Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	969	T→G	A211A (GCT→GCG)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	969	0	T	G	100.0%	54.3 / NA	18	A211A (GCT→GCG)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (12/6);  total (12/6)

CAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCAC  >  NC_000913/765‑1207                                                                                                                                                                                                             |                                                                                                                                                                                                                                               caACGTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGAATTgg                                                                                                                                                                                                   >  1:9183/1‑250 (MQ=255)     gTTACCGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTACGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGCGATTTggacgg                                                                                                                                                                                               >  1:250914/1‑250 (MQ=255)                                                       cGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTACGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGCCGGCCGTTGACGGGGGCTATACCTGCGGCCCGCGTCAGGTGCCCGATGCGAGgt                                                                                                                                            >  1:20979/1‑251 (MQ=255)                                                              aCCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGTCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTT‑ACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGa                                                                                                                                      >  1:135321/1‑249 (MQ=255)                                                                                            aTTGCGGCAAGTCGCATTCCGGCTGATCACATGGAGCTGATGGCAGGTTTCACCGCCGGTAATGACAAAGGCGAACTGGTGGAACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGAGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGCCAGGTGCCCGGTGCGAGGTTGTTGTAGTCGATGTCCTACCAGGAAGCGATGGaact                                                                                                       >  1:117149/1‑248 (MQ=255)                                                                                                    atggcccatcccGGCGGATCACGTGGTGTGGATGGCAGTTGTCTCCGCCGGTAAAGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCGGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACt                                                                                               <  1:154458/241‑1 (MQ=255)                                                                                                    acgtcGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGCGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGGTGTTGAAGTCGATGTCCTACCCGGAAGCGAt                                                                                                             >  2:1300/5‑237 (MQ=255)                                                                                                    aagtcGCATTCCGGCTGATCACATGGTGCTGATGGCAGTTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGAt                                                                                                             <  1:1300/233‑1 (MQ=255)                                                                                                                    gATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTACTTGGCCGCAACGGTTCCGACTCCTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGCTTTGGACGGACGTTGACGGGGTCTATACCTGCGGCCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGCAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGttct                                                                               >  1:257686/1‑251 (MQ=255)                                                                                                                         cctggagCTGATGGCAGGTTTCCCCGCCGGTAATGAAAAAGGAGAACTGGTGGTACTTGGACGCACCGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTTCGCGCCGCTTTTTGCGAGATTTTGACGGACGTTGACGGGGTCTTTACCTGCGACCCGCGTCAGGGGCCCCATGCGAGGTTGTTGAAGTCGATGTCCTACCCGGAAGCGATGGAGCTTTCCTACTTCGGCGCGAAAGttctttccc                                                                          >  2:186235/7‑247 (MQ=255)                                                                                                                                        gtgttccACCGCCGGTAATGAAAAAGGCTAACTGGTGGTTCTTGGTCGCAACGGTTCCGTCTACTCCGCGGCGGTGCTGGCTTCCTGTTTACGCGCCGATTGTTGCGAAATGTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCTATTCTAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTACCTACTTCGGCGCTAAAGTCCTGCACCCCCTCGCCAGTAccc                                                           <  2:117149/245‑1 (MQ=255)                                                                                                                                                         aTGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCg                                                                                                               >  1:205063/1‑182 (MQ=255)                                                                                                                                                         aTGAAAAAGGCGAACTGGTGGTACTTGGACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCg                                                                                                               <  2:205063/182‑1 (MQ=255)                                                                                                                                                                                   ggACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCGGCCTGTTTACGCGCCGATTGTTGCGCGATTTGGACGGACGTTGCCGGGGTCTATACCTGCGACCCGCGTCCGGTGGCCGATGCGAGGGTGTTGAAGGTGATGTCCCACCCGGAAGCGATGGGGCTTTCCTCCTTCGGCGCTACAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCgg                >  2:125756/1‑251 (MQ=255)                                                                                                                                                                                    gACGCAACGGTTCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGa               <  1:225840/251‑1 (MQ=255)                                                                                                                                                                                    gACGCAACGGTTCCGACTACTACGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCACCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGa               <  1:3377/251‑1 (MQ=255)                                                                                                                                                                                               tCCGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATAACTGCGAACCGCGTAAGGTGCCCGATGCGAGGGTGTTGAAGGCGATGTCCTACCAGGCAGCGATGGAGCTTTCCGACTTCGGGGCTAAAGTTCTTCACCCCCGCACCATTACCACCATCGCCCAGTTCCAGATCCCTTGCCCGATTAAAAATACCGGaaaacattcagc    >  1:107179/1‑242 (MQ=255)                                                                                                                                                                                                 cGACTACTCCGCGGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGctc  >  1:143534/1‑249 (MQ=255)                                                                                                                                                                                                             |                                                                                                                                                                                                                                               CAACGTTACTGTTATCGATCCGGTCGAAAAACTGCTGGCAGTGGGGCATTACCTCGAATCTACCGTCGATATTGCTGAGTCCACCCGCCGTATTGCGGCAAGCCGCATTCCGGCTGATCACATGGTGCTGATGGCAGGTTTCACCGCCGGTAATGAAAAAGGCGAACTGGTGGTGCTTGGACGCAACGGTTCCGACTACTCTGCTGCGGTGCTGGCTGCCTGTTTACGCGCCGATTGTTGCGAGATTTGGACGGACGTTGACGGGGTCTATACCTGCGACCCGCGTCAGGTGCCCGATGCGAGGTTGTTGAAGTCGATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGCAC  >  NC_000913/765‑1207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑