Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,623,230	T→C	G296G (GGA→GGG)	lplA ←	lipoate‑‑protein ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,623,230	0	T	C	100.0%	48.6 / NA	19	G296G (GGA→GGG)	lplA	lipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/9);  total (10/9)

CGGGTAACTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGAT  >  NC_000913/4623094‑4623460                                                                                                                                         |                                                                                                                                                                                                                                       cagcgagttACCTTACCGCCCCCGCTATCCACGTCGACAACTTCCGTAGCTCTTTTTCCTGGTCCGGGAAGTCAACCAACAGCGCTTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGGCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGt                                                                                                                      >  1:27894/9‑251 (MQ=255)  agcgagttACCTTACCGCCCCCGCTATCCACGTCGACAACTTCCGTAGCTCTTTTTCCTGGTCCGGGAAGTCAACCAACAGCGCTTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGtt                                                                                                                     >  1:169635/8‑251 (MQ=255)                                  ccGACAACTTCCGTAGCTCTTTTTCCTGGTCCGGGAAGGCACCCAACAGCGCTTCTCCCTCGTGTTGCAGCATATCCGCGCTGTACAGGCCGCCTTGCATTCGCCCGTCGAGGGCTTCCAGCGGCGCGGGGTTGAGTCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGGTCATCCAGCTGATGCGAGAATGCCGGATCCTGa                                                                                     <  2:240572/251‑1 (MQ=255)                                    gacaaCTTCCGTAGCTCTTGTTCCTGGTCCGGGAAGTCAACCAACAGCGCCTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGAcc                                                                                   <  1:160955/248‑1 (MQ=255)                                                          ccTGGTCCGGGAAGTCAACCAACAGCGCTTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTActc                                                             >  1:34225/1‑251 (MQ=255)                                                              gtCCGGGAAGTCAACCAACAGCGCTTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCaa                                                                                                                                                            <  2:1306/151‑1 (MQ=255)                                                              gtCCGGGAAGTCAACCAACAGCGCTTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCaa                                                                                                                                                            >  1:1306/2‑152 (MQ=255)                                                               tCCGGGAAGTCAACCAACAGCGCTTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGCTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTgacg                                                        >  1:189745/1‑248 (MQ=255)                                                                   ggAAGTCAACCAACAGCGCTTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGGTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTGCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCTCATCTACTCTGACGGGCa                                                    <  2:27894/251‑1 (MQ=255)                                                                      aGTCAACAAACCGCGCTTCGCACTCTTGTTGCAGCGTATCCGCGAGGTACAGGCAGCCTTGCAATCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCATTTTTCAACGTTGAAATGCAGTTCCACGCCGCCCCAGCTACAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCATGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCaa                                                   >  2:26949/1‑249 (MQ=255)                                                                          aaccaacAGCGCTTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACGCTGACGGGCAAAGGttt                                             <  1:74337/251‑1 (MQ=255)                                                                           accaacAGCGCTTCGCGCTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGGCGCCCGGCGAGGGCTTCCCGCGGCGCGGGGTTGAGGCTGGCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGGTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGGTCCATGCCCAGCTACTCTGACGGGCAAAGGTTTc                                            <  2:2450/251‑1 (MQ=255)                                                                           accaacAGCGCTTCGCACTCTTGTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATGCCCAGCTACTCTGACGGGCAAAGGTTTc                                            <  2:2439/251‑1 (MQ=255)                                                                                 aGCGCTTCGCACTCTTGTTGCAGCATACCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCGGACGGGCAAAGGTTTCGGCGaa                                      <  2:34225/251‑1 (MQ=255)                                                                                                 gTTGCAGCATATCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGc                      >  2:45135/1‑251 (MQ=255)                                                                                                          tatCCGCGCGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGATGAGGCTGTCGGTAAACACCTGGGCGCGGGAGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGCCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGtcc             >  2:143132/1‑249 (MQ=255)                                                                                                           atccgcgcGGTACAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGtccg            >  2:74180/5‑248 (MQ=255)                                                                                                                     tacagaccgacTTGCAGTCGCACGGCGCGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACCCCTGGGCGCGGGCGATATGGCCTTTTTCAACGTCGAAATGCAGATCCACGCCGCCCCAGCTAAAGCGATCATCCAGCAGCAGCGAGAATGCCGGCGCCTGACCGAAGCTCCATTCCCCGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAgatgat  >  2:238526/11‑251 (MQ=255)                                                                                                                      aCAGGCAGCCTTGCAGTCGCCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGCTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGACGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTCCGGGGAgatgat  <  1:74180/250‑1 (MQ=255)                                                                                                                                         |                                                                                                                                                                                                                                       CGGGTAACTACCTTACAGCCCCCGCCATCCATGCCGATAACTCCCGTAGCTCTTTTTCCTGTTCCGGGAAGTCAACCAACAGCGCTTCGCACTCCTGTTGCAGCATATCTGCGCGGTACAGGCAGCCTTGCAGTCGTCCGGCGAGGGCTTCCAGCGGCGCGGGGTTGAGGCTGTCGGTAAACACCTGGGCGCGGGTGATATGGCCTTTTTCAACGTCGAAATGCAGTTCCACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGAT  >  NC_000913/4623094‑4623460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑