Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,223	T→C	N207N (AAT→AAC)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,223	0	T	C	96.8%	77.9 / ‑6.0	31	N207N (AAT→AAC)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (18/12);  minor base A (0/1);  total (18/13)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCA  >  NC_000913/4619983‑4620455                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         gctgctGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCgtg                                                                                                                                                                                                                                <  1:56251/251‑1 (MQ=255)    gctgGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAAATGCCACTCTTACGGTACGGTCATTCTGGATCAGCTGGGAGAAGAGCACATGAAAACCGGCAAGCCGCTTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACATTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACCACATCggccgggtt                                                                                                                                                                                                                              >  1:18719/1‑243 (MQ=255)              cTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATAGCTCGTcc                                                                                                                                                                                                                   >  1:226982/1‑251 (MQ=255)                        gcgcTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCggt                                                                                                                                                                                                         <  1:243515/251‑2 (MQ=255)                                                    cTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGAGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCg                                                                                                                                                                             >  1:180835/1‑251 (MQ=255)                                                      gCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGt                                                                                                                                                                           >  1:252782/1‑251 (MQ=255)                                                       ccACTCTTCCGGTACGTTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCTCTCGTCCGTTTATCCGTGTCCAAGCCGGTAACTTCCAGCGTACCGGt                                                                                                                                                                           >  2:203930/1‑250 (MQ=255)                                                                          aTTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGt                                                                                                                                                       <  1:245644/251‑1 (MQ=255)                                                                                                                                             ccGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTg                                                                                      <  1:215306/249‑1 (MQ=255)                                                                                                                                                 gtTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGTGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGt                                                                                 >  1:179212/1‑250 (MQ=255)                                                                                                                                                 gtTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTTTGTCggg                                                                                 >  2:8623/1‑249 (MQ=255)                                                                                                                                                   tCCAGATTGCCTGCCACGAAGGAACATTTGGCCTGGATAAACTCTACGCACTCTGCGAAATCGCCCGTGAAGAGATGACCCACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCCTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAAACGTCACGGCCTCGCTGTTGAGCCGACAGCCCCGACCGTGCTGCAAAAACCGGTTGCTGAAAGACACGGTCCGGAGGGTTCTGTCgggaa                                                                               >  2:257808/1‑247 (MQ=255)                                                                                                                                                     cAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGTCGGCTACAACATCGGTCGTGTTATCACTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTAACGGTAACCGTCCCGACCTGGCTGTTGAGCCGCCAGCACCGACCCTGCTGCAGAAACATGTTGGTGAAAAACACGGTCAGGAGGGTTCTGTCGGTAAAAt                                                                            >  2:206977/1‑251 (MQ=255)                                                                                                                                                       gATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGGGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGGTTCTGTCGGTAAAATTg                                                                          >  2:68690/1‑251 (MQ=255)                                                                                                                                                            cctgccacgacggaacctttgcccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACCACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGAACAAGCCGGTAACTTCCAGCGTACCGGTAACCGCCACGCCCTGGCTGTTGAGCCGCCATCACCGCCCGTGCTGCAGAAACTGGTTGATGAAAAACACGGGCAGGTGGTTTCTGTCGGGAAACTTGCAGAc                                                                     >  1:181378/24‑251 (MQ=255)                                                                                                                                                                     aaggaccctttgtccTGGATAAGCGCAACGAACTGTGCGAAATCTCCCGTTAAGAGCATACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTTACAAATCCGGTAACTTCCAACGTACCGGGAACCGTCACGACCTTGCTTGTGAGCCTCCAGCACCGTCCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGcc                                                            <  1:252549/237‑1 (MQ=255)                                                                                                                                                                            ctttggccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTAACACATCGGTCGTGTTATCGCTCGTCCGTTTATCAGTGAAAAAGCCGGTAACTTCCCGCGTCCCGGTAACCGTCACGACCTGGCTGTTGCGCCGCCAGCCCCGACCGTGCTGCAGAAAATGGTTGGTGTAAAACACGGGCAGGGGGTTTCTGTCGGGAACATTGCAGACATCTCAGCCAACTGCg                                                     >  2:228141/8‑251 (MQ=255)                                                                                                                                                                              ttggccTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGTGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCgg                                                    <  2:179212/245‑1 (MQ=255)                                                                                                                                                                                    tGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGGAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGAGGTTTCTGTCGGTAAAATTGCAGAAATCTACGCCAACTGCGGTATCAcc                                             >  1:20637/1‑251 (MQ=255)                                                                                                                                                                                       aTAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTTTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGTTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACGGCGGTATCACCaag                                          <  1:191547/251‑2 (MQ=255)                                                                                                                                                                                       aTAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGAACAACGGCGGCTACAACATCGGACGTGTTATAGCCCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGGACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGGGCTGCAGAAACTGGGTGATGAAAAACACGGTCAGGTGGGTTCTGTCGGTAAAATTGCAGACATATAAGCCAAATGCGGTATCACCa                                            >  2:81605/1‑249 (MQ=255)                                                                                                                                                                                       aTAAACTCTACGAACTGTGCGAAAGCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCTGGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCaag                                          <  1:257808/251‑2 (MQ=255)                                                                                                                                                                                            ctctACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGt                                     >  1:223060/1‑251 (MQ=255)                                                                                                                                                                                                         gCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAAATTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGAACGTGCAGCAGAAACTGGTTGATGAAAAACACGGTCAGGGGGTTTCTGGCGGGAAAATTGCAGACATCTACGCCAACTGCGGTATCACCACAAAAGGGAAAGCAACTGGc                        >  1:262986/1‑251 (MQ=255)                                                                                                                                                                                                             aaTCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACGTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTgg                    <  1:133375/251‑1 (MQ=255)                                                                                                                                                                                                                cGCCCGTGAGGAGCTGACCAACGGCGGCTACAAAAGCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGAcg                 <  2:2307/251‑1 (MQ=255)                                                                                                                                                                                                                   ccGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACCAAGCCGGTAAATTCCAGCGTACCGGGAACCGTCACGACATGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGATGATGAAAAACACGGTCAGGTGGCTTCTGTCGGTAAAATTGCAGGCCTCTACGCCAACTGCGGGATCACCAAGAAAGTGAAAGCAACCGGCCTgggacggc              >  2:94347/1‑245 (MQ=255)                                                                                                                                                                                                                    cGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCt             <  1:180664/251‑1 (MQ=255)                                                                                                                                                                                                                       gAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACCAAGACGGTAACTTCCAGCGTACCGGTAACCGTCACGACCCGGCTGTTGAGCCGCCAGCACCGAACGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGtt          >  2:265075/1‑251 (MQ=255)                                                                                                                                                                                                                        aaGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACGGGCCTGGACGCGCTGttt         <  1:68690/251‑1 (MQ=255)                                                                                                                                                                                                                               gACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGTAAAATTGCAGACATCTACGCCAACTGCGGTATCACCAAGAAAGTGAAAGCAACTGGCCTGGACGCGCTGTTTGACGcca  <  1:49115/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         GCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGACATCTACGCCAACTGCGGTATCACCAAAAAAGTGAAAGCGACTGGCCTGGACGCGCTGTTTGACGCCA  >  NC_000913/4619983‑4620455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑