Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,160	T→C	G186G (GGT→GGC)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,160	0	T	C	95.5%	63.6 / NA	22	G186G (GGT→GGC)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (15/6);  total (15/7)
Fisher's exact test for biased strand distribution p-value = 3.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTG  >  NC_000913/4619926‑4620383                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                cccGGTTCTGTTTGAGTGGGGATATTTCCCCAATCACGAAACCAGCTTCCCGCAGGAGCTGCTGGATAACCTGGTCGAACGCGCTAACCGGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTAc                                                                                                                                                                                                                  <  2:180728/250‑1 (MQ=255)               aGTGGGGATATTTCTCCGATCACGACAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGaactc                                                                                                                                                                                                   >  2:215306/1‑248 (MQ=255)                       aacagcacgctcgtcctgtctatcTTCCCGCAAGAGCTGCTGGATAACGTGTTGGAACGCGCGAAGCTGCCGGTGTCGCTCGGTAACTGCCAGTCTGCCGGTACGTTCATGCTGGAGCTGCGGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTGCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGTCTGGATAAACTTTACGAACTGCGTGAAATCGCCCGTGa                                                                                                                                                                                           <  2:126255/228‑1 (MQ=255)                                           cAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCt                                                                                                                                                                       >  1:48766/1‑251 (MQ=255)                                            aGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTACGGTACGGTCATTCTGGATCAGCTGGGCGCAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGa                                                                                                                                                                                           >  1:126255/1‑230 (MQ=255)                                                  ccGCAAGTGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGGGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACaacat                                                                                                                                                                >  2:42100/1‑248 (MQ=255)                                                          gctgctGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCgtg                                                                                                                                                        <  1:56251/251‑1 (MQ=255)                                                             gctgGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAAATGCCACTCTTACGGTACGGTCATTCTGGATCAGCTGGGAGAAGAGCACATGAAAACCGGCAAGCCGCTTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACATTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACCACATCggccgggtt                                                                                                                                                      >  1:18719/1‑243 (MQ=255)                                                                       cTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATAGCTCGTcc                                                                                                                                           >  1:226982/1‑251 (MQ=255)                                                                                 gcgcTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCggt                                                                                                                                 <  1:243515/251‑2 (MQ=255)                                                                                                             cTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGAGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCg                                                                                                     >  1:180835/1‑251 (MQ=255)                                                                                                               gCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGt                                                                                                   >  1:252782/1‑251 (MQ=255)                                                                                                                ccACTCTTCCGGTACGTTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCTCTCGTCCGTTTATCCGTGTCCAAGCCGGTAACTTCCAGCGTACCGGt                                                                                                   >  2:203930/1‑250 (MQ=255)                                                                                                                                   aTTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGt                                                                               <  1:245644/251‑1 (MQ=255)                                                                                                                                       tGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGGCCGTGAAGAGCGGACCAACGGCGTCTACaacat                                                                                                                                                                <  1:219031/166‑4 (MQ=255)                                                                                                                                       tGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACaacat                                                                                                                                                                >  2:219031/1‑163 (MQ=255)                                                                                                                                                                                                      ccGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTg              <  1:215306/249‑1 (MQ=255)                                                                                                                                                                                                          gtTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGTGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGt         >  1:179212/1‑250 (MQ=255)                                                                                                                                                                                                          gtTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTTTGTCggg         >  2:8623/1‑249 (MQ=255)                                                                                                                                                                                                            tCCAGATTGCCTGCCACGAAGGAACATTTGGCCTGGATAAACTCTACGCACTCTGCGAAATCGCCCGTGAAGAGATGACCCACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCCTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAAACGTCACGGCCTCGCTGTTGAGCCGACAGCCCCGACCGTGCTGCAAAAACCGGTTGCTGAAAGACACGGTCCGGAGGGTTCTGTCgggaa       >  2:257808/1‑247 (MQ=255)                                                                                                                                                                                                              cAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGTCGGCTACAACATCGGTCGTGTTATCACTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTAACGGTAACCGTCCCGACCTGGCTGTTGAGCCGCCAGCACCGACCCTGCTGCAGAAACATGTTGGTGAAAAACACGGTCAGGAGGGTTCTGTCGGTAAAAt    >  2:206977/1‑251 (MQ=255)                                                                                                                                                                                                                gATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGGGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGGTTCTGTCGGTAAAATTg  >  2:68690/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                CCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTG  >  NC_000913/4619926‑4620383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑