Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,358	C→T	G252G (GGC→GGT)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,358	0	C	T	95.5%	126.9 / NA	45	G252G (GGC→GGT)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (21/21);  minor base G (2/0);  total (24/21)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATG  >  NC_000913/4620123‑4620601                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    ccGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTg                                                                                                                                                                                                                                        <  1:215306/249‑1 (MQ=255)     gtTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGTGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGt                                                                                                                                                                                                                                   >  1:179212/1‑250 (MQ=255)     gtTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTTTGTCggg                                                                                                                                                                                                                                   >  2:8623/1‑249 (MQ=255)       tCCAGATTGCCTGCCACGAAGGAACATTTGGCCTGGATAAACTCTACGCACTCTGCGAAATCGCCCGTGAAGAGATGACCCACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCCTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAAACGTCACGGCCTCGCTGTTGAGCCGACAGCCCCGACCGTGCTGCAAAAACCGGTTGCTGAAAGACACGGTCCGGAGGGTTCTGTCgggaa                                                                                                                                                                                                                                 >  2:257808/1‑247 (MQ=255)         cAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGTCGGCTACAACATCGGTCGTGTTATCACTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTAACGGTAACCGTCCCGACCTGGCTGTTGAGCCGCCAGCACCGACCCTGCTGCAGAAACATGTTGGTGAAAAACACGGTCAGGAGGGTTCTGTCGGTAAAAt                                                                                                                                                                                                                              >  2:206977/1‑251 (MQ=255)           gATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGGGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGGTTCTGTCGGTAAAATTg                                                                                                                                                                                                                            >  2:68690/1‑251 (MQ=255)                cctgccacgacggaacctttgcccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACCACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGAACAAGCCGGTAACTTCCAGCGTACCGGTAACCGCCACGCCCTGGCTGTTGAGCCGCCATCACCGCCCGTGCTGCAGAAACTGGTTGATGAAAAACACGGGCAGGTGGTTTCTGTCGGGAAACTTGCAGAc                                                                                                                                                                                                                       >  1:181378/24‑251 (MQ=255)                         aaggaccctttgtccTGGATAAGCGCAACGAACTGTGCGAAATCTCCCGTTAAGAGCATACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTTACAAATCCGGTAACTTCCAACGTACCGGGAACCGTCACGACCTTGCTTGTGAGCCTCCAGCACCGTCCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGcc                                                                                                                                                                                                              <  1:252549/237‑1 (MQ=255)                                ctttggccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTAACACATCGGTCGTGTTATCGCTCGTCCGTTTATCAGTGAAAAAGCCGGTAACTTCCCGCGTCCCGGTAACCGTCACGACCTGGCTGTTGCGCCGCCAGCCCCGACCGTGCTGCAGAAAATGGTTGGTGTAAAACACGGGCAGGGGGTTTCTGTCGGGAACATTGCAGACATCTCAGCCAACTGCg                                                                                                                                                                                                       >  2:228141/8‑251 (MQ=255)                                  ttggccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGTGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCgg                                                                                                                                                                                                      <  2:179212/245‑1 (MQ=255)                                        tGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGGAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGAGGTTTCTGTCGGTAAAATTGCAGAAATCTACGCCAACTGCGGTATCAcc                                                                                                                                                                                               >  1:20637/1‑251 (MQ=255)                                           aTAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTTTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGTTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACGGCGGTATCACCaag                                                                                                                                                                                            <  1:191547/251‑2 (MQ=255)                                           aTAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGAACAACGGCGGCTACAACATCGGACGTGTTATAGCCCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGGACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGGGCTGCAGAAACTGGGTGATGAAAAACACGGTCAGGTGGGTTCTGTCGGTAAAATTGCAGACATATAAGCCAAATGCGGTATCACCa                                                                                                                                                                                              >  2:81605/1‑249 (MQ=255)                                           aTAAACTCTACGAACTGTGCGAAAGCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCTGGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCaag                                                                                                                                                                                            <  1:257808/251‑2 (MQ=255)                                                ctctACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGt                                                                                                                                                                                       >  1:223060/1‑251 (MQ=255)                                                             gCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAAATTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGAACGTGCAGCAGAAACTGGTTGATGAAAAACACGGTCAGGGGGTTTCTGGCGGGAAAATTGCAGACATCTACGCCAACTGCGGTATCACCACAAAAGGGAAAGCAACTGGc                                                                                                                                                                          >  1:262986/1‑251 (MQ=255)                                                                 aaTCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACGTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTgg                                                                                                                                                                      <  1:133375/251‑1 (MQ=255)                                                                    cGCCCGTGAGGAGCTGACCAACGGCGGCTACAAAAGCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGAcg                                                                                                                                                                   <  2:2307/251‑1 (MQ=255)                                                                       ccGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACCAAGCCGGTAAATTCCAGCGTACCGGGAACCGTCACGACATGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGATGATGAAAAACACGGTCAGGTGGCTTCTGTCGGTAAAATTGCAGGCCTCTACGCCAACTGCGGGATCACCAAGAAAGTGAAAGCAACCGGCCTgggacggc                                                                                                                                                                >  2:94347/1‑245 (MQ=255)                                                                        cGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCt                                                                                                                                                               <  1:180664/251‑1 (MQ=255)                                                                           gAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACCAAGACGGTAACTTCCAGCGTACCGGTAACCGTCACGACCCGGCTGTTGAGCCGCCAGCACCGAACGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGtt                                                                                                                                                            >  2:265075/1‑251 (MQ=255)                                                                            aaGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACGGGCCTGGACGCGCTGttt                                                                                                                                                           <  1:68690/251‑1 (MQ=255)                                                                                   gACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGcca                                                                                                                                                    <  1:49115/251‑1 (MQ=255)                                                                                                 acaacagcGGTCGTGTTATCGCTCGTCCTGTTATCGTTGACAAAGCCGGTAACTGCCAGCGTACCGGTAACCGTCACGCCCTGGCTGTTAAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAgaga                                                                                                                                        <  2:12368/242‑1 (MQ=255)                                                                                                           gcGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCgg                                                                                                                            <  1:265075/250‑1 (MQ=255)                                                                                                                        cGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCAt                                                                                                               >  1:246063/1‑251 (MQ=255)                                                                                                                         gTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGCGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAAAACCATc                                                                                                              >  2:5093/1‑251 (MQ=255)                                                                                                                                   cggtgACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCAcc                                                                                                     >  2:199780/5‑250 (MQ=255)                                                                                                                                      tgACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGCAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGAGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTATTCACCAACt                                                                                                 >  2:250661/2‑251 (MQ=255)                                                                                                                                            atccGGTAACGTCCAGCGTACGGGGAGCCCGCACGGCCGGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTCTCTGGCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAACAAAGTGAAAGCAACTGGCCGGGACGCGCTGTTTGACGCCACCATCAAATCGATGAAAGAACCGTATGATAACACCAGCTTCTTCCTCAACTCCGttc                                                                                           <  2:18719/249‑2 (MQ=255)                                                                                                                                                 gTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGAGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCCCCATCAAAGAGATGAAAGAAGCGGGTGATAACACCCTCGTCTTCACCAACTTCGTTGACTTc                                                                                      >  2:263221/1‑251 (MQ=255)                                                                                                                                                         cAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTc                                                                              >  1:125134/1‑251 (MQ=255)                                                                                                                                                              tACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTggg                                                                         >  1:251146/1‑251 (MQ=255)                                                                                                                                                                       ccGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGACAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGAGGGTGCTACCACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGTCCCCCGTc                                                                >  2:225019/1‑251 (MQ=255)                                                                                                                                                                        cGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTcg                                                               <  2:252782/251‑1 (MQ=255)                                                                                                                                                                           cACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGa                                                            <  1:8623/251‑1 (MQ=255)                                                                                                                                                                               aCCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACATCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTc                                                        >  1:220602/1‑251 (MQ=255)                                                                                                                                                                               aCCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACGGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTc                                                        <  2:223060/251‑1 (MQ=255)                                                                                                                                                                                           aGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGct                                            >  1:194916/1‑250 (MQ=255)                                                                                                                                                                                                         accgTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGagct                              <  1:94347/251‑4 (MQ=255)                                                                                                                                                                                                                    gAAACGGGTTGATGAAAAACACGGTCAGGTGGGTTCTGTCGGTAAAAGTGCAGACATCGACGCCATCTGCGGTATCACCAAGAAATTGAAAGCAACTGGCCTGTACGCGCTGTTTGCCGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGAccgcc                   <  2:220602/251‑1 (MQ=255)                                                                                                                                                                                                                       acgggtggataaaaaaCACGGTCAGGTGTTTTCGGTCGGTAAAATTGCAGACATCTACGCCAACGGCGGTACCACCAAGAAAGGAAAAGCAACGGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGCCTTCACCAACTGCGTTGACTGCGACTCTTCCGGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTc                <  2:48766/240‑1 (MQ=255)                                                                                                                                                                                                                          ggTTGATGAAAAACACGGTCAGGTGGTTTGGGTCGGTAAAATTTCATACATCTACATCAACTGCGGTATCCCCAAGAAAGTCAAAGCATCTGGCCTGTACGCGCTGTTTGACGCCGCCACCAAATAGATGAAAGAAGCGGGTTATAACACCATCGTCTTCACCAACTTCGTTGACTGCGACGCTTCCTGGGGCCACCTTCGCGTCGTTGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCt               <  1:164007/249‑1 (MQ=255)                                                                                                                                                                                                                               atgaAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAg        <  1:42100/251‑1 (MQ=255)                                                                                                                                                                                                                                     aaCACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCTGCGGGTCTGGAGCTGTTCGACCGCCGTCTGCCGGAGCTGATg  >  1:94385/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    CCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATCGTCTTCACCAACTTCGTTGACTTCGACTCTTCCTGGGGCCACCGTCGCGACGTCGCCGGTTATGCCGCGGGTCTGGAACTGTTCGACCGCCGTCTGCCGGAGCTGATG  >  NC_000913/4620123‑4620601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑