Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,571	A→G	E323E (GAA→GAG)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,571	0	A	G	100.0%	131.1 / NA	45	E323E (GAA→GAG)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/20);  total (25/20)

GAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATG  >  NC_000913/4620334‑4620809                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               gAAACGGGTTGATGAAAAACACGGTCAGGTGGGTTCTGTCGGTAAAAGTGCAGACATCGACGCCATCTGCGGTATCACCAAGAAATTGAAAGCAACTGGCCTGTACGCGCTGTTTGCCGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGAccgcc                                                                                                                                                                                                                                   <  2:220602/251‑1 (MQ=255)    acgggtggataaaaaaCACGGTCAGGTGTTTTCGGTCGGTAAAATTGCAGACATCTACGCCAACGGCGGTACCACCAAGAAAGGAAAAGCAACGGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGCCTTCACCAACTGCGTTGACTGCGACTCTTCCGGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTc                                                                                                                                                                                                                                <  2:48766/240‑1 (MQ=255)       ggTTGATGAAAAACACGGTCAGGTGGTTTGGGTCGGTAAAATTTCATACATCTACATCAACTGCGGTATCCCCAAGAAAGTCAAAGCATCTGGCCTGTACGCGCTGTTTGACGCCGCCACCAAATAGATGAAAGAAGCGGGTTATAACACCATCGTCTTCACCAACTTCGTTGACTGCGACGCTTCCTGGGGCCACCTTCGCGTCGTTGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCt                                                                                                                                                                                                                               <  1:164007/249‑1 (MQ=255)            atgaAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAg                                                                                                                                                                                                                        <  1:42100/251‑1 (MQ=255)                  aaCACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATg                                                                                                                                                                                                                  >  1:94385/1‑251 (MQ=255)                        gtcAGGTGGTTTCTGTCGGTAAAATTGCAGACGTCTACGCCCACGGCGGTATCACCACGAAAGTGAAAGCAACTGGCCTGGCCGCGCTGTTTGACGCCACCATCAAAGAGCTGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGCCGCCGGTTATGCTGCGGGTCTGGGGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTctg                                                                                                                                                                                                            >  2:268417/3‑251 (MQ=255)                              tggtTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTgcgc                                                                                                                                                                                                      >  2:122444/1‑251 (MQ=255)                                             aaaTTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAACGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTTTGCTGCGCGATGCCGACATCCTg                                                                                                                                                                                       >  2:169248/1‑251 (MQ=255)                                                                      cGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGACGCGGGTGATAACACCATCGTCTTCACCACCTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGCGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTATCTGCTGCGCGATGACGACATCCTGCTCCTTACTGCTGACCCCGGTTGCg                                                                                                                                                              >  2:97809/1‑251 (MQ=255)                                                                        gTATCACCCAGAAAGTGCAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGCGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGAt                                                                                                                                                            >  2:138504/1‑251 (MQ=255)                                                                          aTCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACAATCAAAGAGATGAAAGAAGCGGGTGATCACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGACGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGCTCCTCACTGCTGACCACGGTTGTGATcc                                                                                                                                                          >  1:247041/1‑251 (MQ=255)                                                                                              aaCTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGAcc                                                                                                                                      >  2:70783/2‑251 (MQ=255)                                                                                              aaCTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATAGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGAcc                                                                                                                                      >  1:234783/2‑251 (MQ=255)                                                                                               aCTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGAcc                                                                                                                                      >  1:101656/1‑250 (MQ=255)                                                                                                    ccTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACAcgc                                                                                                                                >  2:261859/1‑251 (MQ=255)                                                                                                                    gACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCCCCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGt                                                                                                                <  1:203930/251‑1 (MQ=255)                                                                                                                     aCGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCggc                                                                                                                >  2:85783/1‑249 (MQ=255)                                                                                                                        ccaccaTCAAAGAGATGAAAGAAGCGGGTGATAACTCCATCGTCTTCCCCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGCCCACACGCGTTAACACATTCCGGTACTg                                                                                                            <  2:226982/251‑1 (MQ=255)                                                                                                                          accaTCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGt                                                                                                          >  1:168883/1‑251 (MQ=255)                                                                                                                               cAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGtttacg                                                                                                     >  1:226929/1‑246 (MQ=255)                                                                                                                                          aagaagCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGCCCTGGACCGGTACTGACCACACGCGTGACCACATTCCGGTACTGGTTTCCGGCCCGAAAGt                                                                                           >  2:162745/1‑250 (MQ=255)                                                                                                                                            gaagCGGGTGATACCACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTaaa                                                                                        <  1:228141/251‑1 (MQ=255)                                                                                                                                             aagCGGGTGATAACACCATCGTCTTCACCAACTTCGGTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGAACGGTACTGAACACACGCGTGAACACATTCCGGTACTGGTTGACGGCCCGAAAGTaaaa                                                                                       <  2:181378/251‑1 (MQ=255)                                                                                                                                                     gATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCGTCCTGGGGCCACCGTCGCGACGTCGCCGGGTATGCTGCGGGTCTGGTGCTGTTCGTCCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACTACATCCTGATCCTCACTGCTGTCCACGGTTGGGATCCGACCTGTACCGGTACTGACCACACGCGTGAACCCATTCCGGTACTTGTTTACGGCCCGAAAGTAAAACCGGGCt                                                                                <  1:19966/250‑1 (MQ=255)                                                                                                                                                        aaCACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACt                                                                            <  1:199780/251‑1 (MQ=255)                                                                                                                                                        aaCACAATCGTCTTCACCAACTCAGTTGACTTCGCCTCTTCCTGGCGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGATCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACt                                                                            >  2:182343/1‑251 (MQ=255)                                                                                                                                                             cATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGGTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCACGAAAGGAAAACCGGGCTCACTGGGTc                                                                       >  2:231361/1‑251 (MQ=255)                                                                                                                                                                      cACCAACTTCGCTGACGTCGACTCTTCCTGGGGCCACCGACGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGaa                                                               <  1:206977/250‑1 (MQ=255)                                                                                                                                                                      cACCAACTTAGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAAACGGGCTCACTGGGTCACCGTGaa                                                               >  1:89039/1‑250 (MQ=255)                                                                                                                                                                        ccAACTTCGTTGACTTCGACGCGTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGaaa                                                              <  2:20637/249‑1 (MQ=255)                                                                                                                                                                         cacccgcGTTGACTTCCCCTCTTCCTGGGGACACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTTGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCGGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAGCCGGGCTCACTGGGTctccgagaaaccc                                                           <  2:88532/245‑13 (MQ=255)                                                                                                                                                                           acctCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGGCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTGACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTc                                                         <  1:138504/248‑1 (MQ=255)                                                                                                                                                                           aCTTCGTTGAAGTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTc                                                         <  2:94385/251‑1 (MQ=255)                                                                                                                                                                                     ccgcgacTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGGTATGCTGCGGGTCTGGAGCTGATCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATGCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGAGGCACCGGGAAACCTGCTCGggtgatg                                               >  2:246063‑M2/4‑244 (MQ=255)                                                                                                                                                                                          aCTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCAGCGGGTCTGGAGCGGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTACGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGACACCTTCGCGggtgatgctgcc                                          <  2:59558‑M2/251‑13 (MQ=255)                                                                                                                                                                                          aCTCTTCCTGGGGCCACAGTCGCGACGTCGCCGGCTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCAGGTACTGGTTTACGGCCCGAAAGTAAAAACGGGCTCACTGGGTCACCGTGAAACCTCCGCGggtgatgaggcc                                          <  2:185004‑M2/251‑13 (MQ=255)                                                                                                                                                                                                ccTGGGGCCCCCGTCGCGACGTCGCCGGTTATTCTGCGGGTCTGGAGCTGTTCGACCGCAGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCCCGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGGTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgcaaactt                                     >  2:180835‑M2/1‑233 (MQ=255)                                                                                                                                                                                                ccTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaactt                                     >  1:261859‑M2/1‑233 (MQ=255)                                                                                                                                                                                                       ccACCGTCGCGACGTCGCCGGTTATGCTGCGGGGCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgattt                             >  2:194916‑M2/1‑226 (MQ=255)                                                                                                                                                                                                                        ccGGATATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgttt            <  1:244858‑M2/251‑43 (MQ=255)                                                                                                                                                                                                                         cGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttt           <  1:115088‑M2/251‑44 (MQ=255)                                                                                                                                                                                                                           gTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttacggatttagtgtatgatggtgtttttg         >  2:251146‑M2/1‑206 (MQ=255)                                                                                                                                                                                                                              aTGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgagg      >  1:5093‑M2/1‑203 (MQ=255)                                                                                                                                                                                                                                gctgcgggtCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTAATGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAAAACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtg    <  1:255320‑M2/248‑51 (MQ=255)                                                                                                                                                                                                                                   gcgGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACTGCTGACCACGGTTGTGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGCTCACTGGGTCACCGTGAAACCTTCGCGggtgatgctgccaacttactgatttagtgtatgatggtgtttttgaggtgct  >  1:122444‑M2/1‑198 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               GAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTGATCCTCACCGCTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGATATGGAATATG  >  NC_000913/4620334‑4620809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑