Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,067	A→G	Q155Q (CAA→CAG)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,067	0	A	G	95.8%	73.0 / NA	24	Q155Q (CAA→CAG)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (15/8);  minor base T (0/1);  total (15/9)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT  >  NC_000913/4619821‑4620306                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                ggcggGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGAGAACGCAATGTCATCCGGTAAAGATACACCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGGGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGTGCTGCTGGCTAAACTGGTCGAACGCGCTAATCTGCCGGGTTGTCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGGTCAGCTgg                                                                                                                                                                                                                                             >  2:180664/1‑251 (MQ=255)                                           aTGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCt                                                                                                                                                                                                   >  1:180728/1‑251 (MQ=255)                                                      aaaTGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGAAAATCGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACTCGCTAATCTGCCGGGTGTCCGCGGTAACTGCCGCTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTcc                                                                                                                                                                                        <  1:125120/251‑1 (MQ=255)                                                          gTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCgtgt                                                                                                                                                                                    >  1:12368/1‑251 (MQ=255)                                                          gTCATCCGGGAACGATACCCCGTCTGGTCACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCCAGCCGATTTTCTATACCTCCGCTGCCTCCgtgt                                                                                                                                                                                    >  2:191547/1‑251 (MQ=255)                                                                     aaGATACCCCGTCTGGTAACTGGGAAATCGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGCTGTTCTATACCTCCGCTGACTCCGTGTTCCAGATtgcc                                                                                                                                                                         >  1:88532/1‑251 (MQ=255)                                                                                         tgtgaaagtgcctgtggcgcGGTTCTGGGTGAGTGGGTATATGTCTCCGATCTCGAAAACAGCTGCCCGCAAGTGCTGCTGAATAAACTGGTCGAACGCGCTAATGTGCCGGGTTACCACGGTAACTGCCGCTCTTCCTGTGCGGTCATTCTGGATCATCGGTGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCGCCGCTGACGCCGTGTTCCAGATTGCCTGCCACGAAGGAAcctttg                                                                                                                                                      <  1:157853/231‑6 (MQ=255)                                                                                            gAAATCGCCGGTGTCCCGGTTCTTTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTTCCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGTCTCCGTGTTCCAGATTGCCTGCCACGAAGGAAcctttggcct                                                                                                                                                  <  1:231067/251‑10 (MQ=255)                                                                                                          cccGGTTCTGTTTGAGTGGGGATATTTCCCCAATCACGAAACCAGCTTCCCGCAGGAGCTGCTGGATAACCTGGTCGAACGCGCTAACCGGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTAc                                                                                                                                     <  2:180728/250‑1 (MQ=255)                                                                                                                        aGTGGGGATATTTCTCCGATCACGACAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGaactc                                                                                                                      >  2:215306/1‑248 (MQ=255)                                                                                                                                aacagcacgctcgtcctgtctatcTTCCCGCAAGAGCTGCTGGATAACGTGTTGGAACGCGCGAAGCTGCCGGTGTCGCTCGGTAACTGCCAGTCTGCCGGTACGTTCATGCTGGAGCTGCGGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTGCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGTCTGGATAAACTTTACGAACTGCGTGAAATCGCCCGTGa                                                                                                              <  2:126255/228‑1 (MQ=255)                                                                                                                                                    cAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCt                                                                                          >  1:48766/1‑251 (MQ=255)                                                                                                                                                     aGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTACGGTACGGTCATTCTGGATCAGCTGGGCGCAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGa                                                                                                              >  1:126255/1‑230 (MQ=255)                                                                                                                                                           ccGCAAGTGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGGGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACaacat                                                                                   >  2:42100/1‑248 (MQ=255)                                                                                                                                                                   gctgctGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCgtg                                                                           <  1:56251/251‑1 (MQ=255)                                                                                                                                                                      gctgGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAAATGCCACTCTTACGGTACGGTCATTCTGGATCAGCTGGGAGAAGAGCACATGAAAACCGGCAAGCCGCTTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACATTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACCACATCggccgggtt                                                                         >  1:18719/1‑243 (MQ=255)                                                                                                                                                                                cTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATAGCTCGTcc                                                              >  1:226982/1‑251 (MQ=255)                                                                                                                                                                                          gcgcTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCggt                                                    <  1:243515/251‑2 (MQ=255)                                                                                                                                                                                                                      cTGCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGAGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCg                        >  1:180835/1‑251 (MQ=255)                                                                                                                                                                                                                        gCCACTCTTCCGGTACGGTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGt                      >  1:252782/1‑251 (MQ=255)                                                                                                                                                                                                                         ccACTCTTCCGGTACGTTCATTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCTCTCGTCCGTTTATCCGTGTCCAAGCCGGTAACTTCCAGCGTACCGGt                      >  2:203930/1‑250 (MQ=255)                                                                                                                                                                                                                                            aTTCTGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAACATCGGTCGTGTTATCGCTCGTCCGTTTATCGGTGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGt  <  1:245644/251‑1 (MQ=255)                                                                                                                                                                                                                                                tGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGGCCGTGAAGAGCGGACCAACGGCGTCTACaacat                                                                                   <  1:219031/166‑4 (MQ=255)                                                                                                                                                                                                                                                tGGATCAGCTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCACGAAGGAACCTTTGGCCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACaacat                                                                                   >  2:219031/1‑163 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                GGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGTACGGTCATTCTGGATCAACTGGGCGAAGAGCACATGAAAACCGGCAAGCCGATTTTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGT  >  NC_000913/4619821‑4620306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑