Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,652	C→T	C350C (TGC→TGT)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,652	0	C	T	97.9%	134.7 / NA	49	C350C (TGC→TGT)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (28/19);  total (29/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAAAACAATGGCTAC  >  NC_000913/4620403‑4620890                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               cGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGACGCGGGTGATAACACCATCGTCTTCACCACCTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGCGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTATCTGCTGCGCGATGACGACATCCTGCTCCTTACTGCTGACCCCGGTTGCg                                                                                                                                                                                                                                               >  2:97809/1‑251 (MQ=255)   gTATCACCCAGAAAGTGCAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGCGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGAt                                                                                                                                                                                                                                             >  2:138504/1‑251 (MQ=255)     aTCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACAATCAAAGAGATGAAAGAAGCGGGTGATCACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGACGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGCTCCTCACTGCTGACCACGGTTGTGATcc                                                                                                                                                                                                                                           >  1:247041/1‑251 (MQ=255)                         aaCTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGAcc                                                                                                                                                                                                                       >  2:70783/2‑251 (MQ=255)                         aaCTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATAGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGAcc                                                                                                                                                                                                                       >  1:234783/2‑251 (MQ=255)                          aCTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGAcc                                                                                                                                                                                                                       >  1:101656/1‑250 (MQ=255)                               ccTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACAcgc                                                                                                                                                                                                                 >  2:261859/1‑251 (MQ=255)                                               gACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCCCCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGt                                                                                                                                                                                                 <  1:203930/251‑1 (MQ=255)                                                aCGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCggc                                                                                                                                                                                                 >  2:85783/1‑249 (MQ=255)                                                   ccaccaTCAAAGAGATGAAAGAAGCGGGTGATAACTCCATCGTCTTCCCCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGCCCACACGCGTTAACACATTCCGGTACTg                                                                                                                                                                                             <  2:226982/251‑1 (MQ=255)                                                     accaTCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGt                                                                                                                                                                                           >  1:168883/1‑251 (MQ=255)                                                          cAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGtttacg                                                                                                                                                                                      >  1:226929/1‑246 (MQ=255)                                                                     aagaagCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGCCCTGGACCGGTACTGACCACACGCGTGACCACATTCCGGTACTGGTTTCCGGCCCGAAAGt                                                                                                                                                                            >  2:162745/1‑250 (MQ=255)                                                                       gaagCGGGTGATACCACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTaaa                                                                                                                                                                         <  1:228141/251‑1 (MQ=255)                                                                        aagCGGGTGATAACACCATCGTCTTCACCAACTTCGGTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGAACGGTACTGAACACACGCGTGAACACATTCCGGTACTGGTTGACGGCCCGAAAGTaaaa                                                                                                                                                                        <  2:181378/251‑1 (MQ=255)                                                                                gATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCGTCCTGGGGCCACCGTCGCGACGTCGCCGGGTATGCTGCGGGTCTGGTGCTGTTCGTCCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACTACATCCTGATCCTCACTGCTGTCCACGGTTGGGATCCGACCTGTACCGGTACTGACCACACGCGTGAACCCATTCCGGTACTTGTTTACGGCCCGAAAGTAAAACCGGGCt                                                                                                                                                                 <  1:19966/250‑1 (MQ=255)                                                                                   aaCACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACt                                                                                                                                                             <  1:199780/251‑1 (MQ=255)                                                                                   aaCACAATCGTCTTCACCAACTCAGTTGACTTCGCCTCTTCCTGGCGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGATCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACt                                                                                                                                                             >  2:182343/1‑251 (MQ=255)                                                                                        cATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGGTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCACGAAAGGAAAACCGGGCTCACTGGGTc                                                                                                                                                        >  2:231361/1‑251 (MQ=255)                                                                                                 cACCAACTTCGCTGACGTCGACTCTTCCTGGGGCCACCGACGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGaa                                                                                                                                                <  1:206977/250‑1 (MQ=255)                                                                                                 cACCAACTTAGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAAACGGGCTCACTGGGTCACCGTGaa                                                                                                                                                >  1:89039/1‑250 (MQ=255)                                                                                                   ccAACTTCGTTGACTTCGACGCGTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGaaa                                                                                                                                               <  2:20637/249‑1 (MQ=255)                                                                                                    cacccgcGTTGACTTCCCCTCTTCCTGGGGACACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTTGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCGGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAGCCGGGCTCACTGGGTctccgagaaaccc                                                                                                                                            <  2:88532/245‑13 (MQ=255)                                                                                                      acctCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGGCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTGACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTc                                                                                                                                          <  1:138504/248‑1 (MQ=255)                                                                                                      aCTTCGTTGAAGTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTc                                                                                                                                          <  2:94385/251‑1 (MQ=255)                                                                                                                ccgcgacTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGGTATGCTGCGGGTCTGGAGCTGATCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATGCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGAGGCACCGGGAAACCTGCTCGggtgatg                                                                                                                                >  2:246063‑M2/4‑244 (MQ=255)                                                                                                                     aCTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCAGCGGGTCTGGAGCGGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTACGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGACACCTTCGCGggtgatgctgcc                                                                                                                           <  2:59558‑M2/251‑13 (MQ=255)                                                                                                                     aCTCTTCCTGGGGCCACAGTCGCGACGTCGCCGGCTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCAGGTACTGGTTTACGGCCCGAAAGTAAAAACGGGCTCACTGGGTCACCGTGAAACCTCCGCGggtgatgaggcc                                                                                                                           <  2:185004‑M2/251‑13 (MQ=255)                                                                                                                           ccTGGGGCCCCCGTCGCGACGTCGCCGGTTATTCTGCGGGTCTGGAGCTGTTCGACCGCAGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCCCGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGGTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgcaaactt                                                                                                                      >  2:180835‑M2/1‑233 (MQ=255)                                                                                                                           ccTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaactt                                                                                                                      >  1:261859‑M2/1‑233 (MQ=255)                                                                                                                                  ccACCGTCGCGACGTCGCCGGTTATGCTGCGGGGCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgattt                                                                                                              >  2:194916‑M2/1‑226 (MQ=255)                                                                                                                                                   ccGGATATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgttt                                                                                             <  1:244858‑M2/251‑43 (MQ=255)                                                                                                                                                    cGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttt                                                                                            <  1:115088‑M2/251‑44 (MQ=255)                                                                                                                                                      gTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttacggatttagtgtatgatggtgtttttg                                                                                          >  2:251146‑M2/1‑206 (MQ=255)                                                                                                                                                         aTGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgagg                                                                                       >  1:5093‑M2/1‑203 (MQ=255)                                                                                                                                                           gctgcgggtCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTAATGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAAAACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtg                                                                                     <  1:255320‑M2/248‑51 (MQ=255)                                                                                                                                                              gcgGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgct                                                                                   >  1:122444‑M2/1‑198 (MQ=255)                                                                                                                                                                           tGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggagatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgt                                                                     <  1:266329‑M2/251‑67 (MQ=255)                                                                                                                                                                               cGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGACAGTAAAACCGGGCTCACTGGGTCACCGTGAACCCTTCGCGggtgatgctgccaacttgctgatttcgtgtatgatggtgtttttgaggtgctcccgtggcttctgtttct                                                                 <  1:64533‑M2/251‑71 (MQ=255)                                                                                                                                                                                        tCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgt                                                        <  1:4862‑M2/251‑80 (MQ=255)                                                                                                                                                                                                       gTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagc                                          >  1:268417‑M2/1‑157 (MQ=255)                                                                                                                                                                                                                gcgcGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggg                                >  1:81605‑M2/1‑148 (MQ=255)                                                                                                                                                                                                                       gacgacATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACCCGCGTGAACACCTTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggagatgctgccaacttactgatttagtgtatgatggtgtttttgag                                                                                        <  2:37648‑M2/188‑48 (MQ=255)                                                                                                                                                                                                                       gacgacATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACGGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgag                                                                                        >  1:37648‑M2/1‑141 (MQ=255)                                                                                                                                                                                                                        acgacAGCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctccggttcagctactgacggggtggtgcg                        >  1:97809‑M2/1‑140 (MQ=255)                                                                                                                                                                                                                         cgacATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgt                       >  1:250661‑M2/1‑139 (MQ=255)                                                                                                                                                                                                                                            gCTGACCACGGTTGTGATCCGGCCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcgtctgtttctatcagctgtccctcctgttcagctactgacggggtggtgcgtaacggcaaaagcaccgcc     >  1:162745‑M2/1‑120 (MQ=255)                                                                                                                                                                                                                                            gCTGACCACGGTTGTGAGCCGACCTGGACCGGTACTGACCACACGCGTGAACACATCCCGGTACGGGTTTACGGCCCGAAAGAAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgaggctgccaacttactgatttattgtatgatggtttttttgaggtgctccagtggcttttgtttcaattagctgtccctcctgttcagctactgacgggggggtgcgtaacggcaaaagcaccgccg    >  2:125134‑M2/1‑120 (MQ=255)                                                                                                                                                                                                                                               gACCACGGTTGTGATCCGACCTGGACCGGTCCTGACCACACGCGTGAACACATTCCGGTACTGGTTAACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTTAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgctccagtggcttctgtttctatcagctgtccctccggttcagctactgacggggtggtgcgtaacggcaaaagcaccgccgga  >  2:226929‑M2/1‑117 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               CGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATGGCAAAGCCATGTTCTGATGGATTTGGGCGGAGCGTTGACTCCGCCTTTGTTATGTCACAAAAAGGATAAAACAATGGCTAC  >  NC_000913/4620403‑4620890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑