Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,256	C→T	G218G (GGC→GGT)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,256	0	C	T	96.8%	87.3 / NA	31	G218G (GGC→GGT)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (17/13);  minor base G (1/0);  total (18/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATC  >  NC_000913/4620034‑4620493                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              cTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGAGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCg                                                                                                                                                                                                                   >  1:180835/1‑251 (MQ=255)   gCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGt                                                                                                                                                                                                                 >  1:252782/1‑251 (MQ=255)    ccACTCTTCCGGTACGTTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCTCTCGTCCGTTTATCCGTGTCCAAGCCGGTAACTTCCAGCGTACCGGt                                                                                                                                                                                                                 >  2:203930/1‑250 (MQ=255)                       aTTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGt                                                                                                                                                                                             <  1:245644/251‑1 (MQ=255)                                                                                          ccGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTg                                                                                                                            <  1:215306/249‑1 (MQ=255)                                                                                              gtTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGTGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGt                                                                                                                       >  1:179212/1‑250 (MQ=255)                                                                                              gtTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTTTGTCggg                                                                                                                       >  2:8623/1‑249 (MQ=255)                                                                                                tCCAGATTGCCTGCCACGAAGGAACATTTGGCCTGGATAAACTCTACGCACTCTGCGAAATCGCCCGTGAAGAGATGACCCACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCCTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAAACGTCACGGCCTCGCTGTTGAGCCGACAGCCCCGACCGTGCTGCAAAAACCGGTTGCTGAAAGACACGGTCCGGAGGGTTCTGTCgggaa                                                                                                                     >  2:257808/1‑247 (MQ=255)                                                                                                  cAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGTCGGCTACAACATCGGTCGTGTTATCACTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTAACGGTAACCGTCCCGACCTGGCTGTTGAGCCGCCAGCACCGACCCTGCTGCAGAAACATGTTGGTGAAAAACACGGTCAGGAGGGTTCTGTCGGTAAAAt                                                                                                                  >  2:206977/1‑251 (MQ=255)                                                                                                    gATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGGGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGGTTCTGTCGGTAAAATTg                                                                                                                >  2:68690/1‑251 (MQ=255)                                                                                                         cctgccacgacggaacctttgcccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACCACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGAACAAGCCGGTAACTTCCAGCGTACCGGTAACCGCCACGCCCTGGCTGTTGAGCCGCCATCACCGCCCGTGCTGCAGAAACTGGTTGATGAAAAACACGGGCAGGTGGTTTCTGTCGGGAAACTTGCAGAc                                                                                                           >  1:181378/24‑251 (MQ=255)                                                                                                                  aaggaccctttgtccTGGATAAGCGCAACGAACTGTGCGAAATCTCCCGTTAAGAGCATACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTTACAAATCCGGTAACTTCCAACGTACCGGGAACCGTCACGACCTTGCTTGTGAGCCTCCAGCACCGTCCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGcc                                                                                                  <  1:252549/237‑1 (MQ=255)                                                                                                                         ctttggccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTAACACATCGGTCGTGTTATCGCTCGTCCGTTTATCAGTGAAAAAGCCGGTAACTTCCCGCGTCCCGGTAACCGTCACGACCTGGCTGTTGCGCCGCCAGCCCCGACCGTGCTGCAGAAAATGGTTGGTGTAAAACACGGGCAGGGGGTTTCTGTCGGGAACATTGCAGACATCTCAGCCAACTGCg                                                                                           >  2:228141/8‑251 (MQ=255)                                                                                                                           ttggccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGTGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCgg                                                                                          <  2:179212/245‑1 (MQ=255)                                                                                                                                 tGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGGAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGAGGTTTCTGTCGGTAAAATTGCAGAAATCTACGCCAACTGCGGTATCAcc                                                                                   >  1:20637/1‑251 (MQ=255)                                                                                                                                    aTAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTTTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGTTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACGGCGGTATCACCaag                                                                                <  1:191547/251‑2 (MQ=255)                                                                                                                                    aTAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGAACAACGGCGGCTACAACATCGGACGTGTTATAGCCCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGGACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGGGCTGCAGAAACTGGGTGATGAAAAACACGGTCAGGTGGGTTCTGTCGGTAAAATTGCAGACATATAAGCCAAATGCGGTATCACCa                                                                                  >  2:81605/1‑249 (MQ=255)                                                                                                                                    aTAAACTCTACGAACTGTGCGAAAGCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCTGGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCaag                                                                                <  1:257808/251‑2 (MQ=255)                                                                                                                                         ctctACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGt                                                                           >  1:223060/1‑251 (MQ=255)                                                                                                                                                      gCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAAATTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGAACGTGCAGCAGAAACTGGTTGATGAAAAACACGGTCAGGGGGTTTCTGGCGGGAAAATTGCAGACATCTACGCCAACTGCGGTATCACCACAAAAGGGAAAGCAACTGGc                                                              >  1:262986/1‑251 (MQ=255)                                                                                                                                                          aaTCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACGTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTgg                                                          <  1:133375/251‑1 (MQ=255)                                                                                                                                                             cGCCCGTGAGGAGCTGACCAACGGCGGCTACAAAAGCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGAcg                                                       <  2:2307/251‑1 (MQ=255)                                                                                                                                                                ccGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACCAAGCCGGTAAATTCCAGCGTACCGGGAACCGTCACGACATGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGATGATGAAAAACACGGTCAGGTGGCTTCTGTCGGTAAAATTGCAGGCCTCTACGCCAACTGCGGGATCACCAAGAAAGTGAAAGCAACCGGCCTgggacggc                                                    >  2:94347/1‑245 (MQ=255)                                                                                                                                                                 cGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCt                                                   <  1:180664/251‑1 (MQ=255)                                                                                                                                                                    gAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACCAAGACGGTAACTTCCAGCGTACCGGTAACCGTCACGACCCGGCTGTTGAGCCGCCAGCACCGAACGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGtt                                                >  2:265075/1‑251 (MQ=255)                                                                                                                                                                     aaGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACGGGCCTGGACGCGCTGttt                                               <  1:68690/251‑1 (MQ=255)                                                                                                                                                                            gACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGcca                                        <  1:49115/251‑1 (MQ=255)                                                                                                                                                                                          acaacagcGGTCGTGTTATCGCTCGTCCTGTTATCGTTGACAAAGCCGGTAACTGCCAGCGTACCGGTAACCGTCACGCCCTGGCTGTTAAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAgaga                            <  2:12368/242‑1 (MQ=255)                                                                                                                                                                                                    gcGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCgg                <  1:265075/250‑1 (MQ=255)                                                                                                                                                                                                                 cGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCAt   >  1:246063/1‑251 (MQ=255)                                                                                                                                                                                                                  gTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGCGAAAGCAACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAAAACCATc  >  2:5093/1‑251 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              CTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCACCATCAAAGAGATGAAAGAAGCGGGTGATAACACCATC  >  NC_000913/4620034‑4620493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑