Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,623,983 T→C V45V (GTA→GTG)  lplA ← lipoate‑‑protein ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,623,9830TC97.3% 108.4 / ‑4.2 37V45V (GTA→GTG) lplAlipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (12/24);  total (12/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.83e-01

TTCACCACCAGATCGTTACGTCCGGACGCTTCGGCGCTGACGCCGAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATG‑AGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTG  >  NC_000913/4623738‑4624226
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    
ttCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATcacta                                                                                                                                                                                                                                                  <  1:199488/250‑1 (MQ=255)
     caccaGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCgtgt                                                                                                                                                                                                                                            >  1:37029/1‑251 (MQ=255)
             cgtttcgccctgACGCTTCGGCCCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAAGCGGCGTTTAGATGGTTTTTTCGTACTCCGGCTTGCCTGCCATAAAGGTAAAGCATGTATTGCCGAGATCGTGGAACACCTCGCCGCCACCGCTACTGCTCCGTTCCTGGCGGACGTGATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCTCTAGCGTGTCTGCATTg                                                                                                                                                                                                                                    <  2:256006/240‑1 (MQ=255)
             cGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCAGTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGGCGGCAtt                                                                                                                                                                                                                                     <  2:237684/250‑1 (MQ=255)
                     acGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCCTTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCCTTGCGCCag                                                                                                                                                                                                                              >  2:1576/2‑249 (MQ=255)
                          cGCTTCGGCGCTGAGGCCAAGCGCTTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTATCCCGGCTGGCCAGCCGTAAAGGTAAAGCAGGTATTGCCGATATCGTGGAACACCGCTCCTCCACCGCTACTTCGCCGTGCCAGGCGGACGTGATCTTCTACCATCCTCCGGGTATTACACTCTTTCGATGTGTTCTGCGCGCTACCAAACACTACGGTGTCGGCATTTCGTCTGAGAAACa                                                                                                                                                                                                                       <  2:123214/251‑1 (MQ=255)
                                          ccaaGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATAGCCGAGATCGTGGAACACCGCGCCGCCACCGCTTCTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCAGCCGCCGGGTATTACACTCTTGCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGc                                                                                                                                                                                                       <  1:162035/248‑1 (MQ=255)
                                                      aGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCg                                                                                                                                                                                           <  1:195386/251‑1 (MQ=255)
                                                           aTTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACGCTTTCCACGGGTTCTGCGCTCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGaaaaa                                                                                                                                                                                      <  1:126916/251‑1 (MQ=255)
                                                            ttGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCGGGCGAAAAAt                                                                                                                                                                                     >  2:189357/1‑251 (MQ=255)
                                                              gAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATac                                                                                                                                                                                   <  1:193030/251‑1 (MQ=255)
                                                                       cGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAGTACACtcctccc                                                                                                                                                                          >  2:256462/1‑250 (MQ=255)
                                                                        gACGTGGAGATGGTTTTAGCGTACGCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTGTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGTCGAAAAATACTCTCCTCCAc                                                                                                                                                                         <  2:63248/251‑1 (MQ=255)
                                                                                    gTTTTATCGTACGCCTGCTGGCCAGCCAGAAAGGTAAAGCAGGTATGGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTaaa                                                                                                                                                             <  2:25027/251‑1 (MQ=255)
                                                                                              acgccGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCg                                                                                                                                                   <  1:256462/248‑1 (MQ=255)
                                                                                                cccGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTa                                                                                                                                                 <  1:223241/250‑1 (MQ=255)
                                                                                                 cccgCTTGCCAGCCATAAAGGTAAAGCAGGGATTGCCGAGATCGGGGAACACCGCGCCGCCCCCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCAGCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACGCCTCCTCCTCCAGGTTAAACCACGGGTCGt                                                                                                                                                  <  2:37029/246‑1 (MQ=255)
                                                                                                 ccGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGt                                                                                                                                                  <  1:33938/249‑1 (MQ=255)
                                                                                                   ggCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACCCCGCGCCGCCACCGCTACTGCGCCGTGACAGGCGGACGTTATCTTCTTCCATCCGCCGGGCATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCTCTACCGTGTCGGCATTGCGCCAGCGCAACCGATCGCGCTGCGTGCCGGGCATCTGGCGAAAAATACACTCTTCCACCGCAAGGTTATACCACgtggctta                                                                                                                                                 >  1:230059/1‑241 (MQ=255)
                                                                                                    gCTTGCCAGCCAGAAAGGTAAAACAGGTAGTGCCGAGATCGTGGAACCCCGCGCCGCCACCGCTACGGCGCCGTGCCAGGCGGACGTTATCTTCTTCCCTCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAagag                                                                                                                                             <  2:151641/251‑1 (MQ=255)
                                                                                                        gccagccaGAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTTCTGCTCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCa                                                                                                                                          <  2:65861/250‑1 (MQ=255)
                                                                                                                   aGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATG‑AGCAgg                                                                                                                             >  2:205816/1‑251 (MQ=255)
                                                                                                                     gTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCCCCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCATAGATG‑AGCAGGCg                                                                                                                           <  1:201172/251‑1 (MQ=255)
                                                                                                                                  tGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATG‑AGCAGGCGTAATGTGGACAt                                                                                                               >  1:77225/1‑250 (MQ=255)
                                                                                                                                   gCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATG‑AGCAGGCGTAATGTGGACATaa                                                                                                             <  1:130753/251‑1 (MQ=255)
                                                                                                                                                   cccGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATG‑AGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTgt                                                                                             <  1:240085/250‑1 (MQ=255)
                                                                                                                                                   ccagcgcCGCCACCGCTCCGGCGCCGTTCCAGGCGGACGTTAACTTCTGCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTGCCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCAGCTGGCGAAAAATACACTCCGCCACCGCTAGGTTAAACCACGGGTCGTAAGAGTCAGAGATG‑AGCATGCGTAATGTGGACATAACGATTTCCTTGCTTgt                                                                                             <  2:264601/248‑1 (MQ=255)
                                                                                                                                                              aCCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACCGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGACAGAGATGCACCAG‑CGTAATGTGGCCATAACGATTTCCTTTCTTGCAGTAGTGGGt                                                                                   >  2:8567/1‑250 (MQ=255)
                                                                                                                                                                 gCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGGGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGTTTAAACCACGGGTCGTAAGAGTCAGAGATG‑AGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCAct                                                                               <  1:237355/251‑1 (MQ=255)
                                                                                                                                                                  cTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATG‑AGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCActc                                                                              >  1:166925/1‑251 (MQ=255)
                                                                                                                                                                           cgtgccAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATG‑AGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTtctc                                                                     >  1:247931/4‑251 (MQ=255)
                                                                                                                                                                                 aGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATG‑AGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCttttaaaagagtgac                                                               <  1:89182/251‑12 (MQ=255)
                                                                                                                                                                                                tcttcCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATG‑AGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCa                                                 <  1:205816/250‑1 (MQ=255)
                                                                                                                                                                                                   tcCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATG‑AGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCAcc                                               <  1:149131/249‑1 (MQ=255)
                                                                                                                                                                                                         cgccgGGTATTACACTCTTTCAACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATG‑AGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCCCCGGTTTGCt                                       >  2:59681/1‑251 (MQ=255)
                                                                                                                                                                                                                    acacacTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCATTGCGCCAGAGCAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAATTACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATG‑AGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGCAATGGGTCACTCTTTTTTTTCGATTTCTTCCTCTTCCGGCACCGGGTGTCTGGCGGtttac                             >  2:150506/1‑247 (MQ=255)
                                                                                                                                                                                                                         ctTTCCACGGGTTCTGCGCGCGACCAATCACTGCCTTGTCGGCAGTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAATCCACGGGTCGTAAGAGTCAGAGATG‑AGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGTGTCACTCGTTTTACTCGCTGTCTTCCGCTTCCGGCACCGGTTTGCTGGCGTTTACCAGGa                         <  2:136126/249‑1 (MQ=255)
                                                                                                                                                                                                                                              gACCAATCACTTCCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATCTGGCGAAAAATACACTCCTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATG‑AGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTTGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGta  <  2:195214/251‑2 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    
TTCACCACCAGATCGTTACGTCCGGACGCTTCGGCGCTGACGCCGAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACAGAACGCGCTGCGTGGCGGGCATTTGGCGAAAAATACACTCTTCCACCGCCAGGTTAAACCACGGGTCGTAAGAGTCAGAGATG‑AGCAGGCGTAATGTGGACATAACGATTTCCTTTCTTGTAGTAATGGGTCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTG  >  NC_000913/4623738‑4624226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑