Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,359	C→T	I377I (ATC→ATT)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,359	0	C	T	100.0%	81.6 / NA	27	I377I (ATC→ATT)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/17);  total (10/17)

GCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACAT  >  NC_000913/4619114‑4619602                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    gcgcgcgcGAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCGGATACCGAAGGTTTTGTCAGGGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTa                                                                                                                                                                                                                                                <  2:17319/251‑1 (MQ=255)      cgcgAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGAGGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCg                                                                                                                                                                                                                                           >  1:152487/1‑251 (MQ=255)         gAAATTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCg                                                                                                                                                                                                                                        <  2:92664/251‑1 (MQ=255)           aaTTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGc                                                                                                                                                                                                                                      <  1:93857/251‑1 (MQ=255)            aTTGCAAGCAGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGGCGTATGGTAGCGGCACAAAAAGGCCCAACCGACGTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTGTTGTCAGTGAAATGGATACCCGCGCGCTGGGGAGGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCt                                                                                                                                                                                                                                     <  2:13513/251‑1 (MQ=255)                                aCGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGtttg                                                                                                                                                                                                                 <  2:208980/251‑4 (MQ=255)                                aCGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGtttg                                                                                                                                                                                                                 <  1:230908/251‑4 (MQ=255)                                               aaGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCAACCGACTGCGGTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGAGACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGAc                                                                                                                                                                                                  <  2:237023/251‑1 (MQ=255)                                                     atGGTCGTATGGTAGCGGCACAAAAAGGCCCAGCCGTCGTCGTTGAGAACTACGCGAAGGATCGGCCGACAGCGATGCTGACTAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCAGCTGACACCAGTGATGACAGCGTCGGCTTGTCTGATAAGGCGCGTTTGGGCGACCAGGTAGACGGTc                                                                                                                                                                                              <  2:249418/248‑1 (MQ=255)                                                      tGGTCGTAAGGTAGCGGCACAAAAAGGCCCAACCGACTTCGTTGAGCACTACGCGAAGTATCTGCCGCCAGCGATGCTGACGAACGCAGTCTATGCTGCAACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGAAATAGGCGGCGGTCGTCGTCCGACATCTGACACCATTGATTACAGCGTCGGCTTTACTGCTATGGCCCGTTTGGGCGACCAGATAGACggac                                                                                                                                                                                              >  1:172634/1‑246 (MQ=255)                                                                               ggCCCAACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGaa                                                                                                                                                                  <  1:133100/251‑1 (MQ=255)                                                                                             cGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGGAaacagct                                                                                                                                                    >  1:69913/1‑248 (MQ=255)                                                                                                                              gATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCCTTGATTACAGCGTCGGCTTTACTGATATGGCGCGGTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAATGCGAAAACAGCCGGCAGGAAGGGGCGaaaaggggggaagcggggc                                                                                                                   >  2:135155/1‑235 (MQ=255)                                                                                                                                      cGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGGCGAAAACAGCTGGCAGGAAGCGGCGAAAACGGTGAAAGCGGGAATTaaaatt                                                                                                           >  2:70286/1‑248 (MQ=255)                                                                                                                                                      tGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGAAGAAAAAAGCTGGCAGGAAGCGGGGAAAGCGGTGGAAGCGGCAATTAAACTTGCCGATAAAGAACCgg                                                                                           >  1:177147/1‑251 (MQ=255)                                                                                                                                                                            gAAATGGATTCCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCg                                                                     <  2:83814/251‑1 (MQ=255)                                                                                                                                                                                     ccccGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAg                                                            <  1:1020/250‑1 (MQ=255)                                                                                                                                                                                          cgggcTGGGGATGGCAGTGGTTGCAATGGGCAGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGTCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGATGGCGGTTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCTGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGGCTAGCGCCGTATCAGCGAAt                                                       <  2:51015/248‑1 (MQ=255)                                                                                                                                                                                           gcgcTGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGGCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATa                                                      <  1:132595/251‑1 (MQ=255)                                                                                                                                                                                               tGGGGATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATCTGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGAGAAAGACGAAAACAGCTGGCAGGGAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACgg                                                  >  1:157100/1‑251 (MQ=255)                                                                                                                                                                                                ggggATGGCAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGGTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACgg                                                  <  1:182724/250‑1 (MQ=255)                                                                                                                                                                                                        cAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCCCGCGAAAGACg                                                                                                                                                             >  1:81677/1‑135 (MQ=255)                                                                                                                                                                                                        cAGTGGTTGCAATGGGCGGCGGTCGTCGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACg                                                                                                                                                             <  2:81677/135‑1 (MQ=255)                                                                                                                                                                                                                gCAATGGGCGGCTGTCGTCGTCAGGCATCTGACACCATTGAGTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGGCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCa                                  <  2:146923/250‑1 (MQ=255)                                                                                                                                                                                                                                  cGTCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGAAGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGGGAAAACGGCAATTAAAATTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCCTTTAAATTTGAAGCACTgg               >  1:143201/1‑250 (MQ=255)                                                                                                                                                                                                                                    tCAGGCATCTGACACCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGGAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAACACCCGAAAGCACACCAACTGTCTATCGCCGTATCAACCAATAAAGGTATACTGATCTGATCATTTAAATTTTAAGCACTGAgg             >  1:24114/1‑250 (MQ=255)                                                                                                                                                                                                                                               acacCATTGATTACAGCGTCGGCTTTACTGATATGGCGCGTTTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAGCTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGTTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACat  <  1:16774/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    GCGCGCGCGAAATTGCAGGCGGTGCTGGACAACGGTAAAGCGGCAGAAGTCTTTGGTCGTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAAGACGAAAACAACTGGCAGGAAGCGGCGAAAGCGGTGAAAGCGGCAATTAAACTTGCCGATAAAGCACCGGAAAGCACACCAACTGTCTATCGCCGTATCAGCGAATAACGGTATACTGATCTGATCATTTAAATTTGAAGCACTGAGTACGGAGAACAT  >  NC_000913/4619114‑4619602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑