Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,623,336	G→C	T261S (ACC→AGC)	lplA ←	lipoate‑‑protein ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,623,336	0	G	C	100.0%	93.5 / NA	29	T261S (ACC→AGC)	lplA	lipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (18/11);  total (18/11)

CGGGTAACTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCG  >  NC_000913/4623094‑4623568                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                         cagcgagttACCTTACCGCCCCCGCTATCCACGTCGACAACTTCCGTAGCTCTTTTTCCTGGTCCGGGAAGTCAACCAACAGCGCTTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGGCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGt                                                                                                                                                                                                                                  >  1:27894/9‑251 (MQ=255)  agcgagttACCTTACCGCCCCCGCTATCCACGTCGACAACTTCCGTAGCTCTTTTTCCTGGTCCGGGAAGTCAACCAACAGCGCTTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGtt                                                                                                                                                                                                                                 >  1:169635/8‑251 (MQ=255)                                  ccGACAACTTCCGTAGCTCTTTTTCCTGGTCCGGGAAGGCACCCAACAGCGCTTCTCCCTCGTGTTGCAGCATATCCGCGCTGTACAGGCCGCCTTGCATTCGCCCGTCGAGGGCTTCCAGCGGCGCGGGGTTGAGTCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGGTCATCCAGCTGATGCGAGAATGCCGGATCCTGa                                                                                                                                                                                                 <  2:240572/251‑1 (MQ=255)                                    gacaaCTTCCGTAGCTCTTGTTCCTGGTCCGGGAAGTCAACCAACAGCGCCTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGAcc                                                                                                                                                                                               <  1:160955/248‑1 (MQ=255)                                                          ccTGGTCCGGGAAGTCAACCAACAGCGCTTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTActc                                                                                                                                                                         >  1:34225/1‑251 (MQ=255)                                                               tCCGGGAAGTCAACCAACAGCGCTTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGCTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTgacg                                                                                                                                                                    >  1:189745/1‑248 (MQ=255)                                                                   ggAAGTCAACCAACAGCGCTTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGGTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTGCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCTCATCTACTCTGACGGGCa                                                                                                                                                                <  2:27894/251‑1 (MQ=255)                                                                      aGTCAACAAACCGCGCTTCGCACTCTTGTTGCAGCGTATCCGCGAGGTACAGGCAGCCTTGCAATCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCATTTTTCAACGTTGAAATGCAGTTCCACGCCGCCCCAGCTACAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCATGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCaa                                                                                                                                                               >  2:26949/1‑249 (MQ=255)                                                                          aaccaacAGCGCTTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACGCTGACGGGCAAAGGttt                                                                                                                                                         <  1:74337/251‑1 (MQ=255)                                                                           accaacAGCGCTTCGCGCTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGGCGCCCGGCGAGGGCTTCCCGCGGCGCGGGGTTGAGGCTGGCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGGTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGGTCCATGCCCAGCTACTCTGACGGGCAAAGGTTTc                                                                                                                                                        <  2:2450/251‑1 (MQ=255)                                                                           accaacAGCGCTTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATGCCCAGCTACTCTGACGGGCAAAGGTTTc                                                                                                                                                        <  2:2439/251‑1 (MQ=255)                                                                                 aGCGCTTCGCACTCTTGTTGCAGCATACCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCGGACGGGCAAAGGTTTCGGCGaa                                                                                                                                                  <  2:34225/251‑1 (MQ=255)                                                                                                 gTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGc                                                                                                                                  >  2:45135/1‑251 (MQ=255)                                                                                                          tatCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGATGAGGCTGTCGGTAAACACCTGGGCGCGGGAGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGCCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGtcc                                                                                                                         >  2:143132/1‑249 (MQ=255)                                                                                                           atccgcgcGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGtccg                                                                                                                        >  2:74180/5‑248 (MQ=255)                                                                                                                     tacagaccgacTTGCAGTCGCACGGCGCGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACCCCTGGGCGCGGGCGATATGGCCTTTTTCAACGTCGAAATGCAGATCCACGCCGCCCCAGCTAAAGCGATCATCCAGCAGCAGCGAGAATGCCGGCGCCTGACCGAAGCTCCATTCCCCGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAgatgat                                                                                                              >  2:238526/11‑251 (MQ=255)                                                                                                                      aCAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAgatgat                                                                                                              <  1:74180/250‑1 (MQ=255)                                                                                                                                               gAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAAt                                                                                    >  1:189421/1‑251 (MQ=255)                                                                                                                                                         aGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGaaaa                                                                           >  1:147838/1‑250 (MQ=255)                                                                                                                                                            ggcgCGGTGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTACAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGc                                                                       <  1:68017/251‑1 (MQ=255)                                                                                                                                                                      tGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCACCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGACTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATg                                                             >  1:170030/1‑251 (MQ=255)                                                                                                                                                                                           gggCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATgg                                        <  1:45135/251‑1 (MQ=255)                                                                                                                                                                                              cgcgGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGAGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTCCTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTg                                     >  1:22055/1‑251 (MQ=255)                                                                                                                                                                                                      atatGGCCTTTTTCAACGTAGACATGCAGTTCCACGCCGCCCCAGCTCAAGCGATCATCCAGCAGATGCGAGACTGACGGCGCCTGACCGAAGTTCCCTTCCCTGCTACTCTGACGGGCAACGGCTTCGGCGCAGTTTGGCAAGTCTGGCGTTTTGTGCGGGGAGATGATTTCCGCTTCTACACCCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGTTCCCCgg                             >  2:267148/1‑251 (MQ=255)                                                                                                                                                                                                                  tCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCCTCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTAATCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAACGGACTTGGTTATGGCCTCGCAAACCTGCTCCTGGGTGATCCCCGGCAGCAGCTCggg                 >  1:123214/1‑250 (MQ=255)                                                                                                                                                                                                                      cGTCGAAATGCAGTTCCACGCCGACCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGATACTCTGCCGGGCAAAGGTTTCGCCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGCAAACGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGAAGCTCGGGGAgg             >  2:240085/1‑251 (MQ=255)                                                                                                                                                                                                                       gTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGGGATCCCCGGCAGCAGCTCGGTGAGGt            >  1:256006/1‑251 (MQ=255)                                                                                                                                                                                                                           aaaTGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAAGGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGGt        >  1:86470/1‑251 (MQ=255)                                                                                                                                                                                                                                 aGTTCCAAGCCGCCCCAGCTAAAGCGATCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATCCCCAGCGACTCTCACGGGCAAAGGTTTCGGCGAAGTGTGGCAAGTCTGGCGTTTTGTCCGGGGAGATGATTTCCGCTTCTACACGCTCGCCATAATGGGCGAAAAATGCCTTGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAGCAGCTCGGTGAGGTTGTTCAcgcg  <  1:267148/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                         CGGGTAACTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCATAATGGGCGAAAAAGGCCTCGGTTATGGCCTCGCAAACCTGCTCATGGGTGATCCCCGGCAACAGCTCGGTGAGGTTGGTCACGCG  >  NC_000913/4623094‑4623568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑