| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,623,782 | G→A | L112L (CTC→CTT) | lplA ← | lipoate‑‑protein ligase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,623,782 | 0 | G | A | 96.7% | 83.9 / ‑5.7 | 31 | L112L (CTC→CTT) | lplA | lipoate‑‑protein ligase A |
| Reads supporting (aligned to +/- strand): ref base G (1/0); major base A (12/17); minor base C (1/0); total (14/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01 | |||||||||||
GGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACGGTTTTCACCACCAGATCGTTACGTCCGGACGCTTCGGCGCTGACGCCGAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACA > NC_000913/4623540‑4624014 | ggCAGCAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTCAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGGCGCTTCGGCGCTGACGCCAAGCGCGtt > 1:94253/1‑251 (MQ=255) gcagcAGCTCGGTGAGGTTGGTCACGCGGGAACGCACTGAGGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCCGCCGCCACGCGCGTTc > 2:85257/5‑251 (MQ=255) cACGCGGGAACGCTCTGAGGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACg < 2:213178/251‑1 (MQ=255) actgaggTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACTTGGAGATGGtttt < 1:212130/245‑1 (MQ=255) agtGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGGGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTAc < 2:147838/248‑1 (MQ=255) tGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGCGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACGGTTTTCACCCCCAGATCGTTACGTCCGGCCGCTGCGGCGCTGCCGCCGCGCGCGTTCAGCGCCTTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTcc > 2:195386/1‑251 (MQ=255) tcccctccAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGGGGAAGCCCCGATCTTTGGTTTCGCGATAGGCCGAACCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCtt < 1:143132/244‑1 (MQ=255) cAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGAGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGAATTTGCGGTCGCCTTCGGCGGTTTTCACCACCAAATCGTTAAGCCCGGACGCTTCCGAGCTGACGACACGCGCGGTCAGCGCATTGAGCACCATCGACCGGGAGATTGTTTTGTCGTACTCCGGCGTgccagcc > 2:267869/1‑250 (MQ=255) ttttATCCGGATTGAGATAGTTTGCCAGGCGGCTAAGGTCGGCATTCAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGGACTCCGGCTTGCCAGCCATAAAGGt < 2:94253/250‑1 (MQ=255) gaTAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCg > 1:237684/1‑251 (MQ=255) tAGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGGGCATTGAGCACAATAGACGGGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCCGGGATTGcag > 1:184823/1‑247 (MQ=255) aGTTTGCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGGACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCgaga < 1:26949/251‑1 (MQ=255) tggCCAGGCGGCTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACGTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCATCCATAAAGGTAAAGCAGGTATTGCCGAGATc < 2:22055/248‑1 (MQ=255) gcggcTAAGGTCGGCATTGAGCAGCAAGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGGATTGCCGAGATCGTGGaaac > 1:25027/1‑249 (MQ=255) aGGTGCCGTGGTGGAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTgcgccgt < 1:85257/251‑2 (MQ=255) gtggAAGCCACGATCTTTGGTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCTTGCCAGGCGGa < 1:175573/251‑1 (MQ=255) tttGGTTTCGCGATAGGCCGATTCTGAGATGTTGCTGTCGCCTTCGGCGGTTTGCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTGAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCTCCGCTACTGCGCCGTTCCAGGCGTACGTTATCTTCTTcc < 2:170030/250‑1 (MQ=255) tggattCGCGATAGGCCGATCCTGAGACTTTGCGGTAGCCTTCGGCGGTTTTCAACACCAGATCGTTACGCCCGGACGCCTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCAAAATCGACGTGGGGGTGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGGATTGCCGGGGTCGTGGGACACCGCGCCGCCACCGCAACTGCGCCGTGCCAGGCGGGCGTTATCTTCTTCCATc > 2:193030/5‑251 (MQ=255) tcgTTTCGCGATAGGCCGTTCCTGATCCTTTGCGGTCGCCTTCGGCGGTTTTCCCCACCAGATCGGTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCTCAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCCCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCAt < 1:165983/248‑1 (MQ=255) ggTTTCGCGATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATcc < 1:155775/251‑1 (MQ=255) cgcgATAGGCCGATCCTGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAAACGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTAATGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCgg > 1:195214/1‑251 (MQ=255) cgatcctgactcTTTGCGGTCGCCATCGGCTGTCTTCACCACCAGGTCTGTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCTTATCATTGAGCACAGTCCGCGTGGAGATGGTTTTATCGTACTCCAGCGTGTCAGCCATAAAGGTATAGCAGGTATTGCGTAGTTCGTGGCACCCCGCGCCTCCACCGCTACTCCGCCGTTCCAGGCATACGTTATCTTCTTCCATCCGCCGGGTATTACAct < 1:117183/240‑1 (MQ=255) tGAGACTTTGCGGTCGCCTTCGGCGGTTTTCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCCAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGGCGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTcc > 2:33938/1‑251 (MQ=255) tcggcGGTTTTCACCACCCGATCGTTAAGCCCGGACGATTCGGCGCTGACGCCAAGCGCGTTCAGGGCATTGAGCACAATCGACGCGGAGATGGTTTTATCGTAATCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCCAGATCGTGGAACAACGCGCCGCCACCGCTACTGCGCCGTGCCAAGCGGGAGTTATCTTCTTACATCCCCCGGGGATTACCCTCCTTCCACGGGTTCTGCGCCCGAcc > 2:130753/5‑251 (MQ=255) ttCACCACCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATcacta < 1:199488/250‑1 (MQ=255) caccacCAGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTCCACTCTTTCCACGTGTTCTGCGCGCGAcccctcactaccg > 1:226647/1‑240 (MQ=255) caccaGATCGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCgtgt > 1:37029/1‑251 (MQ=255) cgtttcgccctgACGCTTCGGCCCTGACGCCAAGCGCGTTCAGCGCATTGAGCACAAGCGGCGTTTAGATGGTTTTTTCGTACTCCGGCTTGCCTGCCATAAAGGTAAAGCATGTATTGCCGAGATCGTGGAACACCTCGCCGCCACCGCTACTGCTCCGTTCCTGGCGGACGTGATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCTCTAGCGTGTCTGCATTg < 2:256006/240‑1 (MQ=255) cGTTACGCCCGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCAGTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGGCGGCAtt < 2:237684/250‑1 (MQ=255) acGGACGCTTCGGCGCTGACGCCAAGCGCGTTCAGCGCCTTGAGCACAATCGACGTGGAGATGGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGTGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATCACTACCGTGTCGGCCTTGCGCCag > 2:1576/2‑249 (MQ=255) cGCTTCGGCGCTGAGGCCAAGCGCTTTCAGCGCATTGAGCACAATCGACGTGGAGATGGTTTTATCGTATCCCGGCTGGCCAGCCGTAAAGGTAAAGCAGGTATTGCCGATATCGTGGAACACCGCTCCTCCACCGCTACTTCGCCGTGCCAGGCGGACGTGATCTTCTACCATCCTCCGGGTATTACACTCTTTCGATGTGTTCTGCGCGCTACCAAACACTACGGTGTCGGCATTTCGTCTGAGAAACa < 2:123214/251‑1 (MQ=255) | GGCAACAGCTCGGTGAGGTTGGTCACGCGGGAACGTACCGACGTAATGCCTTTCGCCGCCAGTTTCTTTTTATCCGGATTGAGATAGTTTGCCAGGCGGCTGAGGTCGGCATTGAGTAGCAAGGTGCCGTGGTGGAAGCCGCGATCTTTGGTTTCGCGATAGGCCGAGCCTGAGACTTTGCGGTCGCCTTCGACGGTTTTCACCACCAGATCGTTACGTCCGGACGCTTCGGCGCTGACGCCGAGCGCGTTCAGCGCATTGAGCACAATCGACGTGGAGATAGTTTTATCGTACTCCGGCTTGCCAGCCATAAAGGTAAAGCAGGTATTGCCGAGATCGTGGAACACCGCGCCGCCACCGCTACTGCGCCGCGCCAGGCGGACGTTATCTTCTTCCATCCGCCGGGTATTACACTCTTTCCACGGGTTCTGCGCGCGACCAATTACTACCGTGTCGGCATTGCGCCAGAGAAACA > NC_000913/4623540‑4624014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |