGTCGCAGGGGGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCCGGCGGAAAATGTTTGTGGTGTTATCCACCTGTTGGGCTTTGGTGGCGAGCGTATGGGTGTCGAGCTTCAGGCGCAGATAGCCGAGCgg > 2:98004/1‑251 (MQ=255) gtagtaATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGt < 1:266490/250‑1 (MQ=255) aTGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGtttttccc < 1:150506/251‑4 (MQ=255) tGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGGTTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGGGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAACCGAGCGGTCCGtttttcccg > 1:68577/1‑247 (MQ=255) tctcGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGTTCAGCACTACGCCGATTGCCATTGAAAGCAGCAACATCATGCGTAAAATGTTAGTGGTGTTATCCACTTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGTCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGAc < 1:172193/250‑1 (MQ=255) tctcGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGAGGTTCTCAGGAAGTTCCTTTGCTTCCAGCGGGTACGTTTACCCTGTAGCCGCGTGCGGGTCCGCACTACCCCGCTTGCCAGTGAAAGCAGCCACATACGGGGAAAAATGGTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGGGCGTCCGGCGCAGATAACCGAGCGGTCCGtttttcacggctattggttcgtc > 2:185861/1‑232 (MQ=255) ccGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATa < 1:59681/250‑1 (MQ=255) aGGAAGGGCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTCAAATAGCCGCCCGCTTTTTTACCGTCGAgcgc > 1:248956/1‑251 (MQ=255) ggCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCAGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCGGTGGGGCCACGGTGGCGAGCGTATGGGTGTCGAGCGGCAGGCGCAGATTGTCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGTCAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCg < 1:20263/251‑1 (MQ=255) gCGATTGCTGCCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAACAGCAAAATCAGGCGTAAAATGTTAGTGGTGTTATCCAACTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCAGCCCGCTTTTTTACCGTCGAGCGACAGCCgg > 1:16028/1‑251 (MQ=255) gagtgttgcCAGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTTCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGAAATTGGTTCGACAATCTGCTTGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCGGCCGGTc < 1:86111/245‑1 (MQ=255) aGCGGGTACGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACGCCGATTGCCAGTGAAAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGCGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTCCCGGCAATTGGTTCGACAATCTGCTGGTTAAAATAGCCGCCCGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCg > 1:209745/1‑251 (MQ=255) | CTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATGAGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTTAAAATAGCCGCCTGCTTTTTTACCGTCGAGCGCCAGCCGGTCGCGCACTTCG > NC_000913/4623985‑4624469 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |