Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,385	G→A	A261A (GCG→GCA)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,385	0	G	A	100.0%	129.6 / NA	42	A261A (GCG→GCA)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/20);  total (22/20)

CCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTG  >  NC_000913/4620138‑4620628                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    cctgccacgacggaacctttgcccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACCACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGAACAAGCCGGTAACTTCCAGCGTACCGGTAACCGCCACGCCCTGGCTGTTGAGCCGCCATCACCGCCCGTGCTGCAGAAACTGGTTGATGAAAAACACGGGCAGGTGGTTTCTGTCGGGAAACTTGCAGAc                                                                                                                                                                                                                                                  >  1:181378/24‑251 (MQ=255)          aaggaccctttgtccTGGATAAGCGCAACGAACTGTGCGAAATCTCCCGTTAAGAGCATACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTTACAAATCCGGTAACTTCCAACGTACCGGGAACCGTCACGACCTTGCTTGTGAGCCTCCAGCACCGTCCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGcc                                                                                                                                                                                                                                         <  1:252549/237‑1 (MQ=255)                 ctttggccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTAACACATCGGTCGTGTTATCGCTCGTCCGTTTATCAGTGAAAAAGCCGGTAACTTCCCGCGTCCCGGTAACCGTCACGACCTGGCTGTTGCGCCGCCAGCCCCGACCGTGCTGCAGAAAATGGTTGGTGTAAAACACGGGCAGGGGGTTTCTGTCGGGAACATTGCAGACATCTCAGCCAACTGCg                                                                                                                                                                                                                                  >  2:228141/8‑251 (MQ=255)                   ttggccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGTGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCgg                                                                                                                                                                                                                                 <  2:179212/245‑1 (MQ=255)                         tGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGGAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGAGGTTTCTGTCGGTAAAATTGCAGAAATCTACGCCAACTGCGGTATCAcc                                                                                                                                                                                                                          >  1:20637/1‑251 (MQ=255)                            aTAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTTTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGTTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACGGCGGTATCACCaag                                                                                                                                                                                                                       <  1:191547/251‑2 (MQ=255)                            aTAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGAACAACGGCGGCTACAACATCGGACGTGTTATAGCCCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGGACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGGGCTGCAGAAACTGGGTGATGAAAAACACGGTCAGGTGGGTTCTGTCGGTAAAATTGCAGACATATAAGCCAAATGCGGTATCACCa                                                                                                                                                                                                                         >  2:81605/1‑249 (MQ=255)                            aTAAACTCTACGAACTGTGCGAAAGCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCTGGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCaag                                                                                                                                                                                                                       <  1:257808/251‑2 (MQ=255)                                 ctctACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGt                                                                                                                                                                                                                  >  1:223060/1‑251 (MQ=255)                                              gCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAAATTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGAACGTGCAGCAGAAACTGGTTGATGAAAAACACGGTCAGGGGGTTTCTGGCGGGAAAATTGCAGACATCTACGCCAACTGCGGTATCACCACAAAAGGGAAAGCAACTGGc                                                                                                                                                                                                     >  1:262986/1‑251 (MQ=255)                                                  aaTCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACGTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTgg                                                                                                                                                                                                 <  1:133375/251‑1 (MQ=255)                                                     cGCCCGTGAGGAGCTGACCAACGGCGGCTACAAAAGCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGAcg                                                                                                                                                                                              <  2:2307/251‑1 (MQ=255)                                                        ccGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACCAAGCCGGTAAATTCCAGCGTACCGGGAACCGTCACGACATGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGATGATGAAAAACACGGTCAGGTGGCTTCTGTCGGTAAAATTGCAGGCCTCTACGCCAACTGCGGGATCACCAAGAAAGTGAAAGCAACCGGCCTgggacggc                                                                                                                                                                                           >  2:94347/1‑245 (MQ=255)                                                         cGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCt                                                                                                                                                                                          <  1:180664/251‑1 (MQ=255)                                                            gAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACCAAGACGGTAACTTCCAGCGTACCGGTAACCGTCACGACCCGGCTGTTGAGCCGCCAGCACCGAACGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGtt                                                                                                                                                                                       >  2:265075/1‑251 (MQ=255)                                                             aaGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACGGGCCTGGACGCGCTGttt                                                                                                                                                                                      <  1:68690/251‑1 (MQ=255)                                                                    gACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGcca                                                                                                                                                                               <  1:49115/251‑1 (MQ=255)                                                                                  acaacagcGGTCGTGTTATCGCTCGTCCTGTTATCGTTGACAAAGCCGGTAACTGCCAGCGTACCGGTAACCGTCACGCCCTGGCTGTTAAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAgaga                                                                                                                                                                   <  2:12368/242‑1 (MQ=255)                                                                                            gcGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCgg                                                                                                                                                       <  1:265075/250‑1 (MQ=255)                                                                                                         cGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCAt                                                                                                                                          >  1:246063/1‑251 (MQ=255)                                                                                                          gTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGCGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAAAACCATc                                                                                                                                         >  2:5093/1‑251 (MQ=255)                                                                                                                    cggtgACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCAcc                                                                                                                                >  2:199780/5‑250 (MQ=255)                                                                                                                       tgACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGCAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGAGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTATTCACCAACt                                                                                                                            >  2:250661/2‑251 (MQ=255)                                                                                                                             atccGGTAACGTCCAGCGTACGGGGAGCCCGCACGGCCGGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTCTCTGGCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAACAAAGTGAAAGCAACTGGCCGGGACGCGCTGTTTGACGCCACCATCAAATCGATGAAAGAACCGTATGATAACACCAGCTTCTTCCTCAACTCCGttc                                                                                                                      <  2:18719/249‑2 (MQ=255)                                                                                                                                  gTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGAGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCCCCATCAAAGAGATGAAAGAAGCGGGTGATAACACCCTCGTCTTCACCAACTTCGTTGACTTc                                                                                                                 >  2:263221/1‑251 (MQ=255)                                                                                                                                          cAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTc                                                                                                         >  1:125134/1‑251 (MQ=255)                                                                                                                                               tACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTggg                                                                                                    >  1:251146/1‑251 (MQ=255)                                                                                                                                                        ccGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGACAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGAGGGTGCTACCACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGTCCCCCGTc                                                                                           >  2:225019/1‑251 (MQ=255)                                                                                                                                                         cGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTcg                                                                                          <  2:252782/251‑1 (MQ=255)                                                                                                                                                            cACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGa                                                                                       <  1:8623/251‑1 (MQ=255)                                                                                                                                                                aCCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACATCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTc                                                                                   >  1:220602/1‑251 (MQ=255)                                                                                                                                                                aCCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACGGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTc                                                                                   <  2:223060/251‑1 (MQ=255)                                                                                                                                                                            aGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGct                                                                       >  1:194916/1‑250 (MQ=255)                                                                                                                                                                                          accgTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGagct                                                         <  1:94347/251‑4 (MQ=255)                                                                                                                                                                                                     gAAACGGGTTGATGAAAAACACGGTCAGGTGGGTTCTGTCGGTAAAAGTGCAGACATCGACGCCATCTGCGGTATCACCAAGAAATTGAAAGCAACTGGCCTGTACGCGCTGTTTGCCGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGAccgcc                                              <  2:220602/251‑1 (MQ=255)                                                                                                                                                                                                        acgggtggataaaaaaCACGGTCAGGTGTTTTCGGTCGGTAAAATTGCAGACATCTACGCCAACGGCGGTACCACCAAGAAAGGAAAAGCAACGGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGCCTTCACCAACTGCGTTGACTGCGACTCTTCCGGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTc                                           <  2:48766/240‑1 (MQ=255)                                                                                                                                                                                                           ggTTGATGAAAAACACGGTCAGGTGGTTTGGGTCGGTAAAATTTCATACATCTACATCAACTGCGGTATCCCCAAGAAAGTCAAAGCATCTGGCCTGTACGCGCTGTTTGACGCCGCCACCAAATAGATGAAAGAAGCGGGTTATAACACCATCGTCTTCACCAACTTCGTTGACTGCGACGCTTCCTGGGGCCACCTTCGCGTCGTTGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCt                                          <  1:164007/249‑1 (MQ=255)                                                                                                                                                                                                                atgaAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAg                                   <  1:42100/251‑1 (MQ=255)                                                                                                                                                                                                                      aaCACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATg                             >  1:94385/1‑251 (MQ=255)                                                                                                                                                                                                                            gtcAGGTGGTTTCTGTCGGTAAAATTGCAGACGTCTACGCCCACGGCGGTATCACCACGAAAGTGAAAGCAACTGGCCTGGCCGCGCTGTTTGACGCCACCATCAAAGAGCTGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGCCGCCGGTTATGCTGCGGGTCTGGGGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTctg                       >  2:268417/3‑251 (MQ=255)                                                                                                                                                                                                                                  tggtTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTgcgc                 >  2:122444/1‑251 (MQ=255)                                                                                                                                                                                                                                                 aaaTTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAACGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTTTGCTGCGCGATGCCGACATCCTg  >  2:169248/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    CCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATGTCTCTGCTGCGCGATGACGACATCCTG  >  NC_000913/4620138‑4620628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑