Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,145	T→C	H181H (CAT→CAC)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,145	0	T	C	100.0%	75.8 / NA	24	H181H (CAT→CAC)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/9);  total (15/9)

TGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTG  >  NC_000913/4619909‑4620383                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               tgtgaaagtgcctgtggcgcGGTTCTGGGTGAGTGGGTATATGTCTCCGATCTCGAAAACAGCTGCCCGCAAGTGCTGCTGAATAAACTGGTCGAACGCGCTAATGTGCCGGGTTACCACGGTAACTGCCGCTCTTCCTGTGCGGTCATTCTGGATCATCGGTGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCGCCGCTGACGCCGTGTTCCAGATTGCCTGCCACGAAGGAAcctttg                                                                                                                                                                                                                                   <  1:157853/231‑6 (MQ=255)    gAAATCGCCGGTGTCCCGGTTCTTTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTTCCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGTCTCCGTGTTCCAGATTGCCTGCCACGAAGGAAcctttggcct                                                                                                                                                                                                                               <  1:231067/251‑10 (MQ=255)                  cccGGTTCTGTTTGAGTGGGGATATTTCCCCAATCACGAAACCAGCTTCCCGCAGGAGCTGCTGGATAACCTGGTCGAACGCGCTAACCGGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTAc                                                                                                                                                                                                                  <  2:180728/250‑1 (MQ=255)                                aGTGGGGATATTTCTCCGATCACGACAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGaactc                                                                                                                                                                                                   >  2:215306/1‑248 (MQ=255)                                        aacagcacgctcgtcctgtctatcTTCCCGCAAGAGCTGCTGGATAACGTGTTGGAACGCGCGAAGCTGCCGGTGTCGCTCGGTAACTGCCAGTCTGCCGGTACGTTCATGCTGGAGCTGCGGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTGCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGTCTGGATAAACTTTACGAACTGCGTGAAATCGCCCGTGa                                                                                                                                                                                           <  2:126255/228‑1 (MQ=255)                                                            cAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCt                                                                                                                                                                       >  1:48766/1‑251 (MQ=255)                                                             aGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTACGGTACGGTCATTCTGGATCAGCTGGGCGCAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGa                                                                                                                                                                                           >  1:126255/1‑230 (MQ=255)                                                                   ccGCAAGTGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGGGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACaacat                                                                                                                                                                >  2:42100/1‑248 (MQ=255)                                                                           gctgctGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCgtg                                                                                                                                                        <  1:56251/251‑1 (MQ=255)                                                                              gctgGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAAATGCCACTCTTACGGTACGGTCATTCTGGATCAGCTGGGAGAAGAGCACATGAAAACCGGCAAGCCGCTTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACATTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACCACATCggccgggtt                                                                                                                                                      >  1:18719/1‑243 (MQ=255)                                                                                        cTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATAGCTCGTcc                                                                                                                                           >  1:226982/1‑251 (MQ=255)                                                                                                  gcgcTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCggt                                                                                                                                 <  1:243515/251‑2 (MQ=255)                                                                                                                              cTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGAGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCg                                                                                                     >  1:180835/1‑251 (MQ=255)                                                                                                                                gCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGt                                                                                                   >  1:252782/1‑251 (MQ=255)                                                                                                                                 ccACTCTTCCGGTACGTTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCTCTCGTCCGTTTATCCGTGTCCAAGCCGGTAACTTCCAGCGTACCGGt                                                                                                   >  2:203930/1‑250 (MQ=255)                                                                                                                                                    aTTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGt                                                                               <  1:245644/251‑1 (MQ=255)                                                                                                                                                        tGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGGCCGTGAAGAGCGGACCAACGGCGTCTACaacat                                                                                                                                                                <  1:219031/166‑4 (MQ=255)                                                                                                                                                        tGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACaacat                                                                                                                                                                >  2:219031/1‑163 (MQ=255)                                                                                                                                                                                                                       ccGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTg              <  1:215306/249‑1 (MQ=255)                                                                                                                                                                                                                           gtTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGTGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTCTGTCGGt         >  1:179212/1‑250 (MQ=255)                                                                                                                                                                                                                           gtTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGTTTTTGTCggg         >  2:8623/1‑249 (MQ=255)                                                                                                                                                                                                                             tCCAGATTGCCTGCCACGAAGGAACATTTGGCCTGGATAAACTCTACGCACTCTGCGAAATCGCCCGTGAAGAGATGACCCACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCCTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAAACGTCACGGCCTCGCTGTTGAGCCGACAGCCCCGACCGTGCTGCAAAAACCGGTTGCTGAAAGACACGGTCCGGAGGGTTCTGTCgggaa       >  2:257808/1‑247 (MQ=255)                                                                                                                                                                                                                               cAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGTCGGCTACAACATCGGTCGTGTTATCACTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTAACGGTAACCGTCCCGACCTGGCTGTTGAGCCGCCAGCACCGACCCTGCTGCAGAAACATGTTGGTGAAAAACACGGTCAGGAGGGTTCTGTCGGTAAAAt    >  2:206977/1‑251 (MQ=255)                                                                                                                                                                                                                                 gATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGGGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGTCAGGTGGGTTCTGTCGGTAAAATTg  >  2:68690/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               TGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTG  >  NC_000913/4619909‑4620383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑